Rapid one-step biotinylation of biological and non-biological surfaces
We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membran...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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Nature Publishing Group
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52262/ |
| _version_ | 1848798685863870464 |
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| author | Henry, Stephen Williams, Eleanor Barr, Katie Korchagina, Elena Tuzikov, Alexandr Ilyushina, Natalia Abayzeed, Sidahmed A. Webb, Kevin F. Bovin, Nicolai |
| author_facet | Henry, Stephen Williams, Eleanor Barr, Katie Korchagina, Elena Tuzikov, Alexandr Ilyushina, Natalia Abayzeed, Sidahmed A. Webb, Kevin F. Bovin, Nicolai |
| author_sort | Henry, Stephen |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells. |
| first_indexed | 2025-11-14T20:23:42Z |
| format | Article |
| id | nottingham-52262 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:23:42Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-522622020-05-04T19:31:51Z https://eprints.nottingham.ac.uk/52262/ Rapid one-step biotinylation of biological and non-biological surfaces Henry, Stephen Williams, Eleanor Barr, Katie Korchagina, Elena Tuzikov, Alexandr Ilyushina, Natalia Abayzeed, Sidahmed A. Webb, Kevin F. Bovin, Nicolai We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells. Nature Publishing Group 2018-02-12 Article PeerReviewed Henry, Stephen, Williams, Eleanor, Barr, Katie, Korchagina, Elena, Tuzikov, Alexandr, Ilyushina, Natalia, Abayzeed, Sidahmed A., Webb, Kevin F. and Bovin, Nicolai (2018) Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8 (1). 2845/1-2845/6. ISSN 2045-2322 https://doi.org/10.1038/s41598-018-21186-3 doi:10.1038/s41598-018-21186-3 doi:10.1038/s41598-018-21186-3 |
| spellingShingle | Henry, Stephen Williams, Eleanor Barr, Katie Korchagina, Elena Tuzikov, Alexandr Ilyushina, Natalia Abayzeed, Sidahmed A. Webb, Kevin F. Bovin, Nicolai Rapid one-step biotinylation of biological and non-biological surfaces |
| title | Rapid one-step biotinylation of biological and non-biological surfaces |
| title_full | Rapid one-step biotinylation of biological and non-biological surfaces |
| title_fullStr | Rapid one-step biotinylation of biological and non-biological surfaces |
| title_full_unstemmed | Rapid one-step biotinylation of biological and non-biological surfaces |
| title_short | Rapid one-step biotinylation of biological and non-biological surfaces |
| title_sort | rapid one-step biotinylation of biological and non-biological surfaces |
| url | https://eprints.nottingham.ac.uk/52262/ https://eprints.nottingham.ac.uk/52262/ https://eprints.nottingham.ac.uk/52262/ |