3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy
Many different nanoparticle delivery systems have been reported as potential cancer therapeutics, however, the tumour penetration and uptake characteristics have been determined for very few systems. Animal models are effective for assessing tumour localisation of nanosystems, but difficult to use f...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2018
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| Online Access: | https://eprints.nottingham.ac.uk/51750/ |
| _version_ | 1848798565582766080 |
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| author | Tchoryk, Aleksandra |
| author_facet | Tchoryk, Aleksandra |
| author_sort | Tchoryk, Aleksandra |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Many different nanoparticle delivery systems have been reported as potential cancer therapeutics, however, the tumour penetration and uptake characteristics have been determined for very few systems. Animal models are effective for assessing tumour localisation of nanosystems, but difficult to use for studying penetration beyond the vasculature. In this work, defined HCT 116 colorectal cancer spheroids were used to study the effect of nanoparticle size and surface modifications on their penetration and uptake. Incubation of spheroids with Hoechst 33342 resulted in a dye gradient which facilitated discrimination between the populations of cells in the core and at the periphery of spheroids by flow cytometry based on the degree of Hoechst staining. This model was used to compare doxorubicin and Doxil, a range of model polystyrene nanoparticles in different sizes (30 nm, 50 nm, 100 nm) and with different surface chemistry (50 nm unmodified, carboxylated, aminated) and polyethylene glycol modified NPs prepared from a promising new functionalized biodegradable polymer (poly(glycerol-adipate), PGA). Unmodified polystyrene nanoparticles (30 nm/50 nm) were able to penetrate to the core of HCT 116 spheroids more efficiently than larger polystyrene nanoparticles (100 nm). Penetration was also dependent on surface charge. PGA NPs of 100 nm showed similar penetration into spheroids as 50 nm polystyrene nanoparticles, and PEG surface modification significantly improved penetration into the spheroid core. The new spheroid model with Hoechst staining is shown to be a useful model for assessing NPs penetration and demonstrates the importance of controlling physical properties when designing nanomedicine. |
| first_indexed | 2025-11-14T20:21:48Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-51750 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:21:48Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-517502025-02-28T14:06:52Z https://eprints.nottingham.ac.uk/51750/ 3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy Tchoryk, Aleksandra Many different nanoparticle delivery systems have been reported as potential cancer therapeutics, however, the tumour penetration and uptake characteristics have been determined for very few systems. Animal models are effective for assessing tumour localisation of nanosystems, but difficult to use for studying penetration beyond the vasculature. In this work, defined HCT 116 colorectal cancer spheroids were used to study the effect of nanoparticle size and surface modifications on their penetration and uptake. Incubation of spheroids with Hoechst 33342 resulted in a dye gradient which facilitated discrimination between the populations of cells in the core and at the periphery of spheroids by flow cytometry based on the degree of Hoechst staining. This model was used to compare doxorubicin and Doxil, a range of model polystyrene nanoparticles in different sizes (30 nm, 50 nm, 100 nm) and with different surface chemistry (50 nm unmodified, carboxylated, aminated) and polyethylene glycol modified NPs prepared from a promising new functionalized biodegradable polymer (poly(glycerol-adipate), PGA). Unmodified polystyrene nanoparticles (30 nm/50 nm) were able to penetrate to the core of HCT 116 spheroids more efficiently than larger polystyrene nanoparticles (100 nm). Penetration was also dependent on surface charge. PGA NPs of 100 nm showed similar penetration into spheroids as 50 nm polystyrene nanoparticles, and PEG surface modification significantly improved penetration into the spheroid core. The new spheroid model with Hoechst staining is shown to be a useful model for assessing NPs penetration and demonstrates the importance of controlling physical properties when designing nanomedicine. 2018-07-20 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/51750/1/Aleksandra%20Tchoryk-%204212449.pdf Tchoryk, Aleksandra (2018) 3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy. PhD thesis, University of Nottingham. 3D spheroids nanoparticles delivery tumour penetration cell uptake drug delivery |
| spellingShingle | 3D spheroids nanoparticles delivery tumour penetration cell uptake drug delivery Tchoryk, Aleksandra 3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy |
| title | 3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy |
| title_full | 3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy |
| title_fullStr | 3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy |
| title_full_unstemmed | 3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy |
| title_short | 3D spheroid models for in vitro evaluation of nanoparticles for cancer therapy |
| title_sort | 3d spheroid models for in vitro evaluation of nanoparticles for cancer therapy |
| topic | 3D spheroids nanoparticles delivery tumour penetration cell uptake drug delivery |
| url | https://eprints.nottingham.ac.uk/51750/ |