Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoofhorn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1_) and virulent...
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| Format: | Article |
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PeerJ
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52711/ |
| _version_ | 1848798791930478592 |
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| author | Agbaje, Michael Rutland, Catrin S. Maboni, Grazieli Blanchard, Adam Bexon, Melissa Stewart, Ceri Jones, Michael A. Totemeyer, Sabine |
| author_facet | Agbaje, Michael Rutland, Catrin S. Maboni, Grazieli Blanchard, Adam Bexon, Melissa Stewart, Ceri Jones, Michael A. Totemeyer, Sabine |
| author_sort | Agbaje, Michael |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoofhorn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1_) and virulent Dichelobacter nodosus in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1_ expression levels. Increased inflammatory score corresponded with high virulent D. nodosus load and was significant (p<0:0001) in footrot feet with an inflammatory score of 3 compared to scores 1 and 2. In addition, in contrast to healthy tissues, localisation of eubacterial load extended beyond follicular depths in footrot samples. The novel inflammatory cell infiltration scoring system in this study may be used to grade inflammatory response in the ovine feet and demonstrated an association between severity of inflammatory response and increased virulent D. nodosus load. |
| first_indexed | 2025-11-14T20:25:24Z |
| format | Article |
| id | nottingham-52711 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:25:24Z |
| publishDate | 2018 |
| publisher | PeerJ |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-527112024-08-15T15:30:30Z https://eprints.nottingham.ac.uk/52711/ Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin Agbaje, Michael Rutland, Catrin S. Maboni, Grazieli Blanchard, Adam Bexon, Melissa Stewart, Ceri Jones, Michael A. Totemeyer, Sabine Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoofhorn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1_) and virulent Dichelobacter nodosus in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1_ expression levels. Increased inflammatory score corresponded with high virulent D. nodosus load and was significant (p<0:0001) in footrot feet with an inflammatory score of 3 compared to scores 1 and 2. In addition, in contrast to healthy tissues, localisation of eubacterial load extended beyond follicular depths in footrot samples. The novel inflammatory cell infiltration scoring system in this study may be used to grade inflammatory response in the ovine feet and demonstrated an association between severity of inflammatory response and increased virulent D. nodosus load. PeerJ 2018-07-02 Article PeerReviewed Agbaje, Michael, Rutland, Catrin S., Maboni, Grazieli, Blanchard, Adam, Bexon, Melissa, Stewart, Ceri, Jones, Michael A. and Totemeyer, Sabine (2018) Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin. PeerJ, 6 . e5097. ISSN 2167-8359 Footrot Pro-inflammatory cytokine Dichelobacter nodosus Inflammation Histopathology Ovine https://peerj.com/articles/5097/ doi:10.7717/peerj.5097 doi:10.7717/peerj.5097 |
| spellingShingle | Footrot Pro-inflammatory cytokine Dichelobacter nodosus Inflammation Histopathology Ovine Agbaje, Michael Rutland, Catrin S. Maboni, Grazieli Blanchard, Adam Bexon, Melissa Stewart, Ceri Jones, Michael A. Totemeyer, Sabine Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin |
| title | Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin |
| title_full | Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin |
| title_fullStr | Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin |
| title_full_unstemmed | Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin |
| title_short | Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin |
| title_sort | novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin |
| topic | Footrot Pro-inflammatory cytokine Dichelobacter nodosus Inflammation Histopathology Ovine |
| url | https://eprints.nottingham.ac.uk/52711/ https://eprints.nottingham.ac.uk/52711/ https://eprints.nottingham.ac.uk/52711/ |