High-throughput miniaturized screening of nanoparticle formation via inkjet printing
The self‐assembly of specific polymers into well‐defined nanoparticles (NPs) is of great interest to the pharmaceutical industry as the resultant materials can act as drug delivery vehicles. In this work, a high‐throughput method to screen the ability of polymers to self‐assemble into NPs using a pi...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
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Wiley-VCH
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52245/ |
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| author | Styliari, Ioanna D. Conte, Claudia Pearce, Amanda K. Hüsler, Amanda Cavanagh, Robert J. Limo, Marion J. Gordhan, Dipak Nieto-Orellana, Alejandro Suksiriworapong, Jiraphong Couturaud, Benoit Williams, Phil Hook, Andrew L. Alexander, Morgan R. Garnett, Martin C. Alexander, Cameron Burley, Jonathan C. Taresco, Vincenzo |
| author_facet | Styliari, Ioanna D. Conte, Claudia Pearce, Amanda K. Hüsler, Amanda Cavanagh, Robert J. Limo, Marion J. Gordhan, Dipak Nieto-Orellana, Alejandro Suksiriworapong, Jiraphong Couturaud, Benoit Williams, Phil Hook, Andrew L. Alexander, Morgan R. Garnett, Martin C. Alexander, Cameron Burley, Jonathan C. Taresco, Vincenzo |
| author_sort | Styliari, Ioanna D. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The self‐assembly of specific polymers into well‐defined nanoparticles (NPs) is of great interest to the pharmaceutical industry as the resultant materials can act as drug delivery vehicles. In this work, a high‐throughput method to screen the ability of polymers to self‐assemble into NPs using a picoliter inkjet printer is presented. By dispensing polymer solutions in dimethyl sulfoxide (DMSO) from the printer into the wells of a 96‐well plate, containing water as an antisolvent, 50 suspensions are screened for nanoparticle formation rapidly using only nanoliters to microliters. A variety of polymer classes are used and in situ characterization of the submicroliter nanosuspensions shows that the particle size distributions match those of nanoparticles made from bulk suspensions. Dispensing organic polymer solutions into well plates via the printer is thus shown to be a reproducible and fast method for screening nanoparticle formation which uses two to three orders of magnitude less material than conventional techniques. Finally, a pilot study for a high‐throughput pipeline of nanoparticle production, physical property characterization, and cytocompatibility demonstrates the feasibility of the printing approach for screening of nanodrug delivery formulations. Nanoparticles are produced in the well plates, characterized for size and evaluated for effects on metabolic activity of lung cancer cells. |
| first_indexed | 2025-11-14T20:23:39Z |
| format | Article |
| id | nottingham-52245 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:23:39Z |
| publishDate | 2018 |
| publisher | Wiley-VCH |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-522452020-05-04T19:37:53Z https://eprints.nottingham.ac.uk/52245/ High-throughput miniaturized screening of nanoparticle formation via inkjet printing Styliari, Ioanna D. Conte, Claudia Pearce, Amanda K. Hüsler, Amanda Cavanagh, Robert J. Limo, Marion J. Gordhan, Dipak Nieto-Orellana, Alejandro Suksiriworapong, Jiraphong Couturaud, Benoit Williams, Phil Hook, Andrew L. Alexander, Morgan R. Garnett, Martin C. Alexander, Cameron Burley, Jonathan C. Taresco, Vincenzo The self‐assembly of specific polymers into well‐defined nanoparticles (NPs) is of great interest to the pharmaceutical industry as the resultant materials can act as drug delivery vehicles. In this work, a high‐throughput method to screen the ability of polymers to self‐assemble into NPs using a picoliter inkjet printer is presented. By dispensing polymer solutions in dimethyl sulfoxide (DMSO) from the printer into the wells of a 96‐well plate, containing water as an antisolvent, 50 suspensions are screened for nanoparticle formation rapidly using only nanoliters to microliters. A variety of polymer classes are used and in situ characterization of the submicroliter nanosuspensions shows that the particle size distributions match those of nanoparticles made from bulk suspensions. Dispensing organic polymer solutions into well plates via the printer is thus shown to be a reproducible and fast method for screening nanoparticle formation which uses two to three orders of magnitude less material than conventional techniques. Finally, a pilot study for a high‐throughput pipeline of nanoparticle production, physical property characterization, and cytocompatibility demonstrates the feasibility of the printing approach for screening of nanodrug delivery formulations. Nanoparticles are produced in the well plates, characterized for size and evaluated for effects on metabolic activity of lung cancer cells. Wiley-VCH 2018-08 Article PeerReviewed Styliari, Ioanna D., Conte, Claudia, Pearce, Amanda K., Hüsler, Amanda, Cavanagh, Robert J., Limo, Marion J., Gordhan, Dipak, Nieto-Orellana, Alejandro, Suksiriworapong, Jiraphong, Couturaud, Benoit, Williams, Phil, Hook, Andrew L., Alexander, Morgan R., Garnett, Martin C., Alexander, Cameron, Burley, Jonathan C. and Taresco, Vincenzo (2018) High-throughput miniaturized screening of nanoparticle formation via inkjet printing. Macromolecular Materials and Engineering, 303 (8). ISSN 1439-2054 High-throughput miniaturized screening; Inkjet printers; Nanoparticles; Self-assembling https://onlinelibrary.wiley.com/doi/full/10.1002/mame.201800146 doi:10.1002/mame.201800146 doi:10.1002/mame.201800146 |
| spellingShingle | High-throughput miniaturized screening; Inkjet printers; Nanoparticles; Self-assembling Styliari, Ioanna D. Conte, Claudia Pearce, Amanda K. Hüsler, Amanda Cavanagh, Robert J. Limo, Marion J. Gordhan, Dipak Nieto-Orellana, Alejandro Suksiriworapong, Jiraphong Couturaud, Benoit Williams, Phil Hook, Andrew L. Alexander, Morgan R. Garnett, Martin C. Alexander, Cameron Burley, Jonathan C. Taresco, Vincenzo High-throughput miniaturized screening of nanoparticle formation via inkjet printing |
| title | High-throughput miniaturized screening of nanoparticle formation via inkjet printing |
| title_full | High-throughput miniaturized screening of nanoparticle formation via inkjet printing |
| title_fullStr | High-throughput miniaturized screening of nanoparticle formation via inkjet printing |
| title_full_unstemmed | High-throughput miniaturized screening of nanoparticle formation via inkjet printing |
| title_short | High-throughput miniaturized screening of nanoparticle formation via inkjet printing |
| title_sort | high-throughput miniaturized screening of nanoparticle formation via inkjet printing |
| topic | High-throughput miniaturized screening; Inkjet printers; Nanoparticles; Self-assembling |
| url | https://eprints.nottingham.ac.uk/52245/ https://eprints.nottingham.ac.uk/52245/ https://eprints.nottingham.ac.uk/52245/ |