AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling
Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membran...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52988/ |
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| author | Dindas, Julian Scherzer, Sönke Roelfsema, M. Rob G. von Meyer, Katharina Müller, Heike M. Al-Rasheid, K.A.S. Palme, Klaus Dietrich, Petra Becker, Dirk Bennett, Malcolm J. Hedrich, Rainer |
| author_facet | Dindas, Julian Scherzer, Sönke Roelfsema, M. Rob G. von Meyer, Katharina Müller, Heike M. Al-Rasheid, K.A.S. Palme, Klaus Dietrich, Petra Becker, Dirk Bennett, Malcolm J. Hedrich, Rainer |
| author_sort | Dindas, Julian |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membrane depolarization, in response to IAA in a concentration- and pH-dependent manner. This depolarization is strongly impaired in aux1 mutants, indicating that AUX1 is the major transporter for auxin uptake in root hairs. Local intracellular auxin application triggers Ca2+ signals that propagate as long-distance waves between root cells and modulate their auxin responses. AUX1-mediated IAA transport, as well as IAA- triggered calcium signals, are blocked by treatment with the SCFTIR1/AFB - inhibitor auxinole. Further, they are strongly reduced in the tir1afb2afb3 and the cngc14 mutant. Our study reveals that the AUX1 transporter, the SCFTIR1/AFB receptor and the CNGC14 Ca2+ channel, mediate fast auxin signaling in roots. |
| first_indexed | 2025-11-14T20:26:24Z |
| format | Article |
| id | nottingham-52988 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:26:24Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-529882020-05-08T08:02:33Z https://eprints.nottingham.ac.uk/52988/ AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling Dindas, Julian Scherzer, Sönke Roelfsema, M. Rob G. von Meyer, Katharina Müller, Heike M. Al-Rasheid, K.A.S. Palme, Klaus Dietrich, Petra Becker, Dirk Bennett, Malcolm J. Hedrich, Rainer Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membrane depolarization, in response to IAA in a concentration- and pH-dependent manner. This depolarization is strongly impaired in aux1 mutants, indicating that AUX1 is the major transporter for auxin uptake in root hairs. Local intracellular auxin application triggers Ca2+ signals that propagate as long-distance waves between root cells and modulate their auxin responses. AUX1-mediated IAA transport, as well as IAA- triggered calcium signals, are blocked by treatment with the SCFTIR1/AFB - inhibitor auxinole. Further, they are strongly reduced in the tir1afb2afb3 and the cngc14 mutant. Our study reveals that the AUX1 transporter, the SCFTIR1/AFB receptor and the CNGC14 Ca2+ channel, mediate fast auxin signaling in roots. Nature Publishing Group 2018-03-21 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/52988/1/s41467-018-03582-5.pdf Dindas, Julian, Scherzer, Sönke, Roelfsema, M. Rob G., von Meyer, Katharina, Müller, Heike M., Al-Rasheid, K.A.S., Palme, Klaus, Dietrich, Petra, Becker, Dirk, Bennett, Malcolm J. and Hedrich, Rainer (2018) AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling. Nature Communications, 9 (1). 1174/1-1174/10. ISSN 2041-1723 https://www.nature.com/articles/s41467-018-03582-5 doi:10.1038/s41467-018-03582-5 doi:10.1038/s41467-018-03582-5 |
| spellingShingle | Dindas, Julian Scherzer, Sönke Roelfsema, M. Rob G. von Meyer, Katharina Müller, Heike M. Al-Rasheid, K.A.S. Palme, Klaus Dietrich, Petra Becker, Dirk Bennett, Malcolm J. Hedrich, Rainer AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling |
| title | AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling |
| title_full | AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling |
| title_fullStr | AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling |
| title_full_unstemmed | AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling |
| title_short | AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling |
| title_sort | aux1-mediated root hair auxin influx governs scftir1/afb-type ca2+ signaling |
| url | https://eprints.nottingham.ac.uk/52988/ https://eprints.nottingham.ac.uk/52988/ https://eprints.nottingham.ac.uk/52988/ |