Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis
Background: Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent mo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Hindawi
2017
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| Online Access: | https://eprints.nottingham.ac.uk/51956/ |
| _version_ | 1848798612846280704 |
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| author | Chapman, Victoria Markides, Hareklea Sagar, Devi Rani Xu, Luting Burston, James J. Mapp, Paul Kay, Alasdair Morris, Robert H. Kehoe, Oksana El Haj, Alicia J. |
| author_facet | Chapman, Victoria Markides, Hareklea Sagar, Devi Rani Xu, Luting Burston, James J. Mapp, Paul Kay, Alasdair Morris, Robert H. Kehoe, Oksana El Haj, Alicia J. |
| author_sort | Chapman, Victoria |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background: Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent model of OA.
Methods: Rats underwent either medial meniscal transection (MNX) or sham surgery under anaesthesia. Rats received intra-articular injection of either 1.5×106 late passage MSCs labelled with 10 μg/ml SiMAG, 1.5×106 late passage mesenchymal stem cells, the steroid Kenalog (200 μg/20 μL), 1.5×106 early passage MSCs, or serum-free media (SFM). Sham-operated rats received intra-articular injection of SFM. Pain behaviour was quantified until day 42 postmodel induction. Magnetic resonance imaging (MRI) was used to localise the labelled cells within the knee joint.
Results: Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period. Early passage MSCs exacerbated MNX-induced pain behaviour for up to one week postinjection and did not alter joint pathology.
Conclusion: Our data demonstrate for the first time the role of a passage number in influencing the therapeutic effects of MSCs in a model of OA pain. |
| first_indexed | 2025-11-14T20:22:33Z |
| format | Article |
| id | nottingham-51956 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:22:33Z |
| publishDate | 2017 |
| publisher | Hindawi |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-519562020-05-04T19:23:55Z https://eprints.nottingham.ac.uk/51956/ Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis Chapman, Victoria Markides, Hareklea Sagar, Devi Rani Xu, Luting Burston, James J. Mapp, Paul Kay, Alasdair Morris, Robert H. Kehoe, Oksana El Haj, Alicia J. Background: Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent model of OA. Methods: Rats underwent either medial meniscal transection (MNX) or sham surgery under anaesthesia. Rats received intra-articular injection of either 1.5×106 late passage MSCs labelled with 10 μg/ml SiMAG, 1.5×106 late passage mesenchymal stem cells, the steroid Kenalog (200 μg/20 μL), 1.5×106 early passage MSCs, or serum-free media (SFM). Sham-operated rats received intra-articular injection of SFM. Pain behaviour was quantified until day 42 postmodel induction. Magnetic resonance imaging (MRI) was used to localise the labelled cells within the knee joint. Results: Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period. Early passage MSCs exacerbated MNX-induced pain behaviour for up to one week postinjection and did not alter joint pathology. Conclusion: Our data demonstrate for the first time the role of a passage number in influencing the therapeutic effects of MSCs in a model of OA pain. Hindawi 2017-12-24 Article PeerReviewed Chapman, Victoria, Markides, Hareklea, Sagar, Devi Rani, Xu, Luting, Burston, James J., Mapp, Paul, Kay, Alasdair, Morris, Robert H., Kehoe, Oksana and El Haj, Alicia J. (2017) Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis. Stem Cells International, 2017 . p. 2905104. ISSN 1687-966X https://www.hindawi.com/journals/sci/2017/2905104/ doi:10.1155/2017/2905104 doi:10.1155/2017/2905104 |
| spellingShingle | Chapman, Victoria Markides, Hareklea Sagar, Devi Rani Xu, Luting Burston, James J. Mapp, Paul Kay, Alasdair Morris, Robert H. Kehoe, Oksana El Haj, Alicia J. Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis |
| title | Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis |
| title_full | Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis |
| title_fullStr | Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis |
| title_full_unstemmed | Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis |
| title_short | Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis |
| title_sort | therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis |
| url | https://eprints.nottingham.ac.uk/51956/ https://eprints.nottingham.ac.uk/51956/ https://eprints.nottingham.ac.uk/51956/ |