Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation
Brown adipose tissue (BAT) undergoes pronounced changes after birth coincident with the loss of the BAT-specifc uncoupling protein (UCP)1 and rapid fat growth. The extent to which this adaptation may vary between anatomical locations remains unknown, or whether the process is sensitive to maternal d...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2018
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| Online Access: | https://eprints.nottingham.ac.uk/53397/ |
| _version_ | 1848798932582268928 |
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| author | Fainberg, Hernan P. Birtwistle, Mark Alagal, Reham Alhaddad, Ahmad Pope, Mark Davies, Graeme Woods, Rachel Castellanos, Marcos May, Sean T. Ortori, Catharine A. Barrett, David A. Perry, Viv Wiens, Frank Stahl, Bernd van der Beek, Eline Sacks, Harold Budge, Helen Symonds, Michael E. |
| author_facet | Fainberg, Hernan P. Birtwistle, Mark Alagal, Reham Alhaddad, Ahmad Pope, Mark Davies, Graeme Woods, Rachel Castellanos, Marcos May, Sean T. Ortori, Catharine A. Barrett, David A. Perry, Viv Wiens, Frank Stahl, Bernd van der Beek, Eline Sacks, Harold Budge, Helen Symonds, Michael E. |
| author_sort | Fainberg, Hernan P. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Brown adipose tissue (BAT) undergoes pronounced changes after birth coincident with the loss of the BAT-specifc uncoupling protein (UCP)1 and rapid fat growth. The extent to which this adaptation may vary between anatomical locations remains unknown, or whether the process is sensitive to maternal dietary supplementation. We, therefore, conducted a data mining based study on the major fat depots (i.e. epicardial, perirenal, sternal (which possess UCP1 at 7 days), subcutaneous and omental) (that do not possess UCP1) of young sheep during the frst month of life. Initially we determined what effect adding 3% canola oil to the maternal diet has on mitochondrial protein abundance in those depots which possessed UCP1. This demonstrated that maternal dietary supplementation delayed the loss of mitochondrial proteins, with the amount of cytochrome C actually being increased. Using machine learning algorithms followed by weighted gene co-expression network analysis, we demonstrated that each depot could be segregated into a unique and concise set of modules containing co-expressed genes involved in adipose function. Finally using lipidomic analysis following the maternal dietary intervention, we confrmed the perirenal depot to be most responsive. These insights point at new research avenues for examining interventions to modulate fat development in early life. |
| first_indexed | 2025-11-14T20:27:38Z |
| format | Article |
| id | nottingham-53397 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:27:38Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-533972018-08-28T08:55:10Z https://eprints.nottingham.ac.uk/53397/ Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation Fainberg, Hernan P. Birtwistle, Mark Alagal, Reham Alhaddad, Ahmad Pope, Mark Davies, Graeme Woods, Rachel Castellanos, Marcos May, Sean T. Ortori, Catharine A. Barrett, David A. Perry, Viv Wiens, Frank Stahl, Bernd van der Beek, Eline Sacks, Harold Budge, Helen Symonds, Michael E. Brown adipose tissue (BAT) undergoes pronounced changes after birth coincident with the loss of the BAT-specifc uncoupling protein (UCP)1 and rapid fat growth. The extent to which this adaptation may vary between anatomical locations remains unknown, or whether the process is sensitive to maternal dietary supplementation. We, therefore, conducted a data mining based study on the major fat depots (i.e. epicardial, perirenal, sternal (which possess UCP1 at 7 days), subcutaneous and omental) (that do not possess UCP1) of young sheep during the frst month of life. Initially we determined what effect adding 3% canola oil to the maternal diet has on mitochondrial protein abundance in those depots which possessed UCP1. This demonstrated that maternal dietary supplementation delayed the loss of mitochondrial proteins, with the amount of cytochrome C actually being increased. Using machine learning algorithms followed by weighted gene co-expression network analysis, we demonstrated that each depot could be segregated into a unique and concise set of modules containing co-expressed genes involved in adipose function. Finally using lipidomic analysis following the maternal dietary intervention, we confrmed the perirenal depot to be most responsive. These insights point at new research avenues for examining interventions to modulate fat development in early life. Nature Publishing Group 2018-06-25 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/53397/1/s41598-018-27376-3.pdf Fainberg, Hernan P., Birtwistle, Mark, Alagal, Reham, Alhaddad, Ahmad, Pope, Mark, Davies, Graeme, Woods, Rachel, Castellanos, Marcos, May, Sean T., Ortori, Catharine A., Barrett, David A., Perry, Viv, Wiens, Frank, Stahl, Bernd, van der Beek, Eline, Sacks, Harold, Budge, Helen and Symonds, Michael E. (2018) Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation. Scientific Reports, 8 (1). 96258/1-96258/11. ISSN 2045-2322 https://www.nature.com/articles/s41598-018-27376-3 doi:10.1038/s41598-018-27376-3 doi:10.1038/s41598-018-27376-3 |
| spellingShingle | Fainberg, Hernan P. Birtwistle, Mark Alagal, Reham Alhaddad, Ahmad Pope, Mark Davies, Graeme Woods, Rachel Castellanos, Marcos May, Sean T. Ortori, Catharine A. Barrett, David A. Perry, Viv Wiens, Frank Stahl, Bernd van der Beek, Eline Sacks, Harold Budge, Helen Symonds, Michael E. Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation |
| title | Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation |
| title_full | Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation |
| title_fullStr | Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation |
| title_full_unstemmed | Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation |
| title_short | Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation |
| title_sort | transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation |
| url | https://eprints.nottingham.ac.uk/53397/ https://eprints.nottingham.ac.uk/53397/ https://eprints.nottingham.ac.uk/53397/ |