Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing
Here we describe a rapid inkjet formulation method for screening newly-synthesised cationic materials for siRNA delivery into cancer cells. Reduction responsive oligo-β-aminoesters were prepared and evaluated for their ability to condense siRNA into polyplexes through a fast inkjet printing method....
| Main Authors: | , , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52240/ |
| _version_ | 1848798680671322112 |
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| author | Lovato, Tatiana Taresco, Vincenzo Alazzo, Ali Sansone, Catarina Stolnik, Snjezana Alexander, Cameron Conte, Claudia |
| author_facet | Lovato, Tatiana Taresco, Vincenzo Alazzo, Ali Sansone, Catarina Stolnik, Snjezana Alexander, Cameron Conte, Claudia |
| author_sort | Lovato, Tatiana |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Here we describe a rapid inkjet formulation method for screening newly-synthesised cationic materials for siRNA delivery into cancer cells. Reduction responsive oligo-β-aminoesters were prepared and evaluated for their ability to condense siRNA into polyplexes through a fast inkjet printing method. A direct relationship between the oligomer structures and charge densities, and the final cell response in terms of uptake rate and transfection efficacy, was found. The oligo-β-aminoesters were well-tolerated by the cancer cells, compared to conventional cationic polymers so far employed in gene delivery, and were as active in silencing of a representative luciferase gene. |
| first_indexed | 2025-11-14T20:23:37Z |
| format | Article |
| id | nottingham-52240 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:23:37Z |
| publishDate | 2018 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-522402020-05-04T19:38:47Z https://eprints.nottingham.ac.uk/52240/ Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing Lovato, Tatiana Taresco, Vincenzo Alazzo, Ali Sansone, Catarina Stolnik, Snjezana Alexander, Cameron Conte, Claudia Here we describe a rapid inkjet formulation method for screening newly-synthesised cationic materials for siRNA delivery into cancer cells. Reduction responsive oligo-β-aminoesters were prepared and evaluated for their ability to condense siRNA into polyplexes through a fast inkjet printing method. A direct relationship between the oligomer structures and charge densities, and the final cell response in terms of uptake rate and transfection efficacy, was found. The oligo-β-aminoesters were well-tolerated by the cancer cells, compared to conventional cationic polymers so far employed in gene delivery, and were as active in silencing of a representative luciferase gene. Royal Society of Chemistry 2018-06-01 Article PeerReviewed Lovato, Tatiana, Taresco, Vincenzo, Alazzo, Ali, Sansone, Catarina, Stolnik, Snjezana, Alexander, Cameron and Conte, Claudia (2018) Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing. Journal of Materials Chemistry B . ISSN 2050-7518 (In Press) |
| spellingShingle | Lovato, Tatiana Taresco, Vincenzo Alazzo, Ali Sansone, Catarina Stolnik, Snjezana Alexander, Cameron Conte, Claudia Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing |
| title | Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing |
| title_full | Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing |
| title_fullStr | Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing |
| title_full_unstemmed | Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing |
| title_short | Rapid formulation of redox-responsive oligo-β-aminoester polyplexes with siRNA via jet printing |
| title_sort | rapid formulation of redox-responsive oligo-β-aminoester polyplexes with sirna via jet printing |
| url | https://eprints.nottingham.ac.uk/52240/ |