Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity
Objective Spondias mombin L. is a tree used in folk medicine in Nigeria for the treatment of hepatitis. This study was carried out to comparatively evaluate the hepatoprotective and antioxidant effects of S. mombin leaf and stem (SML and SMS) methanolic extracts in a rat model of carbon tetrachlo...
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| Format: | Article |
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Elsevier
2018
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| Online Access: | https://eprints.nottingham.ac.uk/51617/ |
| _version_ | 1848798533715492864 |
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| author | Nwidu, Lucky Legbosi Elmorsy, Ekramy Yibala, Oboma I. Carter, Wayne |
| author_facet | Nwidu, Lucky Legbosi Elmorsy, Ekramy Yibala, Oboma I. Carter, Wayne |
| author_sort | Nwidu, Lucky Legbosi |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Objective
Spondias mombin L. is a tree used in folk medicine in Nigeria for the treatment of hepatitis. This study was carried out to comparatively evaluate the hepatoprotective and antioxidant effects of S. mombin leaf and stem (SML and SMS) methanolic extracts in a rat model of carbon tetrachloride (CCl4)-induced hepatotoxicity.
Methods
Forty-two rats were distributed into seven groups. Groups A and B received water; groups C and D received 500 and 1000 mg/kg SML extract, respectively; groups E and F received 500 and 1000 mg/kg SMS extract, respectively; and group G received 100 mg/kg silymarin. Water, the two extracts, and silymarin were administered daily by oral gavage for 7 days. Hepatotoxicity was induced in groups B to G by the administration of CCl4 once on the seventh day. After 48 h, rats were sacrificed, and tissues and serum samples were examined for histological and biochemical indices of hepatotoxicity.
Results
Administration of CCl4 resulted in liver injury with significant elevation in the hepatocellular injury markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TBIL), and conjugated bilirubin (CBIL), associated with a significant reduction in total circulatory protein. Pretreatment with SML and SMS extracts at both doses significantly ameliorated liver injury; lowered ALT, AST, ALP, TBIL, and CBIL levels; elevated cellular glutathione levels as well as catalase and superoxide dismutase activities; and decreased the levels of thiobarbituric acid reactive substances.
Conclusion
This study provides preliminary evidence supporting the potential therapeutic benefit of S. mombin in xenobiotic-induced hepatotoxicity. |
| first_indexed | 2025-11-14T20:21:17Z |
| format | Article |
| id | nottingham-51617 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:21:17Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-516172020-05-04T19:50:59Z https://eprints.nottingham.ac.uk/51617/ Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity Nwidu, Lucky Legbosi Elmorsy, Ekramy Yibala, Oboma I. Carter, Wayne Objective Spondias mombin L. is a tree used in folk medicine in Nigeria for the treatment of hepatitis. This study was carried out to comparatively evaluate the hepatoprotective and antioxidant effects of S. mombin leaf and stem (SML and SMS) methanolic extracts in a rat model of carbon tetrachloride (CCl4)-induced hepatotoxicity. Methods Forty-two rats were distributed into seven groups. Groups A and B received water; groups C and D received 500 and 1000 mg/kg SML extract, respectively; groups E and F received 500 and 1000 mg/kg SMS extract, respectively; and group G received 100 mg/kg silymarin. Water, the two extracts, and silymarin were administered daily by oral gavage for 7 days. Hepatotoxicity was induced in groups B to G by the administration of CCl4 once on the seventh day. After 48 h, rats were sacrificed, and tissues and serum samples were examined for histological and biochemical indices of hepatotoxicity. Results Administration of CCl4 resulted in liver injury with significant elevation in the hepatocellular injury markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TBIL), and conjugated bilirubin (CBIL), associated with a significant reduction in total circulatory protein. Pretreatment with SML and SMS extracts at both doses significantly ameliorated liver injury; lowered ALT, AST, ALP, TBIL, and CBIL levels; elevated cellular glutathione levels as well as catalase and superoxide dismutase activities; and decreased the levels of thiobarbituric acid reactive substances. Conclusion This study provides preliminary evidence supporting the potential therapeutic benefit of S. mombin in xenobiotic-induced hepatotoxicity. Elsevier 2018-06 Article PeerReviewed Nwidu, Lucky Legbosi, Elmorsy, Ekramy, Yibala, Oboma I. and Carter, Wayne (2018) Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity. Journal of Taibah University Medical Sciences, 13 (3). pp. 262-271. ISSN 1658-3612 Hepatoptection; hepatotoxicity; oxidative stress; Spondias mombin L. https://www.sciencedirect.com/science/article/pii/S1658361218300313?via%3Dihub doi:10.1016/j.jtumed.2018.03.006 doi:10.1016/j.jtumed.2018.03.006 |
| spellingShingle | Hepatoptection; hepatotoxicity; oxidative stress; Spondias mombin L. Nwidu, Lucky Legbosi Elmorsy, Ekramy Yibala, Oboma I. Carter, Wayne Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity |
| title | Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity |
| title_full | Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity |
| title_fullStr | Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity |
| title_full_unstemmed | Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity |
| title_short | Hepatoprotective and antioxidant activities of Spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity |
| title_sort | hepatoprotective and antioxidant activities of spondias mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity |
| topic | Hepatoptection; hepatotoxicity; oxidative stress; Spondias mombin L. |
| url | https://eprints.nottingham.ac.uk/51617/ https://eprints.nottingham.ac.uk/51617/ https://eprints.nottingham.ac.uk/51617/ |