Optical sectioning in multi-foci Raman hyperspectral imaging
In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was employed for the generation of multi-foci laser beams,...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
Wiley
2018
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/52774/ |
| _version_ | 1848798806596911104 |
|---|---|
| author | Liao, Zhiyu Sinjab, Faris Elsheikha, Hany M. Notingher, Ioan |
| author_facet | Liao, Zhiyu Sinjab, Faris Elsheikha, Hany M. Notingher, Ioan |
| author_sort | Liao, Zhiyu |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was employed for the generation of multi-foci laser beams, and a digital micromirror device (DMD) was used as a software-configurable reflective pinhole array. The patterns of the laser-foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance-to-size ratios were tested and compared. After optimisation of the laser foci pattern, we demonstrated the feasibility of multi-foci Raman hyperspectral microscopy for recording depth-resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth-discrimination and short acquisition times (20 minutes for single plane confocal image) was achieved. |
| first_indexed | 2025-11-14T20:25:38Z |
| format | Article |
| id | nottingham-52774 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:25:38Z |
| publishDate | 2018 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-527742020-05-04T19:46:42Z https://eprints.nottingham.ac.uk/52774/ Optical sectioning in multi-foci Raman hyperspectral imaging Liao, Zhiyu Sinjab, Faris Elsheikha, Hany M. Notingher, Ioan In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was employed for the generation of multi-foci laser beams, and a digital micromirror device (DMD) was used as a software-configurable reflective pinhole array. The patterns of the laser-foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance-to-size ratios were tested and compared. After optimisation of the laser foci pattern, we demonstrated the feasibility of multi-foci Raman hyperspectral microscopy for recording depth-resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth-discrimination and short acquisition times (20 minutes for single plane confocal image) was achieved. Wiley 2018-07-18 Article PeerReviewed Liao, Zhiyu, Sinjab, Faris, Elsheikha, Hany M. and Notingher, Ioan (2018) Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy . ISSN 1097-4555 Confocal Raman imaging; optical sectioning; multi-beam; cross-talk; single cell imaging https://onlinelibrary.wiley.com/doi/full/10.1002/jrs.5450 doi:10.1002/jrs.5450 doi:10.1002/jrs.5450 |
| spellingShingle | Confocal Raman imaging; optical sectioning; multi-beam; cross-talk; single cell imaging Liao, Zhiyu Sinjab, Faris Elsheikha, Hany M. Notingher, Ioan Optical sectioning in multi-foci Raman hyperspectral imaging |
| title | Optical sectioning in multi-foci Raman hyperspectral imaging |
| title_full | Optical sectioning in multi-foci Raman hyperspectral imaging |
| title_fullStr | Optical sectioning in multi-foci Raman hyperspectral imaging |
| title_full_unstemmed | Optical sectioning in multi-foci Raman hyperspectral imaging |
| title_short | Optical sectioning in multi-foci Raman hyperspectral imaging |
| title_sort | optical sectioning in multi-foci raman hyperspectral imaging |
| topic | Confocal Raman imaging; optical sectioning; multi-beam; cross-talk; single cell imaging |
| url | https://eprints.nottingham.ac.uk/52774/ https://eprints.nottingham.ac.uk/52774/ https://eprints.nottingham.ac.uk/52774/ |