Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis
Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis, is a highly regulated process but the detailed mechanism still remains inc...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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American Society for Biochemistry and Molecular Biology
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52976/ |
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| author | Chung, Gary Hong Chun Domart, Marie-Charlotte Peddie, Christopher Mantell, Judith Mclaverty, Kieran Arabiotorre, Angela Hodgson, Lorna Byrne, Richard D. Verkade, Paul Arkill, Kenton P. Collinson, Lucy M. Larijani, Banafshé |
| author_facet | Chung, Gary Hong Chun Domart, Marie-Charlotte Peddie, Christopher Mantell, Judith Mclaverty, Kieran Arabiotorre, Angela Hodgson, Lorna Byrne, Richard D. Verkade, Paul Arkill, Kenton P. Collinson, Lucy M. Larijani, Banafshé |
| author_sort | Chung, Gary Hong Chun |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis, is a highly regulated process but the detailed mechanism still remains incompletely understood. Previous studies have found isolated diacylglycerol (DAG) containing vesicles are essential for completing the fusion of NE in non-somatic cells. We investigated the impact of DAG depletion from cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a miniSOG-C1-domain tag that localized DAG-enriched vesicles at the perinuclear region, which suggested the existence of NE fusogenic vesicles. We quantified the impact of Golgi-DAG depletion by measuring the in situ NE rim curvature of the re-forming NE. The rim curvature in these cells was significantly reduced compared with controls, which indicated a localized defect in NE morphology. Our novel results demonstrate the significance of the role of DAG from cis-Golgi for the regulation of NE assembly. |
| first_indexed | 2025-11-14T20:26:20Z |
| format | Article |
| id | nottingham-52976 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:26:20Z |
| publishDate | 2018 |
| publisher | American Society for Biochemistry and Molecular Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-529762020-05-04T19:40:30Z https://eprints.nottingham.ac.uk/52976/ Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis Chung, Gary Hong Chun Domart, Marie-Charlotte Peddie, Christopher Mantell, Judith Mclaverty, Kieran Arabiotorre, Angela Hodgson, Lorna Byrne, Richard D. Verkade, Paul Arkill, Kenton P. Collinson, Lucy M. Larijani, Banafshé Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis, is a highly regulated process but the detailed mechanism still remains incompletely understood. Previous studies have found isolated diacylglycerol (DAG) containing vesicles are essential for completing the fusion of NE in non-somatic cells. We investigated the impact of DAG depletion from cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a miniSOG-C1-domain tag that localized DAG-enriched vesicles at the perinuclear region, which suggested the existence of NE fusogenic vesicles. We quantified the impact of Golgi-DAG depletion by measuring the in situ NE rim curvature of the re-forming NE. The rim curvature in these cells was significantly reduced compared with controls, which indicated a localized defect in NE morphology. Our novel results demonstrate the significance of the role of DAG from cis-Golgi for the regulation of NE assembly. American Society for Biochemistry and Molecular Biology 2018-06-12 Article PeerReviewed Chung, Gary Hong Chun, Domart, Marie-Charlotte, Peddie, Christopher, Mantell, Judith, Mclaverty, Kieran, Arabiotorre, Angela, Hodgson, Lorna, Byrne, Richard D., Verkade, Paul, Arkill, Kenton P., Collinson, Lucy M. and Larijani, Banafshé (2018) Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis. Journal of Lipid Research . ISSN 1539-7262 Cancer; Cell signaling; Diacylglycerol; Electron microscopy; Membranes/fusion; Nuclear envelope formation; Open mitosis http://dx.doi.org/10.1194/jlr.M083899 doi:10.1194/jlr.M083899 doi:10.1194/jlr.M083899 |
| spellingShingle | Cancer; Cell signaling; Diacylglycerol; Electron microscopy; Membranes/fusion; Nuclear envelope formation; Open mitosis Chung, Gary Hong Chun Domart, Marie-Charlotte Peddie, Christopher Mantell, Judith Mclaverty, Kieran Arabiotorre, Angela Hodgson, Lorna Byrne, Richard D. Verkade, Paul Arkill, Kenton P. Collinson, Lucy M. Larijani, Banafshé Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis |
| title | Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis |
| title_full | Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis |
| title_fullStr | Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis |
| title_full_unstemmed | Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis |
| title_short | Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis |
| title_sort | acute depletion of diacylglycerol from the cis-golgi affects localised nuclear envelope morphology during mitosis |
| topic | Cancer; Cell signaling; Diacylglycerol; Electron microscopy; Membranes/fusion; Nuclear envelope formation; Open mitosis |
| url | https://eprints.nottingham.ac.uk/52976/ https://eprints.nottingham.ac.uk/52976/ https://eprints.nottingham.ac.uk/52976/ |