Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells
We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of spontaneous Raman spectroscopy from trapped objects....
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Optical Society of America
2018
|
| Online Access: | https://eprints.nottingham.ac.uk/54426/ |
| _version_ | 1848799047270268928 |
|---|---|
| author | Sinjab, Faris Awuah, Dennis Gibson, Graham Padgett, Miles Ghaemmaghami, Amir M. Notingher, Ioan |
| author_facet | Sinjab, Faris Awuah, Dennis Gibson, Graham Padgett, Miles Ghaemmaghami, Amir M. Notingher, Ioan |
| author_sort | Sinjab, Faris |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of spontaneous Raman spectroscopy from trapped objects. Raman scattering from each laser focus is spatially filtered using reflective apertures on a digital micro-mirror device, which can be reconfigured with flexible patterns at video rate. We discuss operation of the instrument, and performance and viability considerations for biological measurements. We then demonstrate the capability of the instrument for fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems. |
| first_indexed | 2025-11-14T20:29:27Z |
| format | Article |
| id | nottingham-54426 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:29:27Z |
| publishDate | 2018 |
| publisher | Optical Society of America |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-544262018-09-12T12:51:47Z https://eprints.nottingham.ac.uk/54426/ Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells Sinjab, Faris Awuah, Dennis Gibson, Graham Padgett, Miles Ghaemmaghami, Amir M. Notingher, Ioan We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of spontaneous Raman spectroscopy from trapped objects. Raman scattering from each laser focus is spatially filtered using reflective apertures on a digital micro-mirror device, which can be reconfigured with flexible patterns at video rate. We discuss operation of the instrument, and performance and viability considerations for biological measurements. We then demonstrate the capability of the instrument for fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems. Optical Society of America 2018-07-30 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/54426/1/proofs.pdf Sinjab, Faris, Awuah, Dennis, Gibson, Graham, Padgett, Miles, Ghaemmaghami, Amir M. and Notingher, Ioan (2018) Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells. Optics Express . ISSN 1094-4087 (In Press) |
| spellingShingle | Sinjab, Faris Awuah, Dennis Gibson, Graham Padgett, Miles Ghaemmaghami, Amir M. Notingher, Ioan Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells |
| title | Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells |
| title_full | Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells |
| title_fullStr | Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells |
| title_full_unstemmed | Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells |
| title_short | Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells |
| title_sort | holographic optical trapping raman micro-spectroscopy for non-invasive measurement and manipulation of live cells |
| url | https://eprints.nottingham.ac.uk/54426/ |