Recombinant expression of native Tritrypticin and optimisation of an In vivo biosynthesis platform for high-throughput peptide therapeutics discovery

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Recombinant expression of native Tritrypticin and optimisation of an In vivo biosynthesis platform for high-throughput peptide therapeutics discovery

The global increase in antibiotic-resistant bacteria poses a public health threat, heightened by overuse of antibiotics in livestock farming, the food industry, and healthcare. This demands for novel antimicrobial compounds to address the diminishing inefficacy of current antibiotics. Antimicrobial...

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Bibliographic Details
Main Author:	Mahendran, Arun Suria Karnan
Format:	Thesis (University of Nottingham only)
Language:	English
Published:	2025
Subjects:	antibiotic resistance; antimicrobial peptides; Tritrypticin; Synechocystis sp. DnaB intein; directed evolution; error-prone PCR; E. coli BL21 (DE3); pTWIN1 plasmid; outer membrane protein A (OmpA); novel signal peptide 4 (NSP4); recombinant antimicrobial peptide production; recombinant expression optimisation; high-throughput screening assays; bacterial agar screening; mutant peptide library screening; enhanced mutant AMP activity; inducible intein-mediated experssion system; antimictobial inhibition zones
Online Access:	https://eprints.nottingham.ac.uk/80269/

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