Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses
Calcium (Ca) is an essential macronutrient for plants and its homeostasis is basic for many processes in plants. Therefore, both Ca deficiency and toxicity constitute potential issues for crops. CAX1 transporter is a potential target to obtain plants with better Ca homeostasis and higher Ca concentr...
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | https://eprints.nottingham.ac.uk/51372/ |
| _version_ | 1848798482034327552 |
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| author | Navarro-León, Eloy Ruiz, Juan Manuel Graham, Neil Blasco, Begoña |
| author_facet | Navarro-León, Eloy Ruiz, Juan Manuel Graham, Neil Blasco, Begoña |
| author_sort | Navarro-León, Eloy |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Calcium (Ca) is an essential macronutrient for plants and its homeostasis is basic for many processes in plants. Therefore, both Ca deficiency and toxicity constitute potential issues for crops. CAX1 transporter is a potential target to obtain plants with better Ca homeostasis and higher Ca concentration in edible parts. Three Brassica rapa mutants for CAX1 were obtained through TILLING. The objective of this work is to evaluate the growth, physiological state and nutrients concentration of these mutants grown with different Ca doses. The mutants and the parental line were grown under low, control and high Ca doses and parameters related to their oxidative stress, photosynthetic performance and nutrients concentration were determined. BraA.cax1a-4 and BraA.cax1a-7 mutants presented lower total Chl, an altered photosynthesis performance and higher ROS levels. BraA.cax1a-12 mutant grew better under high Ca conditions. All mutants accumulated more Ca and Mg in leaves under control and high Ca doses and accumulated more Fe regardless the Ca dose. The results obtained point to BraA.cax1a-12 as a potential candidate for biofortification with Fe, Ca and Mg since it accumulate higher concentrations of these elements, do not present an altered growth and is able to tolerate higher Ca doses. |
| first_indexed | 2025-11-14T20:20:28Z |
| format | Article |
| id | nottingham-51372 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:20:28Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
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| spelling | nottingham-513722019-04-24T04:30:12Z https://eprints.nottingham.ac.uk/51372/ Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses Navarro-León, Eloy Ruiz, Juan Manuel Graham, Neil Blasco, Begoña Calcium (Ca) is an essential macronutrient for plants and its homeostasis is basic for many processes in plants. Therefore, both Ca deficiency and toxicity constitute potential issues for crops. CAX1 transporter is a potential target to obtain plants with better Ca homeostasis and higher Ca concentration in edible parts. Three Brassica rapa mutants for CAX1 were obtained through TILLING. The objective of this work is to evaluate the growth, physiological state and nutrients concentration of these mutants grown with different Ca doses. The mutants and the parental line were grown under low, control and high Ca doses and parameters related to their oxidative stress, photosynthetic performance and nutrients concentration were determined. BraA.cax1a-4 and BraA.cax1a-7 mutants presented lower total Chl, an altered photosynthesis performance and higher ROS levels. BraA.cax1a-12 mutant grew better under high Ca conditions. All mutants accumulated more Ca and Mg in leaves under control and high Ca doses and accumulated more Fe regardless the Ca dose. The results obtained point to BraA.cax1a-12 as a potential candidate for biofortification with Fe, Ca and Mg since it accumulate higher concentrations of these elements, do not present an altered growth and is able to tolerate higher Ca doses. Elsevier 2018-04-24 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/51372/1/PlantScience_Leonetal2018.pdf Navarro-León, Eloy, Ruiz, Juan Manuel, Graham, Neil and Blasco, Begoña (2018) Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses. Plant Science . ISSN 0168-9452 https://www.sciencedirect.com/science/article/pii/S0168945218302681 doi:10.1016/j.plantsci.2018.04.019 doi:10.1016/j.plantsci.2018.04.019 |
| spellingShingle | Navarro-León, Eloy Ruiz, Juan Manuel Graham, Neil Blasco, Begoña Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses |
| title | Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses |
| title_full | Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses |
| title_fullStr | Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses |
| title_full_unstemmed | Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses |
| title_short | Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses |
| title_sort | physiological profile of cax1a tilling mutants of brassica rapa exposed to different calcium doses |
| url | https://eprints.nottingham.ac.uk/51372/ https://eprints.nottingham.ac.uk/51372/ https://eprints.nottingham.ac.uk/51372/ |