Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications

The development of micro- and nanosystems for their use in biomedicine is a continuously growing field. One of the major goals of such platforms is to combine multiple functions in a single entity. However, achieving the design of an efficient and safe micro- or nanoplatform has shown to be strongly...

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Main Authors: Patiño, Tania, Soriano, Jorge, Amirthalingam, Ezhil, Duran, Sara, González-Campo, Arántzazu, Duch, Marta, Ibáñez, Elena, Barrios, Leonardo, Plaza, Jose Antonio, Pérez-García, Lluïsa, Nogués, Carme
Format: Article
Published: Royal Society of Chemistry 2016
Online Access:https://eprints.nottingham.ac.uk/52914/
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author Patiño, Tania
Soriano, Jorge
Amirthalingam, Ezhil
Duran, Sara
González-Campo, Arántzazu
Duch, Marta
Ibáñez, Elena
Barrios, Leonardo
Plaza, Jose Antonio
Pérez-García, Lluïsa
Nogués, Carme
author_facet Patiño, Tania
Soriano, Jorge
Amirthalingam, Ezhil
Duran, Sara
González-Campo, Arántzazu
Duch, Marta
Ibáñez, Elena
Barrios, Leonardo
Plaza, Jose Antonio
Pérez-García, Lluïsa
Nogués, Carme
author_sort Patiño, Tania
building Nottingham Research Data Repository
collection Online Access
description The development of micro- and nanosystems for their use in biomedicine is a continuously growing field. One of the major goals of such platforms is to combine multiple functions in a single entity. However, achieving the design of an efficient and safe micro- or nanoplatform has shown to be strongly influenced by its interaction with the biological systems, where particle features or cell types play a critical role. In this work, the feasibility of using multi-material pSi-Cr-Au intracellular chips (MMICCs) for multifunctional applications by characterizing their interactions with two different cell lines, one tumorigenic and one non-tumorigenic, in terms of biocompatibility, internalization and intracellular fate, has been explored. Moreover, the impact of MMICCs on the induction of an inflammatory response has been assessed by evaluating TNFα, IL1b, IL6, and IL10 human inflammatory cytokines secretion by macrophages. Results show that MMICCs are biocompatible and their internalization efficiency is strongly dependent on the cell type. Finally as a proof-of-concept, MMICCs have been dually functionalized with transferrin and pHrodo™ Red, SE to target cancer cells and detect intracellular pH, respectively. In conclusion, MMICCs can be used as multi-functional devices due to their high biocompatibility, non-inflammatory properties and the ability of developing multiple functions.
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spelling nottingham-529142020-05-04T17:47:10Z https://eprints.nottingham.ac.uk/52914/ Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications Patiño, Tania Soriano, Jorge Amirthalingam, Ezhil Duran, Sara González-Campo, Arántzazu Duch, Marta Ibáñez, Elena Barrios, Leonardo Plaza, Jose Antonio Pérez-García, Lluïsa Nogués, Carme The development of micro- and nanosystems for their use in biomedicine is a continuously growing field. One of the major goals of such platforms is to combine multiple functions in a single entity. However, achieving the design of an efficient and safe micro- or nanoplatform has shown to be strongly influenced by its interaction with the biological systems, where particle features or cell types play a critical role. In this work, the feasibility of using multi-material pSi-Cr-Au intracellular chips (MMICCs) for multifunctional applications by characterizing their interactions with two different cell lines, one tumorigenic and one non-tumorigenic, in terms of biocompatibility, internalization and intracellular fate, has been explored. Moreover, the impact of MMICCs on the induction of an inflammatory response has been assessed by evaluating TNFα, IL1b, IL6, and IL10 human inflammatory cytokines secretion by macrophages. Results show that MMICCs are biocompatible and their internalization efficiency is strongly dependent on the cell type. Finally as a proof-of-concept, MMICCs have been dually functionalized with transferrin and pHrodo™ Red, SE to target cancer cells and detect intracellular pH, respectively. In conclusion, MMICCs can be used as multi-functional devices due to their high biocompatibility, non-inflammatory properties and the ability of developing multiple functions. Royal Society of Chemistry 2016-04-11 Article PeerReviewed Patiño, Tania, Soriano, Jorge, Amirthalingam, Ezhil, Duran, Sara, González-Campo, Arántzazu, Duch, Marta, Ibáñez, Elena, Barrios, Leonardo, Plaza, Jose Antonio, Pérez-García, Lluïsa and Nogués, Carme (2016) Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications. Nanoscale, 8 (16). pp. 8773-8783. ISSN 2040-3372 http://pubs.rsc.org/en/Content/ArticleLanding/2016/NR/C5NR09022A#!divAbstract doi:10.1039/c5nr09022a doi:10.1039/c5nr09022a
spellingShingle Patiño, Tania
Soriano, Jorge
Amirthalingam, Ezhil
Duran, Sara
González-Campo, Arántzazu
Duch, Marta
Ibáñez, Elena
Barrios, Leonardo
Plaza, Jose Antonio
Pérez-García, Lluïsa
Nogués, Carme
Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications
title Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications
title_full Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications
title_fullStr Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications
title_full_unstemmed Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications
title_short Polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications
title_sort polysilicon-chromium-gold intracellular chips for multi-functional biomedical applications
url https://eprints.nottingham.ac.uk/52914/
https://eprints.nottingham.ac.uk/52914/
https://eprints.nottingham.ac.uk/52914/