Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta
The AnxA2/S100A10 complex has been implicated in various placental functions but although the localisation of these proteins individually has been studied, there is no information about the localisation of their complex in situ at the cellular level. Using the proximity ligation technique, we have i...
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| Format: | Article |
| Language: | English |
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Springer
2018
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| Online Access: | https://eprints.nottingham.ac.uk/53385/ |
| _version_ | 1848798930082463744 |
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| author | Abd El-Aleem, Seham Dekker, Lodewijk V. |
| author_facet | Abd El-Aleem, Seham Dekker, Lodewijk V. |
| author_sort | Abd El-Aleem, Seham |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The AnxA2/S100A10 complex has been implicated in various placental functions but although the localisation of these proteins individually has been studied, there is no information about the localisation of their complex in situ at the cellular level. Using the proximity ligation technique, we have investigated the in situ localisation of AnxA2/S100A10 complex in the placenta and have compared this with the location patterns of the individual proteins. High levels of expression of AnxA2/S100A10 complexes were observed in the amniotic membrane and in blood vessel endothelial cells. Lower levels were detected in the brush border area of the syncytium and in the trophoblasts. Immunohistochemical analysis of AnxA2 and S100A10 individually revealed broadly similar patterns of localisation. The brush border staining pattern suggests that in this location at least some of the AnxA2 is not in complex with S100A10. The formal location of the AnxA2/S100A10 complex is compatible with a role in cell-cell interaction, intracellular transport and secretory processes and regulation of cell surface proteases, implying contributions to membrane integrity, nutrient exchange, placentation and vascular remodelling in different parts of the placenta. Future applications will allow specific assessment of the association of the complex with pathophysiological disorders. |
| first_indexed | 2025-11-14T20:27:35Z |
| format | Article |
| id | nottingham-53385 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:27:35Z |
| publishDate | 2018 |
| publisher | Springer |
| recordtype | eprints |
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| spelling | nottingham-533852019-08-22T04:30:26Z https://eprints.nottingham.ac.uk/53385/ Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta Abd El-Aleem, Seham Dekker, Lodewijk V. The AnxA2/S100A10 complex has been implicated in various placental functions but although the localisation of these proteins individually has been studied, there is no information about the localisation of their complex in situ at the cellular level. Using the proximity ligation technique, we have investigated the in situ localisation of AnxA2/S100A10 complex in the placenta and have compared this with the location patterns of the individual proteins. High levels of expression of AnxA2/S100A10 complexes were observed in the amniotic membrane and in blood vessel endothelial cells. Lower levels were detected in the brush border area of the syncytium and in the trophoblasts. Immunohistochemical analysis of AnxA2 and S100A10 individually revealed broadly similar patterns of localisation. The brush border staining pattern suggests that in this location at least some of the AnxA2 is not in complex with S100A10. The formal location of the AnxA2/S100A10 complex is compatible with a role in cell-cell interaction, intracellular transport and secretory processes and regulation of cell surface proteases, implying contributions to membrane integrity, nutrient exchange, placentation and vascular remodelling in different parts of the placenta. Future applications will allow specific assessment of the association of the complex with pathophysiological disorders. Springer 2018-07-30 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/53385/1/Abd%20El-Aleem%20and%20Dekker%202018.pdf Abd El-Aleem, Seham and Dekker, Lodewijk V. (2018) Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta. Journal of Molecular HIstology . ISSN 1567-2387 (In Press) |
| spellingShingle | Abd El-Aleem, Seham Dekker, Lodewijk V. Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta |
| title | Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta |
| title_full | Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta |
| title_fullStr | Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta |
| title_full_unstemmed | Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta |
| title_short | Assessment of the cellular localisation of the Annexin A2/S100A10 complex in human placenta |
| title_sort | assessment of the cellular localisation of the annexin a2/s100a10 complex in human placenta |
| url | https://eprints.nottingham.ac.uk/53385/ |