Roles of post-transcriptional modifications in Solanum lycopersicum
Methylation at the N6 position of internal adenosines (m6A) is known as the most prevalent and widespread modification in mRNA. In plants, m6A has roles in post-transcriptional gene control, influencing growth, development and reproduction. The function of these modifications is often mediated by RN...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2022
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| Online Access: | https://eprints.nottingham.ac.uk/69238/ |
| _version_ | 1848800548219781120 |
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| author | Grundy, Cameron H S |
| author_facet | Grundy, Cameron H S |
| author_sort | Grundy, Cameron H S |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Methylation at the N6 position of internal adenosines (m6A) is known as the most prevalent and widespread modification in mRNA. In plants, m6A has roles in post-transcriptional gene control, influencing growth, development and reproduction. The function of these modifications is often mediated by RNA binding proteins containing a YT521-B homology (YTH) domain. These domains contain a hydrophobic pocket that can directly accommodate m6A residues. Despite studies demonstrating that m6A is crucial for plant development and multiple studies on mammalian YTH proteins, very little is known about YTH proteins in plants.
Here, we have identified that Solanum lycopersicum have some of the lowest number of YTH proteins in Plantae. We show that plants lacking YTHDF1, YTHDF2, YTHDF3 or YTHDF4 from Solanum lycopersicum, exhibit vegetative growth defects, including phenotypes which overlap with those identified in low m6A plant lines and respond differently to salt stress. In addition, we have produced several YTHDF double mutants, which demonstrate that each YTHDF protein have both redundant and specific functions in vivo. Finally, we demonstrate a novel in vitro randomised RNA-binding protein selection strategy, which has the potential to be used to identify key consensus sequences to further our understanding of complex processes. This strategy allowed us to identify that just like their phenotypes, each YTHDF protein in tomato show affinity for both unique and overlapping sequences in vitro. This work provides a platform for future advances in our understanding of these complex proteins. |
| first_indexed | 2025-11-14T20:53:18Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-69238 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:53:18Z |
| publishDate | 2022 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-692382025-02-28T15:15:29Z https://eprints.nottingham.ac.uk/69238/ Roles of post-transcriptional modifications in Solanum lycopersicum Grundy, Cameron H S Methylation at the N6 position of internal adenosines (m6A) is known as the most prevalent and widespread modification in mRNA. In plants, m6A has roles in post-transcriptional gene control, influencing growth, development and reproduction. The function of these modifications is often mediated by RNA binding proteins containing a YT521-B homology (YTH) domain. These domains contain a hydrophobic pocket that can directly accommodate m6A residues. Despite studies demonstrating that m6A is crucial for plant development and multiple studies on mammalian YTH proteins, very little is known about YTH proteins in plants. Here, we have identified that Solanum lycopersicum have some of the lowest number of YTH proteins in Plantae. We show that plants lacking YTHDF1, YTHDF2, YTHDF3 or YTHDF4 from Solanum lycopersicum, exhibit vegetative growth defects, including phenotypes which overlap with those identified in low m6A plant lines and respond differently to salt stress. In addition, we have produced several YTHDF double mutants, which demonstrate that each YTHDF protein have both redundant and specific functions in vivo. Finally, we demonstrate a novel in vitro randomised RNA-binding protein selection strategy, which has the potential to be used to identify key consensus sequences to further our understanding of complex processes. This strategy allowed us to identify that just like their phenotypes, each YTHDF protein in tomato show affinity for both unique and overlapping sequences in vitro. This work provides a platform for future advances in our understanding of these complex proteins. 2022-08-03 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/69238/1/Cameron%20Grundy%20-%20Doctoral%20Thesis%20-%20Corrected%20.pdf Grundy, Cameron H S (2022) Roles of post-transcriptional modifications in Solanum lycopersicum. PhD thesis, University of Nottingham. Solanum lycopersicum Post-transcriptional modifications |
| spellingShingle | Solanum lycopersicum Post-transcriptional modifications Grundy, Cameron H S Roles of post-transcriptional modifications in Solanum lycopersicum |
| title | Roles of post-transcriptional modifications in Solanum lycopersicum |
| title_full | Roles of post-transcriptional modifications in Solanum lycopersicum |
| title_fullStr | Roles of post-transcriptional modifications in Solanum lycopersicum |
| title_full_unstemmed | Roles of post-transcriptional modifications in Solanum lycopersicum |
| title_short | Roles of post-transcriptional modifications in Solanum lycopersicum |
| title_sort | roles of post-transcriptional modifications in solanum lycopersicum |
| topic | Solanum lycopersicum Post-transcriptional modifications |
| url | https://eprints.nottingham.ac.uk/69238/ |