Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy
Photodynamic therapy (PDT) is a treatment of cancer by which tumour cells are destroyed using reactive oxygen species produced by photosensitizers following activation with visible or near infrared light. Successful PDT depends on the solubility and the targeting ability of the photosensitizers. In...
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/52917/ |
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| author | Penon, Oriol Marín, María J. Russell, David A. Pérez-García, Lluïsa |
| author_facet | Penon, Oriol Marín, María J. Russell, David A. Pérez-García, Lluïsa |
| author_sort | Penon, Oriol |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Photodynamic therapy (PDT) is a treatment of cancer by which tumour cells are destroyed using reactive oxygen species produced by photosensitizers following activation with visible or near infrared light. Successful PDT depends on the solubility and the targeting ability of the photosensitizers. In this work, the synthesis of a porphyrin-based water soluble nanoparticle conjugate containing a targeting agent that recognizes the erbB2 receptor overexpressed on the surface of particular cancer cells is reported. The nanoparticle conjugates were synthesized following two different protocols, viz. a biphasic and a monophasic method, with the aim to determine which method yielded the optimal nanosystem for potential PDT applications. The nanoparticles were characterized using UV–Vis absorption and fluorescence spectroscopies together with transmission electron microscopy and zeta potential measurements; and their ability to produce singlet oxygen following irradiation was investigated following the decay in absorption of a singlet oxygen probe. The nanoparticles synthesized using the monophasic method were shown to produce the highest amount of singlet oxygen and were further functionalized with anti-erbB2 antibody to target the erbB2 receptors expressed on the surface of SK-BR-3 human breast cancer cells. The water soluble, antibody-porphyrin nanoparticle conjugates were shown to elicit targeted PDT of the breast cancer cells. |
| first_indexed | 2025-11-14T20:26:09Z |
| format | Article |
| id | nottingham-52917 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:26:09Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
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| spelling | nottingham-529172020-05-04T18:50:05Z https://eprints.nottingham.ac.uk/52917/ Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy Penon, Oriol Marín, María J. Russell, David A. Pérez-García, Lluïsa Photodynamic therapy (PDT) is a treatment of cancer by which tumour cells are destroyed using reactive oxygen species produced by photosensitizers following activation with visible or near infrared light. Successful PDT depends on the solubility and the targeting ability of the photosensitizers. In this work, the synthesis of a porphyrin-based water soluble nanoparticle conjugate containing a targeting agent that recognizes the erbB2 receptor overexpressed on the surface of particular cancer cells is reported. The nanoparticle conjugates were synthesized following two different protocols, viz. a biphasic and a monophasic method, with the aim to determine which method yielded the optimal nanosystem for potential PDT applications. The nanoparticles were characterized using UV–Vis absorption and fluorescence spectroscopies together with transmission electron microscopy and zeta potential measurements; and their ability to produce singlet oxygen following irradiation was investigated following the decay in absorption of a singlet oxygen probe. The nanoparticles synthesized using the monophasic method were shown to produce the highest amount of singlet oxygen and were further functionalized with anti-erbB2 antibody to target the erbB2 receptors expressed on the surface of SK-BR-3 human breast cancer cells. The water soluble, antibody-porphyrin nanoparticle conjugates were shown to elicit targeted PDT of the breast cancer cells. Elsevier 2017-06-15 Article PeerReviewed Penon, Oriol, Marín, María J., Russell, David A. and Pérez-García, Lluïsa (2017) Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy. Journal of Colloid and Interface Science, 496 . pp. 100-110. ISSN 1095-7103 Photodynamic therapy ; Multifunctional nanoparticles ; Porphyrins ; Gold nanoparticles (AuNPs) ; Water soluble nanoconjugates ; Targeting antibodies ; Singlet oxygen production ; In vitro phototoxicity ; Human breast cancer cells https://www.sciencedirect.com/science/article/pii/S002197971730156X?via%3Dihub doi:10.1016/j.jcis.2017.02.006 doi:10.1016/j.jcis.2017.02.006 |
| spellingShingle | Photodynamic therapy ; Multifunctional nanoparticles ; Porphyrins ; Gold nanoparticles (AuNPs) ; Water soluble nanoconjugates ; Targeting antibodies ; Singlet oxygen production ; In vitro phototoxicity ; Human breast cancer cells Penon, Oriol Marín, María J. Russell, David A. Pérez-García, Lluïsa Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy |
| title | Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy |
| title_full | Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy |
| title_fullStr | Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy |
| title_full_unstemmed | Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy |
| title_short | Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy |
| title_sort | water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy |
| topic | Photodynamic therapy ; Multifunctional nanoparticles ; Porphyrins ; Gold nanoparticles (AuNPs) ; Water soluble nanoconjugates ; Targeting antibodies ; Singlet oxygen production ; In vitro phototoxicity ; Human breast cancer cells |
| url | https://eprints.nottingham.ac.uk/52917/ https://eprints.nottingham.ac.uk/52917/ https://eprints.nottingham.ac.uk/52917/ |