Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates
Sea cucumber (Stichopus horrens) protein hydrolysates were known as a potential functional food source with angiotensinconverting enzyme (ACE) inhibitory. The genotoxicity properties of S. horrens protein hydrolysates have been investigated through two different in vitro tests: Ames test and in vi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2015
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| Online Access: | http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/1/44_1_01.pdf |
| _version_ | 1848812354734653440 |
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| author | Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah, |
| author_facet | Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah, |
| author_sort | Obren James Kawanding, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Sea cucumber (Stichopus horrens) protein hydrolysates were known as a potential functional food source with angiotensinconverting
enzyme (ACE) inhibitory. The genotoxicity properties of S. horrens protein hydrolysates have been investigated
through two different in vitro tests: Ames test and in vitro micronucleus test while the cytotoxicity properties of S. horrens
protein hydrolysate were assessed using neutral red test. The study was conducted at a concentration up to 8000 μg/ml, 80
μg/ml and 50 μg/ml for Ames test, in vitro micronucleus test and neutral red test respectively with and without metabolic
activation. There were no increments in the number of revertant colonies observed at any concentrations of S. horrens protein
hydrolysates with and without metabolic activation in all four strains of Salmonella typhimurium (TA98, TA100, TA1535
and TA1537) compared to the solvent control. In in vitro micronucleus test, S. horrens protein hydrolysate did not induce
clastogenicity in V79 cell while in neutral red test, S. horrens protein hydrolysate did not show any cytotoxic effects on
NIH/3T3 mouse fibroblast cell. In conclusion, S. horrens protein hydrolysates are safe in terms of genotoxic and cytotoxic
hence have the potential to be used in pharmaceutical and food industries as functional ingredient. |
| first_indexed | 2025-11-15T00:00:58Z |
| format | Article |
| id | oai:generic.eprints.org:10335 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:00:58Z |
| publishDate | 2015 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:103352017-04-21T09:19:10Z http://journalarticle.ukm.my/10335/ Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah, Sea cucumber (Stichopus horrens) protein hydrolysates were known as a potential functional food source with angiotensinconverting enzyme (ACE) inhibitory. The genotoxicity properties of S. horrens protein hydrolysates have been investigated through two different in vitro tests: Ames test and in vitro micronucleus test while the cytotoxicity properties of S. horrens protein hydrolysate were assessed using neutral red test. The study was conducted at a concentration up to 8000 μg/ml, 80 μg/ml and 50 μg/ml for Ames test, in vitro micronucleus test and neutral red test respectively with and without metabolic activation. There were no increments in the number of revertant colonies observed at any concentrations of S. horrens protein hydrolysates with and without metabolic activation in all four strains of Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) compared to the solvent control. In in vitro micronucleus test, S. horrens protein hydrolysate did not induce clastogenicity in V79 cell while in neutral red test, S. horrens protein hydrolysate did not show any cytotoxic effects on NIH/3T3 mouse fibroblast cell. In conclusion, S. horrens protein hydrolysates are safe in terms of genotoxic and cytotoxic hence have the potential to be used in pharmaceutical and food industries as functional ingredient. Penerbit Universiti Kebangsaan Malaysia 2015-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10335/1/44_1_01.pdf Obren James Kawanding, and Noriham Abdullah, and Zainon Mohd Noor, and Nooraain Hashim, and Nazamid Saari, and Mohd Fakharul Zaman Raja Yahya, and Mohd Faiz Foong Abdullah, (2015) Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates. Malaysian Applied Biology, 44 (3). pp. 1-4. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=520&catid=59:current-view&Itemid=56 |
| spellingShingle | Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah, Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates |
| title | Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates |
| title_full | Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates |
| title_fullStr | Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates |
| title_full_unstemmed | Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates |
| title_short | Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates |
| title_sort | genotoxicity and cytotoxicity evaluation of sea cucumber (stichopus horrens) protein hydrolysates |
| url | http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/1/44_1_01.pdf |