Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses

Present work explores the relationship between the composition, dissolution rate, ion release and cytocompatibility of a series of borophosphate glasses. While, the base glass was selected to be 40mol%P2O5-16mol%CaO-24mol%MgO-20mol%Na2O, three B2O3 modified glass compositions were formulated by repl...

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Main Authors: Sharmin, Nusrat, Hasan, Mohammad S., Islam, Md. Towhidul, Pang, Chengheng, Gu, Fu, Parsons, Andrew J., Ahmed, Ifty
Format: Article
Language:English
Published: Walter de Gruyter GmbH 2019
Subjects:
Online Access:https://eprints.nottingham.ac.uk/59722/
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author Sharmin, Nusrat
Hasan, Mohammad S.
Islam, Md. Towhidul
Pang, Chengheng
Gu, Fu
Parsons, Andrew J.
Ahmed, Ifty
author_facet Sharmin, Nusrat
Hasan, Mohammad S.
Islam, Md. Towhidul
Pang, Chengheng
Gu, Fu
Parsons, Andrew J.
Ahmed, Ifty
author_sort Sharmin, Nusrat
building Nottingham Research Data Repository
collection Online Access
description Present work explores the relationship between the composition, dissolution rate, ion release and cytocompatibility of a series of borophosphate glasses. While, the base glass was selected to be 40mol%P2O5-16mol%CaO-24mol%MgO-20mol%Na2O, three B2O3 modified glass compositions were formulated by replacing Na2O with 1, 5 and 10 mol% B2O3. Ion release study was conducted using inductively coupled plasma atomic emission spectroscopy (ICP-AES). The thermal scans of the glasses as determined by differential scanning calorimetry (DSC) revealed an increment in the thermal properties with increasing B2O3 content in the glasses. On the other hand, the dissolution rate of the glasses decreased with increasing B2O3 content. To identify the effect of boron ion release on the cytocompatibility properties of the glasses, MG63 cells were cultured on the surface of the glass discs. The in vitro cell culture study suggested that glasses with 5 mol% B2O3 (P40B5) showed better cell proliferation and metabolic activity as compares to the glasses with 10 mol% (P40B10) or with no B2O3 (P40B0). The confocal laser scanning microscopy (CLSM) images of live/dead stained MG63 cells attached to the surface of the glasses also revealed that the number of dead cells attached to P40B5 glasses were significantly lower than both P40B0 and P40B10 glasses.
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spelling nottingham-597222020-01-09T02:56:36Z https://eprints.nottingham.ac.uk/59722/ Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses Sharmin, Nusrat Hasan, Mohammad S. Islam, Md. Towhidul Pang, Chengheng Gu, Fu Parsons, Andrew J. Ahmed, Ifty Present work explores the relationship between the composition, dissolution rate, ion release and cytocompatibility of a series of borophosphate glasses. While, the base glass was selected to be 40mol%P2O5-16mol%CaO-24mol%MgO-20mol%Na2O, three B2O3 modified glass compositions were formulated by replacing Na2O with 1, 5 and 10 mol% B2O3. Ion release study was conducted using inductively coupled plasma atomic emission spectroscopy (ICP-AES). The thermal scans of the glasses as determined by differential scanning calorimetry (DSC) revealed an increment in the thermal properties with increasing B2O3 content in the glasses. On the other hand, the dissolution rate of the glasses decreased with increasing B2O3 content. To identify the effect of boron ion release on the cytocompatibility properties of the glasses, MG63 cells were cultured on the surface of the glass discs. The in vitro cell culture study suggested that glasses with 5 mol% B2O3 (P40B5) showed better cell proliferation and metabolic activity as compares to the glasses with 10 mol% (P40B10) or with no B2O3 (P40B0). The confocal laser scanning microscopy (CLSM) images of live/dead stained MG63 cells attached to the surface of the glasses also revealed that the number of dead cells attached to P40B5 glasses were significantly lower than both P40B0 and P40B10 glasses. Walter de Gruyter GmbH 2019-12-02 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/59722/1/bglass-2019-0008.pdf Sharmin, Nusrat, Hasan, Mohammad S., Islam, Md. Towhidul, Pang, Chengheng, Gu, Fu, Parsons, Andrew J. and Ahmed, Ifty (2019) Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses. Biomedical Glasses, 5 (1). pp. 85-97. ISSN 2299-3932 Phosphate based glasses; ion release; glass dissolution; cytocompatibility; live/dead cells staining http://dx.doi.org/10.1515/bglass-2019-0008 doi:10.1515/bglass-2019-0008 doi:10.1515/bglass-2019-0008
spellingShingle Phosphate based glasses; ion release; glass dissolution; cytocompatibility; live/dead cells staining
Sharmin, Nusrat
Hasan, Mohammad S.
Islam, Md. Towhidul
Pang, Chengheng
Gu, Fu
Parsons, Andrew J.
Ahmed, Ifty
Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses
title Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses
title_full Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses
title_fullStr Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses
title_full_unstemmed Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses
title_short Effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses
title_sort effect of dissolution rate and subsequent ion release on cytocompatibility properties of borophosphate glasses
topic Phosphate based glasses; ion release; glass dissolution; cytocompatibility; live/dead cells staining
url https://eprints.nottingham.ac.uk/59722/
https://eprints.nottingham.ac.uk/59722/
https://eprints.nottingham.ac.uk/59722/