Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show...

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Main Authors: Giri, Jitender, Bhosale, Rahul, Huang, Guoqiang, Pandey, Bipin K., Parker, Helen, Zappala, Susan, Yang, Jing, Dievart, Anne, Bureau, Charlotte, Ljung, Karin, Price, Adam, Rose, Terry, Larrieu, Antoine, Mairhofer, Stefan, Sturrock, Craig J., White, Philip, Dupuy, Lionel, Hawkesford, Malcolm, Perin, Christophe, Liang, Wanqi, Peret, Benjamin, Hodgman, Charlie T., Lynch, Jonathan, Wissuwa, Matthias, Zhang, Dabing, Pridmore, Tony, Mooney, Sacha J., Guiderdoni, Emmanuel, Swarup, Ranjan, Bennett, Malcolm J.
Format: Article
Language:English
Published: Nature Publishing Group 2018
Online Access:https://eprints.nottingham.ac.uk/51120/
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author Giri, Jitender
Bhosale, Rahul
Huang, Guoqiang
Pandey, Bipin K.
Parker, Helen
Zappala, Susan
Yang, Jing
Dievart, Anne
Bureau, Charlotte
Ljung, Karin
Price, Adam
Rose, Terry
Larrieu, Antoine
Mairhofer, Stefan
Sturrock, Craig J.
White, Philip
Dupuy, Lionel
Hawkesford, Malcolm
Perin, Christophe
Liang, Wanqi
Peret, Benjamin
Hodgman, Charlie T.
Lynch, Jonathan
Wissuwa, Matthias
Zhang, Dabing
Pridmore, Tony
Mooney, Sacha J.
Guiderdoni, Emmanuel
Swarup, Ranjan
Bennett, Malcolm J.
author_facet Giri, Jitender
Bhosale, Rahul
Huang, Guoqiang
Pandey, Bipin K.
Parker, Helen
Zappala, Susan
Yang, Jing
Dievart, Anne
Bureau, Charlotte
Ljung, Karin
Price, Adam
Rose, Terry
Larrieu, Antoine
Mairhofer, Stefan
Sturrock, Craig J.
White, Philip
Dupuy, Lionel
Hawkesford, Malcolm
Perin, Christophe
Liang, Wanqi
Peret, Benjamin
Hodgman, Charlie T.
Lynch, Jonathan
Wissuwa, Matthias
Zhang, Dabing
Pridmore, Tony
Mooney, Sacha J.
Guiderdoni, Emmanuel
Swarup, Ranjan
Bennett, Malcolm J.
author_sort Giri, Jitender
building Nottingham Research Data Repository
collection Online Access
description Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.
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spelling nottingham-511202018-04-13T05:33:46Z https://eprints.nottingham.ac.uk/51120/ Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate Giri, Jitender Bhosale, Rahul Huang, Guoqiang Pandey, Bipin K. Parker, Helen Zappala, Susan Yang, Jing Dievart, Anne Bureau, Charlotte Ljung, Karin Price, Adam Rose, Terry Larrieu, Antoine Mairhofer, Stefan Sturrock, Craig J. White, Philip Dupuy, Lionel Hawkesford, Malcolm Perin, Christophe Liang, Wanqi Peret, Benjamin Hodgman, Charlie T. Lynch, Jonathan Wissuwa, Matthias Zhang, Dabing Pridmore, Tony Mooney, Sacha J. Guiderdoni, Emmanuel Swarup, Ranjan Bennett, Malcolm J. Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice. Nature Publishing Group 2018-04-12 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/51120/1/s41467-018-03850-4.pdf Giri, Jitender, Bhosale, Rahul, Huang, Guoqiang, Pandey, Bipin K., Parker, Helen, Zappala, Susan, Yang, Jing, Dievart, Anne, Bureau, Charlotte, Ljung, Karin, Price, Adam, Rose, Terry, Larrieu, Antoine, Mairhofer, Stefan, Sturrock, Craig J., White, Philip, Dupuy, Lionel, Hawkesford, Malcolm, Perin, Christophe, Liang, Wanqi, Peret, Benjamin, Hodgman, Charlie T., Lynch, Jonathan, Wissuwa, Matthias, Zhang, Dabing, Pridmore, Tony, Mooney, Sacha J., Guiderdoni, Emmanuel, Swarup, Ranjan and Bennett, Malcolm J. (2018) Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate. Nature Communications, 9 (1). 1408/1-1408/7. ISSN 2041-1723 https://www.nature.com/articles/s41467-018-03850-4 doi10.1038/s41467-018-03850-4 doi10.1038/s41467-018-03850-4
spellingShingle Giri, Jitender
Bhosale, Rahul
Huang, Guoqiang
Pandey, Bipin K.
Parker, Helen
Zappala, Susan
Yang, Jing
Dievart, Anne
Bureau, Charlotte
Ljung, Karin
Price, Adam
Rose, Terry
Larrieu, Antoine
Mairhofer, Stefan
Sturrock, Craig J.
White, Philip
Dupuy, Lionel
Hawkesford, Malcolm
Perin, Christophe
Liang, Wanqi
Peret, Benjamin
Hodgman, Charlie T.
Lynch, Jonathan
Wissuwa, Matthias
Zhang, Dabing
Pridmore, Tony
Mooney, Sacha J.
Guiderdoni, Emmanuel
Swarup, Ranjan
Bennett, Malcolm J.
Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate
title Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate
title_full Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate
title_fullStr Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate
title_full_unstemmed Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate
title_short Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate
title_sort rice auxin influx carrier osaux1 facilitates root hair elongation in response to low external phosphate
url https://eprints.nottingham.ac.uk/51120/
https://eprints.nottingham.ac.uk/51120/
https://eprints.nottingham.ac.uk/51120/