Understanding the ZEB1 interactome during lymphangiogenesis
Endothelial cells (ECs) constitute the crucial lining of blood and lymphatic vessels, controlling vascular integrity and function. During quiescence, ECs regulate junctional integrity and barrier stability. Recently, ZEB1 was hypothesised to contribute to endothelial quiescence. Integrating mass spe...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2025
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| Online Access: | https://eprints.nottingham.ac.uk/80747/ |
| _version_ | 1848801268983660544 |
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| author | Ahmed, Nada |
| author_facet | Ahmed, Nada |
| author_sort | Ahmed, Nada |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Endothelial cells (ECs) constitute the crucial lining of blood and lymphatic vessels, controlling vascular integrity and function. During quiescence, ECs regulate junctional integrity and barrier stability. Recently, ZEB1 was hypothesised to contribute to endothelial quiescence. Integrating mass spectrometric and transcriptomic analyses in lymphatic ECs uncovered that ZEB1 contributes to junctional reorganization. Silencing ZEB1 significantly reduced VE-cadherin phosphorylation at Y731 and Y685 (59%±5.39, 71%±15,p<0.05, respectively), consequently compromising barrier integrity. Silencing ZEB1 downregulated YES and YAP1 suggesting that altered VE-cadherin signalling was a consequence of ZEB1 mediated-YES/YAP1 signalling. In LECs, silencing YAP1 induced cytoskeletal remodelling, and functionally compromised EC barrier phenocopying ZEB1 loss. Consistently, YAP1 loss showed dephosphorylation of Y685 and Y731. |
| first_indexed | 2025-11-14T21:04:46Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-80747 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:04:46Z |
| publishDate | 2025 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-807472025-07-23T04:40:11Z https://eprints.nottingham.ac.uk/80747/ Understanding the ZEB1 interactome during lymphangiogenesis Ahmed, Nada Endothelial cells (ECs) constitute the crucial lining of blood and lymphatic vessels, controlling vascular integrity and function. During quiescence, ECs regulate junctional integrity and barrier stability. Recently, ZEB1 was hypothesised to contribute to endothelial quiescence. Integrating mass spectrometric and transcriptomic analyses in lymphatic ECs uncovered that ZEB1 contributes to junctional reorganization. Silencing ZEB1 significantly reduced VE-cadherin phosphorylation at Y731 and Y685 (59%±5.39, 71%±15,p<0.05, respectively), consequently compromising barrier integrity. Silencing ZEB1 downregulated YES and YAP1 suggesting that altered VE-cadherin signalling was a consequence of ZEB1 mediated-YES/YAP1 signalling. In LECs, silencing YAP1 induced cytoskeletal remodelling, and functionally compromised EC barrier phenocopying ZEB1 loss. Consistently, YAP1 loss showed dephosphorylation of Y685 and Y731. 2025-07-23 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/80747/1/AHMED%2CNADA%2C20217798%2CFinal%20Thesis.pdf Ahmed, Nada (2025) Understanding the ZEB1 interactome during lymphangiogenesis. PhD thesis, University of Nottingham. Lymphangiogenesis ZEB1 Vascular biology Interactome Junctional integrity |
| spellingShingle | Lymphangiogenesis ZEB1 Vascular biology Interactome Junctional integrity Ahmed, Nada Understanding the ZEB1 interactome during lymphangiogenesis |
| title | Understanding the ZEB1 interactome during lymphangiogenesis |
| title_full | Understanding the ZEB1 interactome during lymphangiogenesis |
| title_fullStr | Understanding the ZEB1 interactome during lymphangiogenesis |
| title_full_unstemmed | Understanding the ZEB1 interactome during lymphangiogenesis |
| title_short | Understanding the ZEB1 interactome during lymphangiogenesis |
| title_sort | understanding the zeb1 interactome during lymphangiogenesis |
| topic | Lymphangiogenesis ZEB1 Vascular biology Interactome Junctional integrity |
| url | https://eprints.nottingham.ac.uk/80747/ |