A statistical view on calcium oscillations

Transient rises and falls of the intracellular calcium concentration have been observed in numerous cell types and under a plethora of conditions. There is now a growing body of evidence that these whole-cell calcium oscillations are stochastic, which poses a significant challenge for modelling. In...

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Main Authors: Powell, Jake, Falcke, Martin, Skupin, Alexander, Bellamy, Tomas C., Kypraios, Theodore, Thul, Ruediger
Format: Article
Language:English
Published: Springer 2018
Online Access:https://eprints.nottingham.ac.uk/55055/
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author Powell, Jake
Falcke, Martin
Skupin, Alexander
Bellamy, Tomas C.
Kypraios, Theodore
Thul, Ruediger
author_facet Powell, Jake
Falcke, Martin
Skupin, Alexander
Bellamy, Tomas C.
Kypraios, Theodore
Thul, Ruediger
author_sort Powell, Jake
building Nottingham Research Data Repository
collection Online Access
description Transient rises and falls of the intracellular calcium concentration have been observed in numerous cell types and under a plethora of conditions. There is now a growing body of evidence that these whole-cell calcium oscillations are stochastic, which poses a significant challenge for modelling. In this review, we take a closer look at recently developed statistical approaches to calcium oscillations. These models describe the timing of whole-cell calcium spikes, yet their parametrisations reflect subcellular processes. We show how non-stationary calcium spike sequences, which e.g. occur during slow depletion of intracellular calcium stores or in the presence of time-dependent stimulation, can be analysed with the help of so-called intensity functions. By utilising Bayesian concepts, we demonstrate how values of key parameters of the statistical model can be inferred from single cell calcium spike sequences and illustrate what information whole-cell statistical models can provide about the subcellular mechanistic processes that drive calcium oscillations. In particular, we find that the interspike interval distribution of HEK293 cells under constant stimulation is captured by a Gamma distribution.
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spelling nottingham-550552019-08-24T04:30:22Z https://eprints.nottingham.ac.uk/55055/ A statistical view on calcium oscillations Powell, Jake Falcke, Martin Skupin, Alexander Bellamy, Tomas C. Kypraios, Theodore Thul, Ruediger Transient rises and falls of the intracellular calcium concentration have been observed in numerous cell types and under a plethora of conditions. There is now a growing body of evidence that these whole-cell calcium oscillations are stochastic, which poses a significant challenge for modelling. In this review, we take a closer look at recently developed statistical approaches to calcium oscillations. These models describe the timing of whole-cell calcium spikes, yet their parametrisations reflect subcellular processes. We show how non-stationary calcium spike sequences, which e.g. occur during slow depletion of intracellular calcium stores or in the presence of time-dependent stimulation, can be analysed with the help of so-called intensity functions. By utilising Bayesian concepts, we demonstrate how values of key parameters of the statistical model can be inferred from single cell calcium spike sequences and illustrate what information whole-cell statistical models can provide about the subcellular mechanistic processes that drive calcium oscillations. In particular, we find that the interspike interval distribution of HEK293 cells under constant stimulation is captured by a Gamma distribution. Springer 2018-08-06 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/55055/1/AdvExpMedBiol.pdf Powell, Jake, Falcke, Martin, Skupin, Alexander, Bellamy, Tomas C., Kypraios, Theodore and Thul, Ruediger (2018) A statistical view on calcium oscillations. Advances in Experimental Medicine and Biology . ISSN 0065-2598 (In Press)
spellingShingle Powell, Jake
Falcke, Martin
Skupin, Alexander
Bellamy, Tomas C.
Kypraios, Theodore
Thul, Ruediger
A statistical view on calcium oscillations
title A statistical view on calcium oscillations
title_full A statistical view on calcium oscillations
title_fullStr A statistical view on calcium oscillations
title_full_unstemmed A statistical view on calcium oscillations
title_short A statistical view on calcium oscillations
title_sort statistical view on calcium oscillations
url https://eprints.nottingham.ac.uk/55055/