Methylocystis parvus: genomics, engineering and sustainable PHB production
Methylocystis parvus OBBP is a gram-negative aerobic methanotroph of the phylum Alphaproteobacteria. The type species of its genus and type strain of its species. Methanotrophs are industrially relevant organisms defined by their ability to use methane as their sole carbon and energy source. Among m...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2024
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| Online Access: | https://eprints.nottingham.ac.uk/77436/ |
| _version_ | 1848800998943883264 |
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| author | Claxton Stevens, Benedict H. |
| author_facet | Claxton Stevens, Benedict H. |
| author_sort | Claxton Stevens, Benedict H. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Methylocystis parvus OBBP is a gram-negative aerobic methanotroph of the phylum Alphaproteobacteria. The type species of its genus and type strain of its species. Methanotrophs are industrially relevant organisms defined by their ability to use methane as their sole carbon and energy source. Among many qualities M. parvus is capable of the production of poly(3-hydroxybutyrate) (PHB) which is biodegradable and if produced from biogas, is a bioplastic meaning it is produced from renewable biomass. PHB produced by methanotrophs thus has the potential to aid in the current climate crises of plastic waste and greenhouse gas emission fitting into a circular carbon economy.
Across this thesis this goal is developed towards first through genetics assembling a full genome of M. parvus OBBP and demonstrating CRISPR genetic editing in it. This forms the first published genetic editing in M. parvus. This is followed by chapters investigating PHB fermentation in methanotrophs particularly using real-world biogas comparing multiple strains and scaling production up to 1L fermentation bioreactors. A nanopore based 16S sequencing method for investigation of mixed culture fermentation species abundance is also demonstrated evaluating two PHB producing mixed cultures. Work in this thesis has contributed to three published papers which are included at the end. |
| first_indexed | 2025-11-14T21:00:28Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-77436 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:00:28Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-774362024-08-23T15:06:49Z https://eprints.nottingham.ac.uk/77436/ Methylocystis parvus: genomics, engineering and sustainable PHB production Claxton Stevens, Benedict H. Methylocystis parvus OBBP is a gram-negative aerobic methanotroph of the phylum Alphaproteobacteria. The type species of its genus and type strain of its species. Methanotrophs are industrially relevant organisms defined by their ability to use methane as their sole carbon and energy source. Among many qualities M. parvus is capable of the production of poly(3-hydroxybutyrate) (PHB) which is biodegradable and if produced from biogas, is a bioplastic meaning it is produced from renewable biomass. PHB produced by methanotrophs thus has the potential to aid in the current climate crises of plastic waste and greenhouse gas emission fitting into a circular carbon economy. Across this thesis this goal is developed towards first through genetics assembling a full genome of M. parvus OBBP and demonstrating CRISPR genetic editing in it. This forms the first published genetic editing in M. parvus. This is followed by chapters investigating PHB fermentation in methanotrophs particularly using real-world biogas comparing multiple strains and scaling production up to 1L fermentation bioreactors. A nanopore based 16S sequencing method for investigation of mixed culture fermentation species abundance is also demonstrated evaluating two PHB producing mixed cultures. Work in this thesis has contributed to three published papers which are included at the end. 2024-07-16 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/77436/1/Final%20Thesis%20With%20Papers.pdf Claxton Stevens, Benedict H. (2024) Methylocystis parvus: genomics, engineering and sustainable PHB production. PhD thesis, University of Nottingham. methanotrophs CRISPR genome editing methane monooxygenase genome sequencing methanotrophy Methylocystis species Poly-3-hydroxybutyrate PHB Bioplastic Biogas gas fermentation |
| spellingShingle | methanotrophs CRISPR genome editing methane monooxygenase genome sequencing methanotrophy Methylocystis species Poly-3-hydroxybutyrate PHB Bioplastic Biogas gas fermentation Claxton Stevens, Benedict H. Methylocystis parvus: genomics, engineering and sustainable PHB production |
| title | Methylocystis parvus: genomics, engineering and sustainable PHB production |
| title_full | Methylocystis parvus: genomics, engineering and sustainable PHB production |
| title_fullStr | Methylocystis parvus: genomics, engineering and sustainable PHB production |
| title_full_unstemmed | Methylocystis parvus: genomics, engineering and sustainable PHB production |
| title_short | Methylocystis parvus: genomics, engineering and sustainable PHB production |
| title_sort | methylocystis parvus: genomics, engineering and sustainable phb production |
| topic | methanotrophs CRISPR genome editing methane monooxygenase genome sequencing methanotrophy Methylocystis species Poly-3-hydroxybutyrate PHB Bioplastic Biogas gas fermentation |
| url | https://eprints.nottingham.ac.uk/77436/ |