Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling
Purpose Descemet membrane endothelial keratoplasty (DMEK) and pre-Descemet endothelial keratoplasty (PDEK) tissues always scroll with the endothelial cells (EC) outside. We designed a study to understand the reason for this behavior. Design Experimental study. Methods Elastin content...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018
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| Online Access: | https://eprints.nottingham.ac.uk/55042/ |
| _version_ | 1848799106757033984 |
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| author | Mohammed, Imran Ross, Andrew R. Britton, John O. Said, Dalia G. Dua, Harminder S. |
| author_facet | Mohammed, Imran Ross, Andrew R. Britton, John O. Said, Dalia G. Dua, Harminder S. |
| author_sort | Mohammed, Imran |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Purpose
Descemet membrane endothelial keratoplasty (DMEK) and pre-Descemet endothelial keratoplasty (PDEK) tissues always scroll with the endothelial cells (EC) outside. We designed a study to understand the reason for this behavior.
Design
Experimental study.
Methods
Elastin content in Descemet membrane (DM), pre-Descemet layer (PDL), central and peripheral stroma, sclera, and trabecular meshwork were measured by the Fastin elastin assay kit. Distribution of elastin in DM, PDL, and anterior lens capsule (ALC) were examined by immunohistology. The effect of recombinant elastase enzyme and the effect of complete removal of EC and epithelial cells on the scrolling of DM and ALC, respectively, were studied.
Results
PDL showed the highest elastin content among the different tissues studied. Elastin localized as a distinct anterior band in the DM and was uniformly distributed in the PDL demarcating the latter from corneal stroma. Enzymatic treatment of DM with elastase reversed scrolling and corresponded with degradation or disappearance of elastin. Removal of EC did not affect the direction of scrolling. ALC behaved in the same manner with regard to distribution of elastin, scrolling, and removal of epithelial cells.
Conclusions
This pattern of elastin distribution in DM explains why DMEK and PDEK tissues always scroll with the EC outside. This behavior is not influenced by the EC. High elastin content and uniform distribution in the PDL suggest a structural difference from the posterior stroma. |
| first_indexed | 2025-11-14T20:30:24Z |
| format | Article |
| id | nottingham-55042 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:30:24Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-550422019-07-17T04:30:35Z https://eprints.nottingham.ac.uk/55042/ Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling Mohammed, Imran Ross, Andrew R. Britton, John O. Said, Dalia G. Dua, Harminder S. Purpose Descemet membrane endothelial keratoplasty (DMEK) and pre-Descemet endothelial keratoplasty (PDEK) tissues always scroll with the endothelial cells (EC) outside. We designed a study to understand the reason for this behavior. Design Experimental study. Methods Elastin content in Descemet membrane (DM), pre-Descemet layer (PDL), central and peripheral stroma, sclera, and trabecular meshwork were measured by the Fastin elastin assay kit. Distribution of elastin in DM, PDL, and anterior lens capsule (ALC) were examined by immunohistology. The effect of recombinant elastase enzyme and the effect of complete removal of EC and epithelial cells on the scrolling of DM and ALC, respectively, were studied. Results PDL showed the highest elastin content among the different tissues studied. Elastin localized as a distinct anterior band in the DM and was uniformly distributed in the PDL demarcating the latter from corneal stroma. Enzymatic treatment of DM with elastase reversed scrolling and corresponded with degradation or disappearance of elastin. Removal of EC did not affect the direction of scrolling. ALC behaved in the same manner with regard to distribution of elastin, scrolling, and removal of epithelial cells. Conclusions This pattern of elastin distribution in DM explains why DMEK and PDEK tissues always scroll with the EC outside. This behavior is not influenced by the EC. High elastin content and uniform distribution in the PDL suggest a structural difference from the posterior stroma. Elsevier 2018-10 Article PeerReviewed application/pdf en cc_by_nc_nd https://eprints.nottingham.ac.uk/55042/1/revised-AJO-18-524R1.pdf Mohammed, Imran, Ross, Andrew R., Britton, John O., Said, Dalia G. and Dua, Harminder S. (2018) Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling. American Journal of Ophthalmology, 194 . pp. 16-25. ISSN 1879-1891 http://dx.doi.org/10.1016/j.ajo.2018.07.001 10.1016/j.ajo.2018.07.001 10.1016/j.ajo.2018.07.001 10.1016/j.ajo.2018.07.001 |
| spellingShingle | Mohammed, Imran Ross, Andrew R. Britton, John O. Said, Dalia G. Dua, Harminder S. Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling |
| title | Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling |
| title_full | Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling |
| title_fullStr | Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling |
| title_full_unstemmed | Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling |
| title_short | Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling |
| title_sort | elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling |
| url | https://eprints.nottingham.ac.uk/55042/ https://eprints.nottingham.ac.uk/55042/ https://eprints.nottingham.ac.uk/55042/ |