Molecular biology of human DDX49 - novel biochemical activities
DExD-box helicases belong to superfamily II of RNA helicases and are characterised by a conserved Asp-Glu-X-Asp motif in their primary protein sequence. These helicases are often involved in pre-mRNA splicing, ribosome biogenesis and also cell cycle mediation; as such, they are implicated in the onc...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2023
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| Online Access: | https://eprints.nottingham.ac.uk/72946/ |
| _version_ | 1848800754230362112 |
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| author | Anandavijayan, Sabesan |
| author_facet | Anandavijayan, Sabesan |
| author_sort | Anandavijayan, Sabesan |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | DExD-box helicases belong to superfamily II of RNA helicases and are characterised by a conserved Asp-Glu-X-Asp motif in their primary protein sequence. These helicases are often involved in pre-mRNA splicing, ribosome biogenesis and also cell cycle mediation; as such, they are implicated in the oncogenesis and proliferation of various cancer types. The overexpression of DEAD-box protein 49 (DDX49) has been associated with a favourable prognosis in cervical cancer patients, however, its structure and function has yet to be fully elucidated. We report that DDX49 is a dual function helicase and nuclease, and discuss how CRISPR-Cas9 mediated downregulation of DDX49 may affect the proliferation and viability of U2OS osteosarcoma cells. |
| first_indexed | 2025-11-14T20:56:35Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-72946 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:56:35Z |
| publishDate | 2023 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-729462023-07-31T04:40:41Z https://eprints.nottingham.ac.uk/72946/ Molecular biology of human DDX49 - novel biochemical activities Anandavijayan, Sabesan DExD-box helicases belong to superfamily II of RNA helicases and are characterised by a conserved Asp-Glu-X-Asp motif in their primary protein sequence. These helicases are often involved in pre-mRNA splicing, ribosome biogenesis and also cell cycle mediation; as such, they are implicated in the oncogenesis and proliferation of various cancer types. The overexpression of DEAD-box protein 49 (DDX49) has been associated with a favourable prognosis in cervical cancer patients, however, its structure and function has yet to be fully elucidated. We report that DDX49 is a dual function helicase and nuclease, and discuss how CRISPR-Cas9 mediated downregulation of DDX49 may affect the proliferation and viability of U2OS osteosarcoma cells. 2023-07-31 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/72946/1/Molecular%20Biology%20of%20DDX49%20-%20Novel%20Biochemical%20Activities%20%28Corrected%29.pdf Anandavijayan, Sabesan (2023) Molecular biology of human DDX49 - novel biochemical activities. MRes thesis, University of Nottingham. DEAD-box protein 49; Cell proliferation; Dual function helicase and nuclease |
| spellingShingle | DEAD-box protein 49; Cell proliferation; Dual function helicase and nuclease Anandavijayan, Sabesan Molecular biology of human DDX49 - novel biochemical activities |
| title | Molecular biology of human DDX49 - novel biochemical activities |
| title_full | Molecular biology of human DDX49 - novel biochemical activities |
| title_fullStr | Molecular biology of human DDX49 - novel biochemical activities |
| title_full_unstemmed | Molecular biology of human DDX49 - novel biochemical activities |
| title_short | Molecular biology of human DDX49 - novel biochemical activities |
| title_sort | molecular biology of human ddx49 - novel biochemical activities |
| topic | DEAD-box protein 49; Cell proliferation; Dual function helicase and nuclease |
| url | https://eprints.nottingham.ac.uk/72946/ |