DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase
Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subs...
| Main Authors: | , , , , |
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| Format: | Article |
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Taylor & Francis
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52737/ |
| _version_ | 1848798797063258112 |
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| author | Killelea, Tom Hawkins, Michelle Howard, Jamieson McGlynn, Peter Bolt, Edward L. |
| author_facet | Killelea, Tom Hawkins, Michelle Howard, Jamieson McGlynn, Peter Bolt, Edward L. |
| author_sort | Killelea, Tom |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity. |
| first_indexed | 2025-11-14T20:25:28Z |
| format | Article |
| id | nottingham-52737 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:25:28Z |
| publishDate | 2018 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-527372020-05-04T19:42:55Z https://eprints.nottingham.ac.uk/52737/ DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase Killelea, Tom Hawkins, Michelle Howard, Jamieson McGlynn, Peter Bolt, Edward L. Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity. Taylor & Francis 2018-08-10 Article PeerReviewed Killelea, Tom, Hawkins, Michelle, Howard, Jamieson, McGlynn, Peter and Bolt, Edward L. (2018) DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase. RNA Biology . ISSN 1555-8584 https://www.tandfonline.com/doi/full/10.1080/15476286.2018.1496773 doi:10.1080/15476286.2018.1496773 doi:10.1080/15476286.2018.1496773 |
| spellingShingle | Killelea, Tom Hawkins, Michelle Howard, Jamieson McGlynn, Peter Bolt, Edward L. DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase |
| title | DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase |
| title_full | DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase |
| title_fullStr | DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase |
| title_full_unstemmed | DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase |
| title_short | DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase |
| title_sort | dna replication roadblocks caused by cascade interference complexes are alleviated by recg dna repair helicase |
| url | https://eprints.nottingham.ac.uk/52737/ https://eprints.nottingham.ac.uk/52737/ https://eprints.nottingham.ac.uk/52737/ |