NanoBRET to probe ligand-receptor and receptor-receptor interactions in living cells

QR Code

NanoBRET to probe ligand-receptor and receptor-receptor interactions in living cells

Increasing evidence has revealed the role of G protein-coupled receptors (GPCRs) in the regulation of signalling responses via complex cross-talk mechanisms with the receptor tyrosine kinase (RTK) family of transmembrane receptors. Vascular endothelial growth factor-a (VEGFa) mediates cancer angioge...

Full description

Bibliographic Details
Main Author:	Carvalheira Alcobia, Diana
Format:	Thesis (University of Nottingham only)
Language:	English
Published:	2019
Subjects:	NanoBRET Pharmacology Breast cancer
Online Access:	https://eprints.nottingham.ac.uk/56087/

Similar Items

Real-time ligand binding of fluorescent VEGF-A isoforms that discriminate between VEGFR2 and NRP1 in living cells
by: Peach, Chloe J., et al.
Published: (2018)

Real-time analysis of the binding of fluorescent VEGF₁₆₅a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes
by: Kilpatrick, Laura E., et al.
Published: (2017)

Investigating ligand-directed signalling at the cannabinoid receptor 2
by: Hourani, Wafa
Published: (2019)

The biological heterogeneity of oestrogen receptor positive breast cancer and its phenotypic characterisation
by: Habashy, Hany Onsy Fouad Ibrahim
Published: (2011)

Targeting the hypoxic expression of epidermal growth factor receptor
by: Alamoudi, Abdulmohsin
Published: (2021)

Use of a new proximity assay (NanoBRET) to investigate the ligand binding characteristics of three fluorescent ligands to the human β1-adrenoceptor expressed in HEK-293 cells
by: Soave, Mark, et al.
Published: (2016)

The role of polymeric immunoglobulin receptor in breast cancer
by: Asanprakit, Wichitra
Published: (2021)

Effects of disrupting insulin-like growth factor-1 receptor (IGF-1R) signal transduction pathways with inhibitors of IGF-1R, EGFR, PI3K, MEK, and MTOR in breast cancer cell lines
by: Ayub, Ayunadirah
Published: (2014)

Dynamics of ligand and nanobody binding at CXCR4/EGFR complexes
by: Comez, Dehan
Published: (2025)

Chemokine (C-C motif) receptor 7 (CCR7) associates with the tumour immune microenvironment but not progression in invasive breast carcinoma
by: Sonbul, Sultan N., et al.
Published: (2017)

Synthesis and evaluation of the first fluorescent antagonists of the human P2Y2 receptor based on AR-C118925
by: Conroy, Sean, et al.
Published: (2018)

Investigating cooperativity across adenosine A3 receptor homo- and hetero-oligomers
by: Groenewoud, N.J.A.
Published: (2019)

Molecular modeling of muscarinic acetylcholine receptors: Structural basis of ligand-receptor interactions / Chin Sek Peng
by: Chin, Sek Peng
Published: (2015)

Design, synthesis and pharmacological evaluation of novel fluorescent ligands for the mu-opioid receptor
by: Marshall, William
Published: (2021)

Polymorphisms in Estrogen Receptor-Α and -Β Genes and Their Correlations with Risk Factors in Iranian Breast Cancer Patients
by: Abbasi, Sakineh
Published: (2009)

Oestrogen receptors in oesophageal cancer
by: De Rosa, Antonella
Published: (2018)

Translational control of Human Epidermal Growth Factor Receptors, HER2 and HER4 in response to cellular stresses in breast cancer cells
by: Edwards, Arnelle
Published: (2023)

Imaging Cannabinoid Receptors: A Brief Collection of Covalent and Fluorescent Probes for CB1 and CB2 Receptors
by: Hamilton, A.J., et al.
Published: (2021)

Small molecule-based fluorescent probes as tool compounds for chemokine receptors CXCR4 and ACKR3
by: Dekkers, Sebastian
Published: (2019)

Multimodal assessment of estrogen receptor mRNA profiles to quantify estrogen pathway activity in breast tumors
by: Muthukaruppan, Anita, et al.
Published: (2017)

Novel fenofibrate derivatives as cannabinoid receptor ligands
by: Spencer, Sarah Jane
Published: (2011)

Angiotensin II receptor blocker valsartan enhances glucose-induced insulin secretion
by: Ayob, Azizi, et al.
Published: (2006)

Application of BRET to monitor ligand binding to GPCRs
by: Stoddart, Leigh A., et al.
Published: (2015)

Probing the interaction of Hepatitis C virus glycoproteins with putative receptors and neutralising antibodies
by: Mirza, Deeman
Published: (2012)

Extrapyramidal side effects of antipsychotics are linked to their association kinetics at dopamine D2 receptors
by: Sykes, David A., et al.
Published: (2017)

Effects of silencing dishevelled 1, 2 and 3 mRNA expression on MDA-MB-231 cell migration and invasion using siRNAs
by: Khoo, Samuel Leon Juan
Published: (2015)

Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats
by: Mizher, Hussam A., et al.
Published: (2014)

Effect of cannabinoid receptor ligands on microglial cell functions
by: Hassan, Samia
Published: (2013)

Potency and species specificity of aryl hydrocarbon receptor ligands
by: Wall, Richard John
Published: (2012)

Fibroblast growth factor receptor 4 (FGF4) and fibroblast growth factor 19 (FGFR19) autocrine enhance brest cancer cells survival
by: Tiong, Kai Hung, et al.
Published: (2016)

Molecular Probes for P2X7 Receptor Studies
by: Gunosewoyo, Hendra, et al.
Published: (2007)

Illuminating the life of the adenosine receptors: development and application of subtype-selective fluorescent probes for the study of the human A1 and A2A adenosine receptors in living cells
by: Comeo, Eleonora
Published: (2022)

Characterisation of Recombinant Aryl Hydrocarbon Receptor Ligand Binding Domain
by: Jiang, Tao
Published: (2004)

Cell adhesion: stochastic receptor-ligand binding under force
by: Bagdasar, Ovidiu
Published: (2011)

Anti-breast cancer effect of eupatorin in vitro and in vivo
by: Abd Razak, Nursyamirah
Published: (2018)

The outcome of breast cancer based on the expression of c-erbb2 , estrogen receptor and progesterone receptor: kelantan experience 2002-2004
by: Mohamed, Moharzudi
Published: (2010)

Health-related quality of life from the FALCON phase III randomised trial of fulvestrant 500 mg versus anastrozole for hormone receptor-positive advanced breast cancer
by: Robertson, John F.R., et al.
Published: (2018)

Development of novel fluorescent histamine H₁-receptor antagonists to study ligand-binding kinetics in living cells
by: Stoddart, Leigh A., et al.
Published: (2018)

PIK3C Agene mutations in breast carcinoma in Malaysian patients
by: Leong, Vincent Ching-Shian, et al.
Published: (2008)

Overexpression of phospho-Akt correlates with phosphorylation of EGF receptor, FKHR and BAD in nasopharyngeal carcinoma
by: Yip , WK
Published: (2008)