Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals
The spatial organisation of Orai channels and SERCA pumps within ER-PM junctions is important for enhancing the versatility and specificity of subcellular Ca2+ signals generated during store operated Ca2+ entry (SOCE). In this paper we present a novel three dimensional spatio-temporal model describi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Published: |
Elsevier
2018
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/51146/ |
| _version_ | 1848798428074606592 |
|---|---|
| author | McIvor, Emma Coombes, Stephen Thul, Ruediger |
| author_facet | McIvor, Emma Coombes, Stephen Thul, Ruediger |
| author_sort | McIvor, Emma |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The spatial organisation of Orai channels and SERCA pumps within ER-PM junctions is important for enhancing the versatility and specificity of subcellular Ca2+ signals generated during store operated Ca2+ entry (SOCE). In this paper we present a novel three dimensional spatio-temporal model describing Ca2+ dynamics in the ER-PM junction and sub-PM ER during SOCE. We investigate the role of Orai channel and SERCA pump location to provide insights into how these components shape the Ca2+ signals generated and affect ER refilling. We find that the organisation of Orai channels within the ER-PM junction controls the amplitude and shape of the Ca2+ profile but does not enhance ER refilling. The model shows that ER refilling is only weakly affected by the location of SERCA2b pumps within the ER-PM junction and that the placement of SERCA2a pumps within the ER-PM junction has much greater control over ER refilling. |
| first_indexed | 2025-11-14T20:19:36Z |
| format | Article |
| id | nottingham-51146 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:19:36Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-511462020-05-04T19:47:44Z https://eprints.nottingham.ac.uk/51146/ Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals McIvor, Emma Coombes, Stephen Thul, Ruediger The spatial organisation of Orai channels and SERCA pumps within ER-PM junctions is important for enhancing the versatility and specificity of subcellular Ca2+ signals generated during store operated Ca2+ entry (SOCE). In this paper we present a novel three dimensional spatio-temporal model describing Ca2+ dynamics in the ER-PM junction and sub-PM ER during SOCE. We investigate the role of Orai channel and SERCA pump location to provide insights into how these components shape the Ca2+ signals generated and affect ER refilling. We find that the organisation of Orai channels within the ER-PM junction controls the amplitude and shape of the Ca2+ profile but does not enhance ER refilling. The model shows that ER refilling is only weakly affected by the location of SERCA2b pumps within the ER-PM junction and that the placement of SERCA2a pumps within the ER-PM junction has much greater control over ER refilling. Elsevier 2018-07-31 Article PeerReviewed McIvor, Emma, Coombes, Stephen and Thul, Ruediger (2018) Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals. Cell Calcium, 73 . pp. 11-24. ISSN 1532-1991 Calcium signalling; ER-PM junctions; Mathematical modelling; Spatial Ca2+ signature; Orai channels; ER refilling https://www.sciencedirect.com/science/article/pii/S0143416018300149 doi:10.1016/j.ceca.2018.03.006 doi:10.1016/j.ceca.2018.03.006 |
| spellingShingle | Calcium signalling; ER-PM junctions; Mathematical modelling; Spatial Ca2+ signature; Orai channels; ER refilling McIvor, Emma Coombes, Stephen Thul, Ruediger Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals |
| title | Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals |
| title_full | Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals |
| title_fullStr | Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals |
| title_full_unstemmed | Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals |
| title_short | Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals |
| title_sort | three-dimensional spatio-temporal modelling of store operated ca2+ entry: insights into er refilling and the spatial signature of ca2+ signals |
| topic | Calcium signalling; ER-PM junctions; Mathematical modelling; Spatial Ca2+ signature; Orai channels; ER refilling |
| url | https://eprints.nottingham.ac.uk/51146/ https://eprints.nottingham.ac.uk/51146/ https://eprints.nottingham.ac.uk/51146/ |