Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease
Despite major advances in the diagnosis of infections for many pathogenic organisms, there is still a problem obtaining an accurate immunodiagnosis of Echinococcus infection due to its serological cross-reactivity with other species of taeniid cestodes or at higher taxonomic levels. For this reason,...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2018
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| Online Access: | https://eprints.nottingham.ac.uk/55533/ |
| _version_ | 1848799178625384448 |
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| author | Barwary, Nafal |
| author_facet | Barwary, Nafal |
| author_sort | Barwary, Nafal |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Despite major advances in the diagnosis of infections for many pathogenic organisms, there is still a problem obtaining an accurate immunodiagnosis of Echinococcus infection due to its serological cross-reactivity with other species of taeniid cestodes or at higher taxonomic levels. For this reason, there are ongoing efforts to develop a better method for diagnosis of echinococcosis, especially when the parasite has a crucial role in hypersensitivity reactions. Like other helminthic infections, one of the immunological hallmarks is an elevated serum concentration of parasite-specific IgE.
Our aim was to assess the use of IgE reporter system as a possible new method for diagnosis Echinococcus spp infection, using RS-ATL8 NFAT Reporter System, which is a humanised rat basophilic leukaemia (RBL) cell line that can be used to detect the presence of specific human IgE directed against Echinococcus allergens and cross-link their receptors, depending on luciferase generation as an indication of presence of parasite-specific IgE, pointing to infection.
Towards this goal, we first optimised the use of the humanised RS-ATL8 Reporter System. This was achieved by optimisation of experimental conditions, such as cell density, stimulation time, optimum conditions for sensitising factors and stimulant optimum concentration. Once a robust standard operating procedure had been elaborated, the second goal was to choose a few Echinococcus antigens for the investigation into their immunogenic properties and potential diagnostic value and to express them recombinantly for testing through the RS-ATL8 NFAT Reporter System. The chosen antigens were EF1-alpha, EgAg5, AgB2, Cyclophilin, Eg19, EgTeg, and EgTPx. |
| first_indexed | 2025-11-14T20:31:32Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-55533 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:31:32Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-555332025-02-28T14:18:05Z https://eprints.nottingham.ac.uk/55533/ Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease Barwary, Nafal Despite major advances in the diagnosis of infections for many pathogenic organisms, there is still a problem obtaining an accurate immunodiagnosis of Echinococcus infection due to its serological cross-reactivity with other species of taeniid cestodes or at higher taxonomic levels. For this reason, there are ongoing efforts to develop a better method for diagnosis of echinococcosis, especially when the parasite has a crucial role in hypersensitivity reactions. Like other helminthic infections, one of the immunological hallmarks is an elevated serum concentration of parasite-specific IgE. Our aim was to assess the use of IgE reporter system as a possible new method for diagnosis Echinococcus spp infection, using RS-ATL8 NFAT Reporter System, which is a humanised rat basophilic leukaemia (RBL) cell line that can be used to detect the presence of specific human IgE directed against Echinococcus allergens and cross-link their receptors, depending on luciferase generation as an indication of presence of parasite-specific IgE, pointing to infection. Towards this goal, we first optimised the use of the humanised RS-ATL8 Reporter System. This was achieved by optimisation of experimental conditions, such as cell density, stimulation time, optimum conditions for sensitising factors and stimulant optimum concentration. Once a robust standard operating procedure had been elaborated, the second goal was to choose a few Echinococcus antigens for the investigation into their immunogenic properties and potential diagnostic value and to express them recombinantly for testing through the RS-ATL8 NFAT Reporter System. The chosen antigens were EF1-alpha, EgAg5, AgB2, Cyclophilin, Eg19, EgTeg, and EgTPx. 2018-12-11 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/55533/1/Thesis%20Final%20Version%2011-11-2018.pdf Barwary, Nafal (2018) Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease. PhD thesis, University of Nottingham. Parasitology Immunology Allergy Medicine Diagnosis Molecular Biology |
| spellingShingle | Parasitology Immunology Allergy Medicine Diagnosis Molecular Biology Barwary, Nafal Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease |
| title | Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease |
| title_full | Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease |
| title_fullStr | Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease |
| title_full_unstemmed | Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease |
| title_short | Use of the RS-ATL8 NFAT reporter system for diagnosis of hydatid disease |
| title_sort | use of the rs-atl8 nfat reporter system for diagnosis of hydatid disease |
| topic | Parasitology Immunology Allergy Medicine Diagnosis Molecular Biology |
| url | https://eprints.nottingham.ac.uk/55533/ |