IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise
Exercise mimetics are pharmacological compounds that trigger pathways similar to those activated as a consequence of exercise training. They are therefore useful in pathological conditions where physical activity is beneficial but unfeasible. Models of exercise mimetics can also be used to study the...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2019
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| Online Access: | https://eprints.nottingham.ac.uk/59138/ |
| _version_ | 1848799591002013696 |
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| author | Forte, Marina |
| author_facet | Forte, Marina |
| author_sort | Forte, Marina |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Exercise mimetics are pharmacological compounds that trigger pathways similar to those activated as a consequence of exercise training. They are therefore useful in pathological conditions where physical activity is beneficial but unfeasible. Models of exercise mimetics can also be used to study the targets implicated in training or conversely, inactivity. In the study presented insulin-like growth factor-1 (IGF-1) is investigated as an in vitro model of exercise mimetics, plus or minus the addition of Exo1 drug, that should impair the crosstalk between endoplasmic reticulum and the Golgi apparatus to inquire in its role in exercise metabolism
In this study, cultures from the murine musculoskeletal cell line C2C12 are divided into three experimental groups for each exercise mimetics: a control group, a treated group and a treated group plus Exo1. Cells are treated for 24h and 48h.
IGF-1 increased myotube width at 24h by eliciting mTORC2, followed by mTORC1 at 48h. Hypertrophy was achieved independently of pMAPK.
IGF-1 overcompensated for Exo1-caused ER stress by inducing hypertrophy at 24h, but the effect was impaired after 48h. Hypertrophy at 24h was achieved through phosphorylation of ERK1/2 and pAkt. |
| first_indexed | 2025-11-14T20:38:06Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-59138 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:38:06Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-591382025-02-28T14:39:45Z https://eprints.nottingham.ac.uk/59138/ IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise Forte, Marina Exercise mimetics are pharmacological compounds that trigger pathways similar to those activated as a consequence of exercise training. They are therefore useful in pathological conditions where physical activity is beneficial but unfeasible. Models of exercise mimetics can also be used to study the targets implicated in training or conversely, inactivity. In the study presented insulin-like growth factor-1 (IGF-1) is investigated as an in vitro model of exercise mimetics, plus or minus the addition of Exo1 drug, that should impair the crosstalk between endoplasmic reticulum and the Golgi apparatus to inquire in its role in exercise metabolism In this study, cultures from the murine musculoskeletal cell line C2C12 are divided into three experimental groups for each exercise mimetics: a control group, a treated group and a treated group plus Exo1. Cells are treated for 24h and 48h. IGF-1 increased myotube width at 24h by eliciting mTORC2, followed by mTORC1 at 48h. Hypertrophy was achieved independently of pMAPK. IGF-1 overcompensated for Exo1-caused ER stress by inducing hypertrophy at 24h, but the effect was impaired after 48h. Hypertrophy at 24h was achieved through phosphorylation of ERK1/2 and pAkt. 2019-12-11 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/59138/1/IGF%201%2C%20AN%20IN%20VITRO%20SKELETAL%20MUSCLE%20CELL%20MODE%20OF%20EXERCISE%20MIMETICS%20TO%20INVESTIGATE%20THE%20ROLE%20OF%20EXOSOMAL%20TRAFFICKING%20IN%20REGULATING%20METABOLIC%20RESPONSES%20TO%20EXERCISE.pdf Forte, Marina (2019) IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise. MSc(Res) thesis, University of Nottingham. Exercise mimetics IGF-1 muscle molecular biology western blot EPS cell culture C2C12 |
| spellingShingle | Exercise mimetics IGF-1 muscle molecular biology western blot EPS cell culture C2C12 Forte, Marina IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise |
| title | IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise |
| title_full | IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise |
| title_fullStr | IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise |
| title_full_unstemmed | IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise |
| title_short | IGF1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise |
| title_sort | igf1: an in vitro skeletal muscle cell model of exercise mimetics to investigate the role of exosomal trafficking in regulating metabolic responses to exercise |
| topic | Exercise mimetics IGF-1 muscle molecular biology western blot EPS cell culture C2C12 |
| url | https://eprints.nottingham.ac.uk/59138/ |