Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network
The Arabidopsis homeodomain transcription factor SHOOT MERISTEMLESS (STM) is crucial for shoot apical meristem (SAM) function, yet the components and structure of the STMgene regulatory network (GRN) are largely unknown. Here, we show that transcriptional regulators are overrepresented among STM-reg...
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| Format: | Article |
| Language: | English |
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Company of Biologists
2018
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| Online Access: | https://eprints.nottingham.ac.uk/52102/ |
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| author | Scofield, Simon Murison, Alexander Jones, Angharad Fozard, John Aida, Mitsuhiro Band, Leah R. Bennett, Malcolm Murray, James A.H. |
| author_facet | Scofield, Simon Murison, Alexander Jones, Angharad Fozard, John Aida, Mitsuhiro Band, Leah R. Bennett, Malcolm Murray, James A.H. |
| author_sort | Scofield, Simon |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The Arabidopsis homeodomain transcription factor SHOOT MERISTEMLESS (STM) is crucial for shoot apical meristem (SAM) function, yet the components and structure of the STMgene regulatory network (GRN) are largely unknown. Here, we show that transcriptional regulators are overrepresented among STM-regulated genes and, using these as GRN components in Bayesian network analysis, we infer STM GRN associations and reveal regulatory relationships between STM and factors involved in multiple aspects of SAM function. These include hormone regulation, TCP-mediated control of cell differentiation, AIL/PLT-mediated regulation of pluripotency and phyllotaxis, and specification of meristem-organ boundary zones via CUC1. We demonstrate a direct positive transcriptional feedback loop between STM and CUC1, despite their distinct expression patterns in the meristem and organ boundary, respectively. Our further finding that STM activates expression of the CUC1-targeting microRNA miR164c combined with mathematical modelling provides a potential solution for this apparent contradiction, demonstrating that these proposed regulatory interactions coupled with STM mobility could be sufficient to provide a mechanism for CUC1 localisation at the meristem-organ boundary. Our findings highlight the central role for the STM GRN in coordinating SAM functions. |
| first_indexed | 2025-11-14T20:23:07Z |
| format | Article |
| id | nottingham-52102 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:23:07Z |
| publishDate | 2018 |
| publisher | Company of Biologists |
| recordtype | eprints |
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| spelling | nottingham-521022018-05-31T17:21:17Z https://eprints.nottingham.ac.uk/52102/ Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network Scofield, Simon Murison, Alexander Jones, Angharad Fozard, John Aida, Mitsuhiro Band, Leah R. Bennett, Malcolm Murray, James A.H. The Arabidopsis homeodomain transcription factor SHOOT MERISTEMLESS (STM) is crucial for shoot apical meristem (SAM) function, yet the components and structure of the STMgene regulatory network (GRN) are largely unknown. Here, we show that transcriptional regulators are overrepresented among STM-regulated genes and, using these as GRN components in Bayesian network analysis, we infer STM GRN associations and reveal regulatory relationships between STM and factors involved in multiple aspects of SAM function. These include hormone regulation, TCP-mediated control of cell differentiation, AIL/PLT-mediated regulation of pluripotency and phyllotaxis, and specification of meristem-organ boundary zones via CUC1. We demonstrate a direct positive transcriptional feedback loop between STM and CUC1, despite their distinct expression patterns in the meristem and organ boundary, respectively. Our further finding that STM activates expression of the CUC1-targeting microRNA miR164c combined with mathematical modelling provides a potential solution for this apparent contradiction, demonstrating that these proposed regulatory interactions coupled with STM mobility could be sufficient to provide a mechanism for CUC1 localisation at the meristem-organ boundary. Our findings highlight the central role for the STM GRN in coordinating SAM functions. Company of Biologists 2018-04-30 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/52102/1/dev157081.full.pdf Scofield, Simon, Murison, Alexander, Jones, Angharad, Fozard, John, Aida, Mitsuhiro, Band, Leah R., Bennett, Malcolm and Murray, James A.H. (2018) Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network. Development, 145 (9). dev157081. ISSN 0950-1991 SHOOT MERISTEMLESS Gene regulatory network Arabidopsis Homeodomain Meristem KNOX Transcriptional regulation http://dev.biologists.org/content/145/9/dev157081 doi:10.1242/dev.157081 doi:10.1242/dev.157081 |
| spellingShingle | SHOOT MERISTEMLESS Gene regulatory network Arabidopsis Homeodomain Meristem KNOX Transcriptional regulation Scofield, Simon Murison, Alexander Jones, Angharad Fozard, John Aida, Mitsuhiro Band, Leah R. Bennett, Malcolm Murray, James A.H. Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
| title | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
| title_full | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
| title_fullStr | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
| title_full_unstemmed | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
| title_short | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
| title_sort | coordination of meristem and boundary functions by transcription factors in the shoot meristemless regulatory network |
| topic | SHOOT MERISTEMLESS Gene regulatory network Arabidopsis Homeodomain Meristem KNOX Transcriptional regulation |
| url | https://eprints.nottingham.ac.uk/52102/ https://eprints.nottingham.ac.uk/52102/ https://eprints.nottingham.ac.uk/52102/ |