Tunable pentapeptide self-assembled β-sheet hydrogels
Oligopeptide-based supramolecular hydrogels hold promise in a range of applications. The gelation of these systems is hard to control with minor alterations in the peptide sequence significantly influencing the self-assembly process. This makes sequence design difficult whereby typical self-assembly...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
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Wiley-VCH Verlag
2018
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| Online Access: | https://eprints.nottingham.ac.uk/51067/ |
| _version_ | 1848798406529515520 |
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| author | Clarke, David E. Parmenter, Christopher D. J. Scherman, Oren A. |
| author_facet | Clarke, David E. Parmenter, Christopher D. J. Scherman, Oren A. |
| author_sort | Clarke, David E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Oligopeptide-based supramolecular hydrogels hold promise in a range of applications. The gelation of these systems is hard to control with minor alterations in the peptide sequence significantly influencing the self-assembly process. This makes sequence design difficult whereby typical self-assembly rules cannot be applied. We explored the design of pentapeptide sequences with different charge distributions and discovered that they formed robust, pH-responsive hydrogels. Through altering the concentration and charge distribution of the peptide sequence, we demonstrated that the stiffness of the hydrogels can be tuned across two orders of magnitude (2-200 kPa). Also, through the reassembly of the b-sheet interactions, the hydrogels can both selfheal and shear thin. Using spectroscopic and cryo-imaging techniques, we investigated the relationship between peptide sequence, molecular structure and how these influence the mechanical properties of the hydrogel. These pentapetide hydrogels attributed with tunable morphology and mechanical properties have promise in tissue engineering, injectable delivery vectors and 3D printing applications. |
| first_indexed | 2025-11-14T20:19:16Z |
| format | Article |
| id | nottingham-51067 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T20:19:16Z |
| publishDate | 2018 |
| publisher | Wiley-VCH Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-510672019-03-30T04:30:14Z https://eprints.nottingham.ac.uk/51067/ Tunable pentapeptide self-assembled β-sheet hydrogels Clarke, David E. Parmenter, Christopher D. J. Scherman, Oren A. Oligopeptide-based supramolecular hydrogels hold promise in a range of applications. The gelation of these systems is hard to control with minor alterations in the peptide sequence significantly influencing the self-assembly process. This makes sequence design difficult whereby typical self-assembly rules cannot be applied. We explored the design of pentapeptide sequences with different charge distributions and discovered that they formed robust, pH-responsive hydrogels. Through altering the concentration and charge distribution of the peptide sequence, we demonstrated that the stiffness of the hydrogels can be tuned across two orders of magnitude (2-200 kPa). Also, through the reassembly of the b-sheet interactions, the hydrogels can both selfheal and shear thin. Using spectroscopic and cryo-imaging techniques, we investigated the relationship between peptide sequence, molecular structure and how these influence the mechanical properties of the hydrogel. These pentapetide hydrogels attributed with tunable morphology and mechanical properties have promise in tissue engineering, injectable delivery vectors and 3D printing applications. Wiley-VCH Verlag 2018-03-30 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/51067/1/Accepted%20Article_Angewante_Clarke%20et%20al.pdf application/pdf en https://eprints.nottingham.ac.uk/51067/2/Supporting%20Information%20for%20Accepted%20Article_Angewante_Clarke%20et%20al_.pdf Clarke, David E., Parmenter, Christopher D. J. and Scherman, Oren A. (2018) Tunable pentapeptide self-assembled β-sheet hydrogels. Angewandte Chemie International Edition . ISSN 1521-3773 https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201801001 doi:10.1002/anie.201801001 doi:10.1002/anie.201801001 |
| spellingShingle | Clarke, David E. Parmenter, Christopher D. J. Scherman, Oren A. Tunable pentapeptide self-assembled β-sheet hydrogels |
| title | Tunable pentapeptide self-assembled β-sheet hydrogels |
| title_full | Tunable pentapeptide self-assembled β-sheet hydrogels |
| title_fullStr | Tunable pentapeptide self-assembled β-sheet hydrogels |
| title_full_unstemmed | Tunable pentapeptide self-assembled β-sheet hydrogels |
| title_short | Tunable pentapeptide self-assembled β-sheet hydrogels |
| title_sort | tunable pentapeptide self-assembled β-sheet hydrogels |
| url | https://eprints.nottingham.ac.uk/51067/ https://eprints.nottingham.ac.uk/51067/ https://eprints.nottingham.ac.uk/51067/ |