Identification of novel survivin interactors

Survivin, a multifaceted protein, plays crucial roles in various cellular processes, including apoptosis inhibition, mitosis, autophagy, and mitochondrial dynamics.Its overexpression has been linked to cancer cell proliferation, angiogenesis, chemotherapy resistance, and poor prognosis in multiple...

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Main Author: Alhawsawi, Naif
Format: Thesis (University of Nottingham only)
Language:English
Published: 2024
Subjects:
Online Access:https://eprints.nottingham.ac.uk/79899/
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author Alhawsawi, Naif
author_facet Alhawsawi, Naif
author_sort Alhawsawi, Naif
building Nottingham Research Data Repository
collection Online Access
description Survivin, a multifaceted protein, plays crucial roles in various cellular processes, including apoptosis inhibition, mitosis, autophagy, and mitochondrial dynamics.Its overexpression has been linked to cancer cell proliferation, angiogenesis, chemotherapy resistance, and poor prognosis in multiple sclerosis. While primarily known for its roles in programmed cell death and cell division, recent evidence suggests its involvement in clathrin-mediated endocytosis trafficking. To explore survivin's interactions and potential functions, we conducted protein-protein interaction studies. We identified two novel interactors, AP1g1 and Seh1, and confirmed a previously reported interaction with CHC. Our findings indicate that survivin directly interacts with AP1g1 and Seh1, while its interaction with CHC is indirect. Additionally, we observed that survivin overexpression downregulates the expression of CNP and PDGFR-a, two myelin-related genes regulated by Seh1 indicating a potential survivin role.These results collectively suggest that survivin may play a role in trafficking and in regulating the myelination of the central nervous system.
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institution University of Nottingham Malaysia Campus
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spelling nottingham-798992024-12-11T04:40:21Z https://eprints.nottingham.ac.uk/79899/ Identification of novel survivin interactors Alhawsawi, Naif Survivin, a multifaceted protein, plays crucial roles in various cellular processes, including apoptosis inhibition, mitosis, autophagy, and mitochondrial dynamics.Its overexpression has been linked to cancer cell proliferation, angiogenesis, chemotherapy resistance, and poor prognosis in multiple sclerosis. While primarily known for its roles in programmed cell death and cell division, recent evidence suggests its involvement in clathrin-mediated endocytosis trafficking. To explore survivin's interactions and potential functions, we conducted protein-protein interaction studies. We identified two novel interactors, AP1g1 and Seh1, and confirmed a previously reported interaction with CHC. Our findings indicate that survivin directly interacts with AP1g1 and Seh1, while its interaction with CHC is indirect. Additionally, we observed that survivin overexpression downregulates the expression of CNP and PDGFR-a, two myelin-related genes regulated by Seh1 indicating a potential survivin role.These results collectively suggest that survivin may play a role in trafficking and in regulating the myelination of the central nervous system. 2024-12-11 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/79899/1/Alhawsaw%2C%20Naif%2C%202013261%2C%20Correction%20Submession.pdf Alhawsawi, Naif (2024) Identification of novel survivin interactors. PhD thesis, University of Nottingham. Survivin; Cellular processes; Trafficking
spellingShingle Survivin; Cellular processes; Trafficking
Alhawsawi, Naif
Identification of novel survivin interactors
title Identification of novel survivin interactors
title_full Identification of novel survivin interactors
title_fullStr Identification of novel survivin interactors
title_full_unstemmed Identification of novel survivin interactors
title_short Identification of novel survivin interactors
title_sort identification of novel survivin interactors
topic Survivin; Cellular processes; Trafficking
url https://eprints.nottingham.ac.uk/79899/