Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection
We present a time-gated Raman micro-spectroscopy technique suitable for fast Raman mapping of samples eliciting large laser-induced fluorescence backgrounds. To achieve the required time resolution for effective fluorescence rejection, a picosecond pulsed laser and a single-photon avalanche diode we...
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| Format: | Article |
| Language: | English |
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Christopher Corden
2018
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| Online Access: | https://eprints.nottingham.ac.uk/55646/ |
| _version_ | 1848799196767846400 |
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| author | Corden, Christopher Shipp, Dustin Matousek, Pavel Notingher, Ioan |
| author_facet | Corden, Christopher Shipp, Dustin Matousek, Pavel Notingher, Ioan |
| author_sort | Corden, Christopher |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We present a time-gated Raman micro-spectroscopy technique suitable for fast Raman mapping of samples eliciting large laser-induced fluorescence backgrounds. To achieve the required time resolution for effective fluorescence rejection, a picosecond pulsed laser and a single-photon avalanche diode were used. A module consisting of a spectrometer, digital micromirror device, and two prisms was used for high-resolution spectral filtering and multiplexing, which is required for a high chemical specificity and short integration times. With this instrument, we demonstrated time-gated Raman imaging of highly fluorescent samples, achieving acquisition times as short as 3 min for 40×40 pixel resolution images. |
| first_indexed | 2025-11-14T20:31:50Z |
| format | Article |
| id | nottingham-55646 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:31:50Z |
| publishDate | 2018 |
| publisher | Christopher Corden |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-556462018-12-04T15:33:30Z https://eprints.nottingham.ac.uk/55646/ Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection Corden, Christopher Shipp, Dustin Matousek, Pavel Notingher, Ioan We present a time-gated Raman micro-spectroscopy technique suitable for fast Raman mapping of samples eliciting large laser-induced fluorescence backgrounds. To achieve the required time resolution for effective fluorescence rejection, a picosecond pulsed laser and a single-photon avalanche diode were used. A module consisting of a spectrometer, digital micromirror device, and two prisms was used for high-resolution spectral filtering and multiplexing, which is required for a high chemical specificity and short integration times. With this instrument, we demonstrated time-gated Raman imaging of highly fluorescent samples, achieving acquisition times as short as 3 min for 40×40 pixel resolution images. Christopher Corden 2018-10-07 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/55646/1/Fast%20Raman%20spectral%20mapping%20of%20highly.pdf Corden, Christopher, Shipp, Dustin, Matousek, Pavel and Notingher, Ioan (2018) Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection. Optics Letters, 43 (23). pp. 5733-5736. ISSN 1539-4794 https://www.osapublishing.org/ol/fulltext.cfm?uri=ol-43-23-5733&id=401402 doi:10.1364/OL.43.005733 doi:10.1364/OL.43.005733 |
| spellingShingle | Corden, Christopher Shipp, Dustin Matousek, Pavel Notingher, Ioan Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection |
| title | Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection |
| title_full | Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection |
| title_fullStr | Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection |
| title_full_unstemmed | Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection |
| title_short | Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection |
| title_sort | fast raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection |
| url | https://eprints.nottingham.ac.uk/55646/ https://eprints.nottingham.ac.uk/55646/ https://eprints.nottingham.ac.uk/55646/ |