Isolation and identification of plastic degrading microorganism
Increased usage of plastic is leading to plastic pollution in the environment. Whilst plastics are believed to be non-biodegradable, it is believed that with long exposure to the environment, the microbiome in the environment may evolve to metabolise plastic. Many genera of microbes have been isolat...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English English English |
| Published: |
2021
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| Online Access: | https://eprints.nottingham.ac.uk/66097/ |
| _version_ | 1848800297410887680 |
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| author | Lee, Kwon Jun |
| author_facet | Lee, Kwon Jun |
| author_sort | Lee, Kwon Jun |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Increased usage of plastic is leading to plastic pollution in the environment. Whilst plastics are believed to be non-biodegradable, it is believed that with long exposure to the environment, the microbiome in the environment may evolve to metabolise plastic. Many genera of microbes have been isolated and tested for degradation of polyethylene terephthalate and polystyrene however, deeper understanding of the mechanism and the demand for novel plastic degrading microorganisms is higher than ever. In this work, TPA degrading Streptomyces sps. was isolated from soil samples collected near human sewage treatment plant by utilizing selective enrichment on the isolates. Identification of the strain was done using molecular approaches and phenotypic assays showed the plastic degrading potential of the isolate. Metabolic profiles of the isolate were assessed by LCMS with the results indicating difference in the culture grown in protocatechuic acid and terephthalic acid. |
| first_indexed | 2025-11-14T20:49:19Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-66097 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T20:49:19Z |
| publishDate | 2021 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-660972021-12-08T04:40:08Z https://eprints.nottingham.ac.uk/66097/ Isolation and identification of plastic degrading microorganism Lee, Kwon Jun Increased usage of plastic is leading to plastic pollution in the environment. Whilst plastics are believed to be non-biodegradable, it is believed that with long exposure to the environment, the microbiome in the environment may evolve to metabolise plastic. Many genera of microbes have been isolated and tested for degradation of polyethylene terephthalate and polystyrene however, deeper understanding of the mechanism and the demand for novel plastic degrading microorganisms is higher than ever. In this work, TPA degrading Streptomyces sps. was isolated from soil samples collected near human sewage treatment plant by utilizing selective enrichment on the isolates. Identification of the strain was done using molecular approaches and phenotypic assays showed the plastic degrading potential of the isolate. Metabolic profiles of the isolate were assessed by LCMS with the results indicating difference in the culture grown in protocatechuic acid and terephthalic acid. 2021-12-08 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/66097/10/10279197_Kwon%20Jun%20Lee.pdf application/pdf en cc_by https://eprints.nottingham.ac.uk/66097/11/response%20to%20examinars_10279197_Kwon%20Jun%20Lee.pdf application/pdf en cc_by https://eprints.nottingham.ac.uk/66097/2/D23PRO%20Undergraduate%20Research%20Project%20-%20Kwon%20Jun%20Lee%5B4616%5D.pdf Lee, Kwon Jun (2021) Isolation and identification of plastic degrading microorganism. MSc(Res) thesis, University of Nottingham. plastics pollution microbes microbial degradation Terephthalic acid |
| spellingShingle | plastics pollution microbes microbial degradation Terephthalic acid Lee, Kwon Jun Isolation and identification of plastic degrading microorganism |
| title | Isolation and identification of plastic degrading microorganism |
| title_full | Isolation and identification of plastic degrading microorganism |
| title_fullStr | Isolation and identification of plastic degrading microorganism |
| title_full_unstemmed | Isolation and identification of plastic degrading microorganism |
| title_short | Isolation and identification of plastic degrading microorganism |
| title_sort | isolation and identification of plastic degrading microorganism |
| topic | plastics pollution microbes microbial degradation Terephthalic acid |
| url | https://eprints.nottingham.ac.uk/66097/ |