VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis
Chronic pain can develop in response to conditions such as inflammatory arthritis. The central mechanisms underlying the development and maintenance of chronic pain in humans are not well elucidated although there is evidence for a role of microglia and astrocytes. However in pre-clinical models of...
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | https://eprints.nottingham.ac.uk/51439/ |
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| author | Beazley-Long, Nicholas Moss, Catherine Elizabeth Ashby, William Robert Bestall, Samuel Marcus Almahasneh, Fatimah Durrant, Alexandra Margaret Benest, Andrew Vaughan Blackley, Zoe Ballmer-Hofer, Kurt Hirashima, Masanori Hulse, Richard Phillip Bates, David Owen Donaldson, Lucy Frances |
| author_facet | Beazley-Long, Nicholas Moss, Catherine Elizabeth Ashby, William Robert Bestall, Samuel Marcus Almahasneh, Fatimah Durrant, Alexandra Margaret Benest, Andrew Vaughan Blackley, Zoe Ballmer-Hofer, Kurt Hirashima, Masanori Hulse, Richard Phillip Bates, David Owen Donaldson, Lucy Frances |
| author_sort | Beazley-Long, Nicholas |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Chronic pain can develop in response to conditions such as inflammatory arthritis. The central mechanisms underlying the development and maintenance of chronic pain in humans are not well elucidated although there is evidence for a role of microglia and astrocytes. However in pre-clinical models of pain, including models of inflammatory arthritis, there is a wealth of evidence indicating roles for pathological glial reactivity within the CNS. In the spinal dorsal horn of rats with painful inflammatory arthritis we found both a significant increase in CD11b+ microglia-like cells and GFAP+ astrocytes associated with blood vessels, and the number of activated blood vessels expressing the adhesion molecule ICAM-1, indicating potential glio-vascular activation. Using pharmacological interventions targeting VEGFR2 in arthritic rats, to inhibit endothelial cell activation, the number of dorsal horn ICAM-1+ blood vessels, CD11b+ microglia and the development of secondary mechanical allodynia, an indicator of central sensitization, were all prevented. Targeting endothelial VEGFR2 by inducible Tie2-specific VEGFR2 knock-out also prevented secondary allodynia in mice and glio-vascular activation in the dorsal horn in response to inflammatory arthritis. Inhibition of VEGFR2 in vitro significantly blocked ICAM-1-dependent monocyte adhesion to brain microvascular endothelial cells, when stimulated with inflammatory mediators TNF-α and VEGF-A165a. Taken together our findings suggest that a novel VEGFR2-mediated spinal cord glio-vascular mechanism may promote peripheral CD11b+ circulating cell transmigration into the CNS parenchyma and contribute to the development of chronic pain in inflammatory arthritis. We hypothesise that preventing this glio-vascular activation and circulating cell translocation into the spinal cord could be a new therapeutic strategy for pain caused by rheumatoid arthritis. |
| first_indexed | 2025-11-14T20:20:41Z |
| format | Article |
| id | nottingham-51439 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:20:41Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-514392019-01-24T08:36:05Z https://eprints.nottingham.ac.uk/51439/ VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis Beazley-Long, Nicholas Moss, Catherine Elizabeth Ashby, William Robert Bestall, Samuel Marcus Almahasneh, Fatimah Durrant, Alexandra Margaret Benest, Andrew Vaughan Blackley, Zoe Ballmer-Hofer, Kurt Hirashima, Masanori Hulse, Richard Phillip Bates, David Owen Donaldson, Lucy Frances Chronic pain can develop in response to conditions such as inflammatory arthritis. The central mechanisms underlying the development and maintenance of chronic pain in humans are not well elucidated although there is evidence for a role of microglia and astrocytes. However in pre-clinical models of pain, including models of inflammatory arthritis, there is a wealth of evidence indicating roles for pathological glial reactivity within the CNS. In the spinal dorsal horn of rats with painful inflammatory arthritis we found both a significant increase in CD11b+ microglia-like cells and GFAP+ astrocytes associated with blood vessels, and the number of activated blood vessels expressing the adhesion molecule ICAM-1, indicating potential glio-vascular activation. Using pharmacological interventions targeting VEGFR2 in arthritic rats, to inhibit endothelial cell activation, the number of dorsal horn ICAM-1+ blood vessels, CD11b+ microglia and the development of secondary mechanical allodynia, an indicator of central sensitization, were all prevented. Targeting endothelial VEGFR2 by inducible Tie2-specific VEGFR2 knock-out also prevented secondary allodynia in mice and glio-vascular activation in the dorsal horn in response to inflammatory arthritis. Inhibition of VEGFR2 in vitro significantly blocked ICAM-1-dependent monocyte adhesion to brain microvascular endothelial cells, when stimulated with inflammatory mediators TNF-α and VEGF-A165a. Taken together our findings suggest that a novel VEGFR2-mediated spinal cord glio-vascular mechanism may promote peripheral CD11b+ circulating cell transmigration into the CNS parenchyma and contribute to the development of chronic pain in inflammatory arthritis. We hypothesise that preventing this glio-vascular activation and circulating cell translocation into the spinal cord could be a new therapeutic strategy for pain caused by rheumatoid arthritis. Elsevier 2018-03-14 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/51439/8/1-s2.0-S0889159118300606-main.pdf Beazley-Long, Nicholas, Moss, Catherine Elizabeth, Ashby, William Robert, Bestall, Samuel Marcus, Almahasneh, Fatimah, Durrant, Alexandra Margaret, Benest, Andrew Vaughan, Blackley, Zoe, Ballmer-Hofer, Kurt, Hirashima, Masanori, Hulse, Richard Phillip, Bates, David Owen and Donaldson, Lucy Frances (2018) VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis. Brain, Behavior and Immunity, 74 . pp. 49-67. ISSN 1090-2139 Inflammatory pain; Rheumatoid arthritis; Chronic pain; Mechanical allodynia; VEGFR2; Glio-vascular activation; ICAM-1; CD11b; Microglia mono-arthritis https://www.sciencedirect.com/science/article/pii/S0889159118300606 doi:10.1016/j.bbi.2018.03.012 doi:10.1016/j.bbi.2018.03.012 |
| spellingShingle | Inflammatory pain; Rheumatoid arthritis; Chronic pain; Mechanical allodynia; VEGFR2; Glio-vascular activation; ICAM-1; CD11b; Microglia mono-arthritis Beazley-Long, Nicholas Moss, Catherine Elizabeth Ashby, William Robert Bestall, Samuel Marcus Almahasneh, Fatimah Durrant, Alexandra Margaret Benest, Andrew Vaughan Blackley, Zoe Ballmer-Hofer, Kurt Hirashima, Masanori Hulse, Richard Phillip Bates, David Owen Donaldson, Lucy Frances VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis |
| title | VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis |
| title_full | VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis |
| title_fullStr | VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis |
| title_full_unstemmed | VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis |
| title_short | VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis |
| title_sort | vegfr2 promotes central endothelial activation and the spread of pain in inflammatory arthritis |
| topic | Inflammatory pain; Rheumatoid arthritis; Chronic pain; Mechanical allodynia; VEGFR2; Glio-vascular activation; ICAM-1; CD11b; Microglia mono-arthritis |
| url | https://eprints.nottingham.ac.uk/51439/ https://eprints.nottingham.ac.uk/51439/ https://eprints.nottingham.ac.uk/51439/ |