Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells
The characteristic profile of CS-MSCs (Corneal Stroma Derived Mesenchymal Stem Cells) is growing in accordance with their increasing therapeutic interest. In vitro expansion of these cells involves basal media DMEM/F-12 supplemented with 10% FBS (Foetal Bovine Serum) or 10% HPL (Human Platelet Lysat...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2020
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| Online Access: | https://eprints.nottingham.ac.uk/60158/ |
| _version_ | 1848799735775756288 |
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| author | Ekrem, Melis |
| author_facet | Ekrem, Melis |
| author_sort | Ekrem, Melis |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The characteristic profile of CS-MSCs (Corneal Stroma Derived Mesenchymal Stem Cells) is growing in accordance with their increasing therapeutic interest. In vitro expansion of these cells involves basal media DMEM/F-12 supplemented with 10% FBS (Foetal Bovine Serum) or 10% HPL (Human Platelet Lysate). As animal derived serum can confer a risk of disease transmission, and is subject to batch to batch variability, this does not comply with cGMP (current Good Manufacturing Practice) required animal component – free culture protocols for clinical translation. CS-MSCs were additionally cultured in xeno-free and chemically defined media: Stem X Vivo, StemPro MSC SFM XF, Mesenchymal XF and StemMACS XF to determine whether CS-MSCs were able to proliferate at higher levels than in HPL medium and to aid the development of an optimal xeno-free growth media. CS-MSCs cultured in HPL were further subject to comparison with BM-MSCs (Bone Marrow Derived Mesenchymal Stem Cells) cultured in HPL media to assess MSC characteristics and to highlight which media could be determined as most successful for cell proliferation and morphology. |
| first_indexed | 2025-11-14T20:40:24Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-60158 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:40:24Z |
| publishDate | 2020 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-601582025-02-28T14:51:02Z https://eprints.nottingham.ac.uk/60158/ Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells Ekrem, Melis The characteristic profile of CS-MSCs (Corneal Stroma Derived Mesenchymal Stem Cells) is growing in accordance with their increasing therapeutic interest. In vitro expansion of these cells involves basal media DMEM/F-12 supplemented with 10% FBS (Foetal Bovine Serum) or 10% HPL (Human Platelet Lysate). As animal derived serum can confer a risk of disease transmission, and is subject to batch to batch variability, this does not comply with cGMP (current Good Manufacturing Practice) required animal component – free culture protocols for clinical translation. CS-MSCs were additionally cultured in xeno-free and chemically defined media: Stem X Vivo, StemPro MSC SFM XF, Mesenchymal XF and StemMACS XF to determine whether CS-MSCs were able to proliferate at higher levels than in HPL medium and to aid the development of an optimal xeno-free growth media. CS-MSCs cultured in HPL were further subject to comparison with BM-MSCs (Bone Marrow Derived Mesenchymal Stem Cells) cultured in HPL media to assess MSC characteristics and to highlight which media could be determined as most successful for cell proliferation and morphology. 2020-07-24 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/60158/1/MelisEkremMRes.pdf Ekrem, Melis (2020) Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells. MRes thesis, University of Nottingham. Corneal stroma derived mesenchymal stem cells; Cell culture; Cell proliferation; Xeno-free media |
| spellingShingle | Corneal stroma derived mesenchymal stem cells; Cell culture; Cell proliferation; Xeno-free media Ekrem, Melis Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells |
| title | Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells |
| title_full | Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells |
| title_fullStr | Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells |
| title_full_unstemmed | Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells |
| title_short | Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells |
| title_sort | development of an optimal xeno-free cgmp growth medium for the culture of corneal stroma-derived mesenchymal stem cells |
| topic | Corneal stroma derived mesenchymal stem cells; Cell culture; Cell proliferation; Xeno-free media |
| url | https://eprints.nottingham.ac.uk/60158/ |