MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS
Nano/microparticle based gene therapy, the next generation of therapeutics could potentially cure inherited and acquired diseases through delivering nucleic acid intracellularly which otherwise are difficult to treat. Developing non-viral gene therapy vectors that both protect and deliver nucleic ac...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2020
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| Online Access: | https://eprints.nottingham.ac.uk/63163/ |
| _version_ | 1848799998426218496 |
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| author | Jalal, Aveen |
| author_facet | Jalal, Aveen |
| author_sort | Jalal, Aveen |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Nano/microparticle based gene therapy, the next generation of therapeutics could potentially cure inherited and acquired diseases through delivering nucleic acid intracellularly which otherwise are difficult to treat. Developing non-viral gene therapy vectors that both protect and deliver nucleic acid cargoes efficiently will be vital if gene augmentation and editing strategies are to be effectively combined with advanced regenerative medicine approaches. Currently, these methodologies utilise high concentrations of recombinant growth factors, which result in toxicity and off-target effects. Herein, the use of modified cell penetrating peptides (CPPs), termed Glycosaminoglycan (GAG) binding Enhanced Transduction (GET) with plasmid DNA (pDNA) encapsulated poly (lactic-co-glycolic acid) PLGA nano/microparticles (pDNA encapsulated PLGA NP/MPs) is demonstrated. In order to encapsulate the pDNA in PLGA NP/MPs, it was first condensed with several condensing agents such as high and low molecular weight cationic polypeptides and alcohol. Low molecular weight Poly L-Lysine (PLL) produced the smallest and most homogenous population of pDNA nanoparticles (pDNA NPs) in the range of 10-50 nm. These pDNA NPs were then encapsulated in PLGA MPs by double emulsion methods; yielding encapsulation efficiencies (EE) of ~ 30 %. pDNA encapsulated PLGA MPs were in the range of 0.35 µm in diameter with a negative surface charge. PLGA MPs complexed with GET peptides show enhanced intracellular delivery (up to seven folds) and transfection efficiencies (up to five orders of magnitude). Moreover, the pDNA cargo has enhanced protection from nucleases (i.e. DNase I) promoting their translatability.
The biomedical applications of these MPs were tested in bone regeneration. For that, pBMP2 encapsulated PLGA-GET MPs were shown to efficiently deliver pBMP2 which can promote bone regeneration in human mesenchymal stem cells (hMSCs) in vitro. The bone lineage differentiations were confirmed by Alizarin red calcium staining and detection of bone lineage specific genes by Quantitative Reverse Transcription-Polymerase Chain Reaction (QRT-PCR).
By combining FDA-approved PLGA polymer nano/microtechnology with the GET delivery system, therapeutic non-viral vectors could have a significant impact on cellular therapy and regenerative medicine applications. |
| first_indexed | 2025-11-14T20:44:34Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-63163 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:44:34Z |
| publishDate | 2020 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-631632025-06-16T13:52:55Z https://eprints.nottingham.ac.uk/63163/ MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS Jalal, Aveen Nano/microparticle based gene therapy, the next generation of therapeutics could potentially cure inherited and acquired diseases through delivering nucleic acid intracellularly which otherwise are difficult to treat. Developing non-viral gene therapy vectors that both protect and deliver nucleic acid cargoes efficiently will be vital if gene augmentation and editing strategies are to be effectively combined with advanced regenerative medicine approaches. Currently, these methodologies utilise high concentrations of recombinant growth factors, which result in toxicity and off-target effects. Herein, the use of modified cell penetrating peptides (CPPs), termed Glycosaminoglycan (GAG) binding Enhanced Transduction (GET) with plasmid DNA (pDNA) encapsulated poly (lactic-co-glycolic acid) PLGA nano/microparticles (pDNA encapsulated PLGA NP/MPs) is demonstrated. In order to encapsulate the pDNA in PLGA NP/MPs, it was first condensed with several condensing agents such as high and low molecular weight cationic polypeptides and alcohol. Low molecular weight Poly L-Lysine (PLL) produced the smallest and most homogenous population of pDNA nanoparticles (pDNA NPs) in the range of 10-50 nm. These pDNA NPs were then encapsulated in PLGA MPs by double emulsion methods; yielding encapsulation efficiencies (EE) of ~ 30 %. pDNA encapsulated PLGA MPs were in the range of 0.35 µm in diameter with a negative surface charge. PLGA MPs complexed with GET peptides show enhanced intracellular delivery (up to seven folds) and transfection efficiencies (up to five orders of magnitude). Moreover, the pDNA cargo has enhanced protection from nucleases (i.e. DNase I) promoting their translatability. The biomedical applications of these MPs were tested in bone regeneration. For that, pBMP2 encapsulated PLGA-GET MPs were shown to efficiently deliver pBMP2 which can promote bone regeneration in human mesenchymal stem cells (hMSCs) in vitro. The bone lineage differentiations were confirmed by Alizarin red calcium staining and detection of bone lineage specific genes by Quantitative Reverse Transcription-Polymerase Chain Reaction (QRT-PCR). By combining FDA-approved PLGA polymer nano/microtechnology with the GET delivery system, therapeutic non-viral vectors could have a significant impact on cellular therapy and regenerative medicine applications. 2020-12-11 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/63163/1/Aveen%20Jalal%20eThesis.pdf Jalal, Aveen (2020) MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS. PhD thesis, University of Nottingham. nanoparticles gene therapy bone regeneration |
| spellingShingle | nanoparticles gene therapy bone regeneration Jalal, Aveen MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS |
| title | MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS |
| title_full | MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS |
| title_fullStr | MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS |
| title_full_unstemmed | MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS |
| title_short | MODIFIED CELL PENETRATING PEPTIDES FOR EFFICIENT POLYMER NANO/MICROPARTICLE DELIVERY FOR BONE DIFFERENTIATION APPLICATIONS |
| title_sort | modified cell penetrating peptides for efficient polymer nano/microparticle delivery for bone differentiation applications |
| topic | nanoparticles gene therapy bone regeneration |
| url | https://eprints.nottingham.ac.uk/63163/ |