Anaerobic radical enzymes for biotechnology

Enzymes that proceed through radical intermediates have a rich chemistry that includes functionalisation of otherwise unreactive carbon atoms, carbon-skeleton rearrangements, aromatic reductions, and unusual eliminations. Especially under anaerobic conditions, organisms have developed a wide range o...

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Main Authors: Jäger, Christof M., Croft, Anna K.
Format: Article
Published: Wiley 2018
Subjects:
Online Access:https://eprints.nottingham.ac.uk/51326/
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author Jäger, Christof M.
Croft, Anna K.
author_facet Jäger, Christof M.
Croft, Anna K.
author_sort Jäger, Christof M.
building Nottingham Research Data Repository
collection Online Access
description Enzymes that proceed through radical intermediates have a rich chemistry that includes functionalisation of otherwise unreactive carbon atoms, carbon-skeleton rearrangements, aromatic reductions, and unusual eliminations. Especially under anaerobic conditions, organisms have developed a wide range of approaches for managing these transformations that can be exploited to generate new biological routes towards both bulk and specialty chemicals. These routes are often either much more direct or allow access to molecules that are inaccessible through standard (bio)chemical approaches. This review gives an overview of some of the key enzymes in this area: benzoyl-CoA reductases (that effect the enzymatic Birch reduction), ketyl radical dehydratases, coenzyme B12-dependant enzymes, glycyl radical enzymes, and radical SAM (AdoMet radical) enzymes. These enzymes are discussed alongside biotechnological applications, highlighting the wide range of actual and potential uses. With the increased diversity in biotechnological approaches to obtaining these enzymes and information about them, even more of these amazing enzymes can be expected to find application in industrial processes.
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spelling nottingham-513262020-05-04T19:33:40Z https://eprints.nottingham.ac.uk/51326/ Anaerobic radical enzymes for biotechnology Jäger, Christof M. Croft, Anna K. Enzymes that proceed through radical intermediates have a rich chemistry that includes functionalisation of otherwise unreactive carbon atoms, carbon-skeleton rearrangements, aromatic reductions, and unusual eliminations. Especially under anaerobic conditions, organisms have developed a wide range of approaches for managing these transformations that can be exploited to generate new biological routes towards both bulk and specialty chemicals. These routes are often either much more direct or allow access to molecules that are inaccessible through standard (bio)chemical approaches. This review gives an overview of some of the key enzymes in this area: benzoyl-CoA reductases (that effect the enzymatic Birch reduction), ketyl radical dehydratases, coenzyme B12-dependant enzymes, glycyl radical enzymes, and radical SAM (AdoMet radical) enzymes. These enzymes are discussed alongside biotechnological applications, highlighting the wide range of actual and potential uses. With the increased diversity in biotechnological approaches to obtaining these enzymes and information about them, even more of these amazing enzymes can be expected to find application in industrial processes. Wiley 2018-04-26 Article PeerReviewed Jäger, Christof M. and Croft, Anna K. (2018) Anaerobic radical enzymes for biotechnology. ChemBioEng Reviews, 5 (3). ISSN 2196-9744 Radicals Enzymes Catalysis Biotechnology Anaerobic reactions https://onlinelibrary.wiley.com/doi/abs/10.1002/cben.201800003 doi:10.1002/cben.201800003 doi:10.1002/cben.201800003
spellingShingle Radicals
Enzymes
Catalysis
Biotechnology
Anaerobic reactions
Jäger, Christof M.
Croft, Anna K.
Anaerobic radical enzymes for biotechnology
title Anaerobic radical enzymes for biotechnology
title_full Anaerobic radical enzymes for biotechnology
title_fullStr Anaerobic radical enzymes for biotechnology
title_full_unstemmed Anaerobic radical enzymes for biotechnology
title_short Anaerobic radical enzymes for biotechnology
title_sort anaerobic radical enzymes for biotechnology
topic Radicals
Enzymes
Catalysis
Biotechnology
Anaerobic reactions
url https://eprints.nottingham.ac.uk/51326/
https://eprints.nottingham.ac.uk/51326/
https://eprints.nottingham.ac.uk/51326/