Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm)
An innovative procedure for plant chloroplasts isolation has been proposed, which consists of juice extraction by physical fractionation from plant material and recovery of its chloroplast-rich fraction (CRF) by centrifugation. This simple method has been applied to pea vine haulm subjected to diffe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | https://eprints.nottingham.ac.uk/53440/ |
| _version_ | 1848798941665034240 |
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| author | Torcello-Gómez, Amelia Gedi, Mohamed A. Ibbett, Roger Nawaz Husain, Khatija Briars, Rhianna Gray, David |
| author_facet | Torcello-Gómez, Amelia Gedi, Mohamed A. Ibbett, Roger Nawaz Husain, Khatija Briars, Rhianna Gray, David |
| author_sort | Torcello-Gómez, Amelia |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | An innovative procedure for plant chloroplasts isolation has been proposed, which consists of juice extraction by physical fractionation from plant material and recovery of its chloroplast-rich fraction (CRF) by centrifugation. This simple method has been applied to pea vine haulm subjected to different post-harvest treatments: blanching, storage at different relative humidity values and fermentation. Additionally, freeze storage of the extracted juice was carried out. The macronutrient (total lipids, proteins, ash and carbohydrates) and micronutrient (fatty acids, chlorophylls, β-carotene, α-tocopherol and ascorbic acid) content and composition of the CRF have been determined. The CRF isolated from fresh pea vine haulm is a potential source of essential micronutrients (α-linolenic acid, β-carotene, α-tocopherol) and carbohydrates, whereas the post-harvest treatments trialled have a detrimental effect on the nutritional content. Industrial applications for the recovered nutritionally rich fraction, such as food supplement ingredient or animal feeding, are likely envisaged, while optimising the use of green haulm. |
| first_indexed | 2025-11-14T20:27:46Z |
| format | Article |
| id | nottingham-53440 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:27:46Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-534402019-08-06T04:30:11Z https://eprints.nottingham.ac.uk/53440/ Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm) Torcello-Gómez, Amelia Gedi, Mohamed A. Ibbett, Roger Nawaz Husain, Khatija Briars, Rhianna Gray, David An innovative procedure for plant chloroplasts isolation has been proposed, which consists of juice extraction by physical fractionation from plant material and recovery of its chloroplast-rich fraction (CRF) by centrifugation. This simple method has been applied to pea vine haulm subjected to different post-harvest treatments: blanching, storage at different relative humidity values and fermentation. Additionally, freeze storage of the extracted juice was carried out. The macronutrient (total lipids, proteins, ash and carbohydrates) and micronutrient (fatty acids, chlorophylls, β-carotene, α-tocopherol and ascorbic acid) content and composition of the CRF have been determined. The CRF isolated from fresh pea vine haulm is a potential source of essential micronutrients (α-linolenic acid, β-carotene, α-tocopherol) and carbohydrates, whereas the post-harvest treatments trialled have a detrimental effect on the nutritional content. Industrial applications for the recovered nutritionally rich fraction, such as food supplement ingredient or animal feeding, are likely envisaged, while optimising the use of green haulm. Elsevier 2019-01-30 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/53440/1/Manuscript_pea%20plant%20waste_Torcello%20et%20al_Food%20Chem_revised.pdf Torcello-Gómez, Amelia, Gedi, Mohamed A., Ibbett, Roger, Nawaz Husain, Khatija, Briars, Rhianna and Gray, David (2019) Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm). Food Chemistry, 272 . pp. 18-25. ISSN 0308-8146 https://www.sciencedirect.com/science/article/pii/S0308814618314080?via%3Dihub doi:10.1016/j.foodchem.2018.08.018 doi:10.1016/j.foodchem.2018.08.018 |
| spellingShingle | Torcello-Gómez, Amelia Gedi, Mohamed A. Ibbett, Roger Nawaz Husain, Khatija Briars, Rhianna Gray, David Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm) |
| title | Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm) |
| title_full | Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm) |
| title_fullStr | Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm) |
| title_full_unstemmed | Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm) |
| title_short | Chloroplast-rich material from the physical fractionation of pea vine ( Pisum sativum ) postharvest field residue (Haulm) |
| title_sort | chloroplast-rich material from the physical fractionation of pea vine ( pisum sativum ) postharvest field residue (haulm) |
| url | https://eprints.nottingham.ac.uk/53440/ https://eprints.nottingham.ac.uk/53440/ https://eprints.nottingham.ac.uk/53440/ |