Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system

The intestinal lymphatic system plays an important role in the pathophysiology of multiple diseases including lymphomas, cancer metastasis, autoimmune diseases, and human immunodeficiency virus (HIV) infection. It is thus an important compartment for delivery of drugs in order to treat diseases asso...

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Main Authors: Lee, Jong Bong, Zgair, Atheer, Malec, Jed, Kim, Tae Hwan, Kim, Min Gi, Ali, Joseph, Qin, Chaolong, Feng, Wanshan, Chiang, Manting, Gao, Xizhe, Voronin, Gregory, Garces, Aimie, Lau, Chun Long, Chan, Ting-Hoi, Hume, Amy, McIntosh, Tecashanell M., Soukarieh, Fadi, Al-Hayali, Mohammed, Cipolla, Elena, Collins, Hilary M., Heery, David M., Shin, Beom Soo, Yoo, Sun Dong, Kagan, Leonid, Stocks, Michael J., Bradshaw, Tracey D., Fischer, Peter M., Gershkovich, Pavel
Format: Article
Published: Elsevier 2018
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Online Access:https://eprints.nottingham.ac.uk/52980/
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author Lee, Jong Bong
Zgair, Atheer
Malec, Jed
Kim, Tae Hwan
Kim, Min Gi
Ali, Joseph
Qin, Chaolong
Feng, Wanshan
Chiang, Manting
Gao, Xizhe
Voronin, Gregory
Garces, Aimie
Lau, Chun Long
Chan, Ting-Hoi
Hume, Amy
McIntosh, Tecashanell M.
Soukarieh, Fadi
Al-Hayali, Mohammed
Cipolla, Elena
Collins, Hilary M.
Heery, David M.
Shin, Beom Soo
Yoo, Sun Dong
Kagan, Leonid
Stocks, Michael J.
Bradshaw, Tracey D.
Fischer, Peter M.
Gershkovich, Pavel
author_facet Lee, Jong Bong
Zgair, Atheer
Malec, Jed
Kim, Tae Hwan
Kim, Min Gi
Ali, Joseph
Qin, Chaolong
Feng, Wanshan
Chiang, Manting
Gao, Xizhe
Voronin, Gregory
Garces, Aimie
Lau, Chun Long
Chan, Ting-Hoi
Hume, Amy
McIntosh, Tecashanell M.
Soukarieh, Fadi
Al-Hayali, Mohammed
Cipolla, Elena
Collins, Hilary M.
Heery, David M.
Shin, Beom Soo
Yoo, Sun Dong
Kagan, Leonid
Stocks, Michael J.
Bradshaw, Tracey D.
Fischer, Peter M.
Gershkovich, Pavel
author_sort Lee, Jong Bong
building Nottingham Research Data Repository
collection Online Access
description The intestinal lymphatic system plays an important role in the pathophysiology of multiple diseases including lymphomas, cancer metastasis, autoimmune diseases, and human immunodeficiency virus (HIV) infection. It is thus an important compartment for delivery of drugs in order to treat diseases associated with the lymphatic system. Lipophilic prodrug approaches have been used in the past to take advantage of the intestinal lymphatic transport processes to deliver drugs to the intestinal lymphatics. Most of the approaches previously adopted were based on very bulky prodrug moieties such as those mimicking triglycerides (TG). We now report a study in which a lipophilic prodrug approach was used to efficiently deliver bexarotene (BEX) and retinoic acid (RA) to the intestinal lymphatic system using activated ester prodrugs. A range of carboxylic ester prodrugs of BEX were designed and synthesised and all of the esters showed improved association with chylomicrons, which indicated an improved potential for delivery to the intestinal lymphatic system. The conversion rate of the prodrugs to BEX was the main determinant in delivery of BEX to the intestinal lymphatics, and activated ester prodrugs were prepared to enhance the conversion rate. As a result, an 4-(hydroxymethyl)-1,3-dioxol-2-one ester prodrug of BEX was able to increase the exposure of the mesenteric lymph nodes (MLNs) to BEX 17-fold compared to when BEX itself was administered. The activated ester prodrug approach was also applied to another drug, RA, where the exposure of the MLNs was increased 2.4-fold through the application of a similar cyclic activated prodrug. Synergism between BEX and RA was also demonstrated in vitro by cell growth inhibition assays using lymphoma cell lines. In conclusion, the activated ester prodrug approach results in efficient delivery of drugs to the intestinal lymphatic system, which could benefit patients affected by a large number of pathological conditions.
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spelling nottingham-529802020-05-04T19:49:18Z https://eprints.nottingham.ac.uk/52980/ Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system Lee, Jong Bong Zgair, Atheer Malec, Jed Kim, Tae Hwan Kim, Min Gi Ali, Joseph Qin, Chaolong Feng, Wanshan Chiang, Manting Gao, Xizhe Voronin, Gregory Garces, Aimie Lau, Chun Long Chan, Ting-Hoi Hume, Amy McIntosh, Tecashanell M. Soukarieh, Fadi Al-Hayali, Mohammed Cipolla, Elena Collins, Hilary M. Heery, David M. Shin, Beom Soo Yoo, Sun Dong Kagan, Leonid Stocks, Michael J. Bradshaw, Tracey D. Fischer, Peter M. Gershkovich, Pavel The intestinal lymphatic system plays an important role in the pathophysiology of multiple diseases including lymphomas, cancer metastasis, autoimmune diseases, and human immunodeficiency virus (HIV) infection. It is thus an important compartment for delivery of drugs in order to treat diseases associated with the lymphatic system. Lipophilic prodrug approaches have been used in the past to take advantage of the intestinal lymphatic transport processes to deliver drugs to the intestinal lymphatics. Most of the approaches previously adopted were based on very bulky prodrug moieties such as those mimicking triglycerides (TG). We now report a study in which a lipophilic prodrug approach was used to efficiently deliver bexarotene (BEX) and retinoic acid (RA) to the intestinal lymphatic system using activated ester prodrugs. A range of carboxylic ester prodrugs of BEX were designed and synthesised and all of the esters showed improved association with chylomicrons, which indicated an improved potential for delivery to the intestinal lymphatic system. The conversion rate of the prodrugs to BEX was the main determinant in delivery of BEX to the intestinal lymphatics, and activated ester prodrugs were prepared to enhance the conversion rate. As a result, an 4-(hydroxymethyl)-1,3-dioxol-2-one ester prodrug of BEX was able to increase the exposure of the mesenteric lymph nodes (MLNs) to BEX 17-fold compared to when BEX itself was administered. The activated ester prodrug approach was also applied to another drug, RA, where the exposure of the MLNs was increased 2.4-fold through the application of a similar cyclic activated prodrug. Synergism between BEX and RA was also demonstrated in vitro by cell growth inhibition assays using lymphoma cell lines. In conclusion, the activated ester prodrug approach results in efficient delivery of drugs to the intestinal lymphatic system, which could benefit patients affected by a large number of pathological conditions. Elsevier 2018-09-28 Article PeerReviewed Lee, Jong Bong, Zgair, Atheer, Malec, Jed, Kim, Tae Hwan, Kim, Min Gi, Ali, Joseph, Qin, Chaolong, Feng, Wanshan, Chiang, Manting, Gao, Xizhe, Voronin, Gregory, Garces, Aimie, Lau, Chun Long, Chan, Ting-Hoi, Hume, Amy, McIntosh, Tecashanell M., Soukarieh, Fadi, Al-Hayali, Mohammed, Cipolla, Elena, Collins, Hilary M., Heery, David M., Shin, Beom Soo, Yoo, Sun Dong, Kagan, Leonid, Stocks, Michael J., Bradshaw, Tracey D., Fischer, Peter M. and Gershkovich, Pavel (2018) Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system. Journal of Controlled Release, 286 . pp. 10-19. ISSN 1873-4995 Lymphatic transport; Prodrugs; Bexarotene; Retinoic acid; Chylomicron; Activated esters https://www.sciencedirect.com/science/article/pii/S0168365918304164 doi:10.1016/j.jconrel.2018.07.022 doi:10.1016/j.jconrel.2018.07.022
spellingShingle Lymphatic transport; Prodrugs; Bexarotene; Retinoic acid; Chylomicron; Activated esters
Lee, Jong Bong
Zgair, Atheer
Malec, Jed
Kim, Tae Hwan
Kim, Min Gi
Ali, Joseph
Qin, Chaolong
Feng, Wanshan
Chiang, Manting
Gao, Xizhe
Voronin, Gregory
Garces, Aimie
Lau, Chun Long
Chan, Ting-Hoi
Hume, Amy
McIntosh, Tecashanell M.
Soukarieh, Fadi
Al-Hayali, Mohammed
Cipolla, Elena
Collins, Hilary M.
Heery, David M.
Shin, Beom Soo
Yoo, Sun Dong
Kagan, Leonid
Stocks, Michael J.
Bradshaw, Tracey D.
Fischer, Peter M.
Gershkovich, Pavel
Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system
title Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system
title_full Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system
title_fullStr Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system
title_full_unstemmed Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system
title_short Lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system
title_sort lipophilic activated ester prodrug approach for drug delivery to the intestinal lymphatic system
topic Lymphatic transport; Prodrugs; Bexarotene; Retinoic acid; Chylomicron; Activated esters
url https://eprints.nottingham.ac.uk/52980/
https://eprints.nottingham.ac.uk/52980/
https://eprints.nottingham.ac.uk/52980/