| Summary: | Current technology enables researchers and clinicians to be able to identify the presence of AD definitively in only post-mortem brain samples. Limitations occur when several biomarkers need to be identified simultaneously. The aims of this study were to develop a system in which several AD biomarkers can be targeted at the same time, on a number of different sample types. As a method, reverse phase protein micro-arrays (RPPAs) have been used extensively with cultured cell samples, however there is a deficiency with regards to using RPPA with brain tissue. Hence the specific aim of this study was to use RPPA in developing the detection method of a panel of AD biomarkers using brain samples from AD pathology mice.
The objectives of this project were to optimise the protein extraction methods, to concentrate the required proteins from other components found in brain samples. Validation of the biomarkers was to be done by western blotting techniques, prior to use in RPPA. The final objective was to then validate this method on tau pathology mice of hTau background.
By carrying out these experiments, I can hypothesise that the micro-arraying method will give high throughput results and allow important proteins found in the AD brain to be seen simultaneously.
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