Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga
Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase...
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| Format: | Article |
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Elsevier
2019
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| Online Access: | https://eprints.nottingham.ac.uk/59649/ |
| _version_ | 1848799658897309696 |
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| author | Chew, Kit Wayne Chia, Shir Reen Krishnamoorthy, Rambabu Tao, Yang Chu, Dinh-Toi Show, Pau-Loke |
| author_facet | Chew, Kit Wayne Chia, Shir Reen Krishnamoorthy, Rambabu Tao, Yang Chu, Dinh-Toi Show, Pau-Loke |
| author_sort | Chew, Kit Wayne |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase forming components, system pH, volume ratio, air flotation time and crude extract concentration were evaluated to maximise the C-phycocyanin recovery yield and purity. The optimal conditions for the LBF system achieving C-phycocyanin purification fold of 3.49 compared to 2.43 from the initial LBF conditions was in polyethylene glycol (PEG) 4000 and potassium phosphate combination, with 250 g/L of polymer and salt concentration each, volume ratio of 1:0.85, system pH of 7.0, air flotation duration of 7 min and phycocyanin crude extract concentration of 0.625 %w/w. The LBF has effectively enhanced the purification of C-phycocyanin in a cost effective and simple processing. |
| first_indexed | 2025-11-14T20:39:10Z |
| format | Article |
| id | nottingham-59649 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:39:10Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
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| spelling | nottingham-596492021-05-19T04:30:13Z https://eprints.nottingham.ac.uk/59649/ Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga Chew, Kit Wayne Chia, Shir Reen Krishnamoorthy, Rambabu Tao, Yang Chu, Dinh-Toi Show, Pau-Loke Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase forming components, system pH, volume ratio, air flotation time and crude extract concentration were evaluated to maximise the C-phycocyanin recovery yield and purity. The optimal conditions for the LBF system achieving C-phycocyanin purification fold of 3.49 compared to 2.43 from the initial LBF conditions was in polyethylene glycol (PEG) 4000 and potassium phosphate combination, with 250 g/L of polymer and salt concentration each, volume ratio of 1:0.85, system pH of 7.0, air flotation duration of 7 min and phycocyanin crude extract concentration of 0.625 %w/w. The LBF has effectively enhanced the purification of C-phycocyanin in a cost effective and simple processing. Elsevier 2019-05-20 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/59649/1/Manucsript%20-%20PLShow_cleaned%20%5Baccepted%5D.pdf Chew, Kit Wayne, Chia, Shir Reen, Krishnamoorthy, Rambabu, Tao, Yang, Chu, Dinh-Toi and Show, Pau-Loke (2019) Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga. Bioresource Technology, 288 . ISSN 0960-8524 C-phycocyanin liquid biphasic flotation pigment purification Spirulina platensis http://www.sciencedirect.com/science/article/pii/S0960852419307497 121519 121519 |
| spellingShingle | C-phycocyanin liquid biphasic flotation pigment purification Spirulina platensis Chew, Kit Wayne Chia, Shir Reen Krishnamoorthy, Rambabu Tao, Yang Chu, Dinh-Toi Show, Pau-Loke Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga |
| title | Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga |
| title_full | Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga |
| title_fullStr | Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga |
| title_full_unstemmed | Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga |
| title_short | Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga |
| title_sort | liquid biphasic flotation for the purification of c-phycocyanin from spirulina platensis microalga |
| topic | C-phycocyanin liquid biphasic flotation pigment purification Spirulina platensis |
| url | https://eprints.nottingham.ac.uk/59649/ https://eprints.nottingham.ac.uk/59649/ https://eprints.nottingham.ac.uk/59649/ |