The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance
Although Yersinia enterocolitica genomes are highly heterogeneous, they contain a conserved N-acylhomoserine lactone-dependent (AHL) quorum sensing (QS) system consisting of the luxR and luxI orthologs yenR and yenI respectively. Certain hypervirulent strains also contain a putative orphan luxR gene...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
MDPI
2018
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/52493/ |
| _version_ | 1848798739380043776 |
|---|---|
| author | Ng, Yen-Kuan Grasso, Marco Wright, Victoria Garcia, Vanina Williams, Paul Atkinson, Steve |
| author_facet | Ng, Yen-Kuan Grasso, Marco Wright, Victoria Garcia, Vanina Williams, Paul Atkinson, Steve |
| author_sort | Ng, Yen-Kuan |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Although Yersinia enterocolitica genomes are highly heterogeneous, they contain a conserved N-acylhomoserine lactone-dependent (AHL) quorum sensing (QS) system consisting of the luxR and luxI orthologs yenR and yenI respectively. Certain hypervirulent strains also contain a putative orphan luxR gene, ycoR, that is not linked to an AHL synthase. To explore the contribution of yenR/yenI/ycoR to QS-dependent phenotypes in Yersinia enterocolitica strain 8081, single and multiple mutants were constructed. AHL profiling identified N-(3-oxohexanoyl) homoserine lactone, N-hexanoylhomoserine lactone, and N-(3-oxoseptanoyl) homoserine lactone as the most abundant. The AHL profiles of the yenR, ycoR and yenR/ycoR mutants were similar to the parent suggesting that the two LuxR homologues do not regulate AHL production while the yenI mutants were AHL-negative. A role for QS in swimming motility and cell attachment was demonstrated. Down-regulation of the virulence plasmid partition gene, spyA, in yenI and yenI/yenR/ycoR mutants is consistent with the greater loss of the Y. enterocolitica pYVe virulence plasmid in the yenI mutant during serial passage at 37 C but not at 22 C. A role for QS-regulated spyA in virulence plasmid maintenance is suggested. |
| first_indexed | 2025-11-14T20:24:33Z |
| format | Article |
| id | nottingham-52493 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:24:33Z |
| publishDate | 2018 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-524932020-05-04T19:41:57Z https://eprints.nottingham.ac.uk/52493/ The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance Ng, Yen-Kuan Grasso, Marco Wright, Victoria Garcia, Vanina Williams, Paul Atkinson, Steve Although Yersinia enterocolitica genomes are highly heterogeneous, they contain a conserved N-acylhomoserine lactone-dependent (AHL) quorum sensing (QS) system consisting of the luxR and luxI orthologs yenR and yenI respectively. Certain hypervirulent strains also contain a putative orphan luxR gene, ycoR, that is not linked to an AHL synthase. To explore the contribution of yenR/yenI/ycoR to QS-dependent phenotypes in Yersinia enterocolitica strain 8081, single and multiple mutants were constructed. AHL profiling identified N-(3-oxohexanoyl) homoserine lactone, N-hexanoylhomoserine lactone, and N-(3-oxoseptanoyl) homoserine lactone as the most abundant. The AHL profiles of the yenR, ycoR and yenR/ycoR mutants were similar to the parent suggesting that the two LuxR homologues do not regulate AHL production while the yenI mutants were AHL-negative. A role for QS in swimming motility and cell attachment was demonstrated. Down-regulation of the virulence plasmid partition gene, spyA, in yenI and yenI/yenR/ycoR mutants is consistent with the greater loss of the Y. enterocolitica pYVe virulence plasmid in the yenI mutant during serial passage at 37 C but not at 22 C. A role for QS-regulated spyA in virulence plasmid maintenance is suggested. MDPI 2018-06-20 Article PeerReviewed Ng, Yen-Kuan, Grasso, Marco, Wright, Victoria, Garcia, Vanina, Williams, Paul and Atkinson, Steve (2018) The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance. Genes, 9 (6). 307/1-307/14. ISSN 2073-4425 Yersinia enterocolitica; Quorum sensing; LuxR; N-acylhomoserine lactones; Motility; Cell attachment; Virulence plasmid maintenance http://www.mdpi.com/2073-4425/9/6/307 doi:10.3390/genes9060307 doi:10.3390/genes9060307 |
| spellingShingle | Yersinia enterocolitica; Quorum sensing; LuxR; N-acylhomoserine lactones; Motility; Cell attachment; Virulence plasmid maintenance Ng, Yen-Kuan Grasso, Marco Wright, Victoria Garcia, Vanina Williams, Paul Atkinson, Steve The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance |
| title | The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance |
| title_full | The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance |
| title_fullStr | The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance |
| title_full_unstemmed | The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance |
| title_short | The quorum sensing system of Yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance |
| title_sort | quorum sensing system of yersinia enterocolitica 8081 regulates swimming motility, host cell attachment, and virulence plasmid maintenance |
| topic | Yersinia enterocolitica; Quorum sensing; LuxR; N-acylhomoserine lactones; Motility; Cell attachment; Virulence plasmid maintenance |
| url | https://eprints.nottingham.ac.uk/52493/ https://eprints.nottingham.ac.uk/52493/ https://eprints.nottingham.ac.uk/52493/ |