The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis
BACKGROUND: As only a minor portion of the information present in histological sections is accessible by eye, recognition and quantification of complex patterns and relationships among constituents relies on digital image analysis. In this study, our working hypothesis was that, with the application...
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BioMed Central
2018
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| Online Access: | https://eprints.nottingham.ac.uk/50701/ |
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| author | Roxanis, I. Colling, R. Kartsonaki, C. Green, A.R. Rakha, E.A. |
| author_facet | Roxanis, I. Colling, R. Kartsonaki, C. Green, A.R. Rakha, E.A. |
| author_sort | Roxanis, I. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | BACKGROUND: As only a minor portion of the information present in histological sections is accessible by eye, recognition and quantification of complex patterns and relationships among constituents relies on digital image analysis. In this study, our working hypothesis was that, with the application of digital image analysis technology, visually unquantifiable breast cancer microarchitectural features can be rigorously assessed and tested as prognostic parameters for invasive breast carcinoma of no special type.
METHODS: Digital image analysis was performed using public domain software (ImageJ) on tissue microarrays from a cohort of 696 patients, and validated with a commercial platform (Visiopharm). Quantified features included elements defining tumour microarchitecture, with emphasis on the extent of tumour-stroma interface. The differential prognostic impact of tumour nest microarchitecture in the four immunohistochemical surrogates for molecular classification was analysed. Prognostic parameters included axillary lymph node status, breast cancer-specific survival, and time to distant metastasis. Associations of each feature with prognostic parameters were assessed using logistic regression and Cox proportional models adjusting for age at diagnosis, grade, and tumour size.
RESULTS: An arrangement in numerous small nests was associated with axillary lymph node involvement. The association was stronger in luminal tumours (odds ratio (OR) = 1.39, p = 0.003 for a 1-SD increase in nest number, OR = 0.75, p = 0.006 for mean nest area). Nest number was also associated with survival (hazard ratio (HR) = 1.15, p = 0.027), but total nest perimeter was the parameter most significantly associated with survival in luminal tumours (HR = 1.26, p = 0.005). In the relatively small cohort of triple-negative tumours, mean circularity showed association with time to distant metastasis (HR = 1.71, p = 0.027) and survival (HR = 1.8, p = 0.02).
CONCLUSIONS: We propose that tumour arrangement in few large nests indicates a decreased metastatic potential. By contrast, organisation in numerous small nests provides the tumour with increased metastatic potential to regional lymph nodes. An outstretched pattern in small nests bestows tumours with a tendency for decreased breast cancer-specific survival. Although further validation studies are required before the argument for routine quantification of microarchitectural features is established, our approach is consistent with the demand for cost-effective methods for triaging breast cancer patients that are more likely to benefit from chemotherapy. |
| first_indexed | 2025-11-14T20:17:52Z |
| format | Article |
| id | nottingham-50701 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:17:52Z |
| publishDate | 2018 |
| publisher | BioMed Central |
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| spelling | nottingham-507012020-05-04T19:31:05Z https://eprints.nottingham.ac.uk/50701/ The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis Roxanis, I. Colling, R. Kartsonaki, C. Green, A.R. Rakha, E.A. BACKGROUND: As only a minor portion of the information present in histological sections is accessible by eye, recognition and quantification of complex patterns and relationships among constituents relies on digital image analysis. In this study, our working hypothesis was that, with the application of digital image analysis technology, visually unquantifiable breast cancer microarchitectural features can be rigorously assessed and tested as prognostic parameters for invasive breast carcinoma of no special type. METHODS: Digital image analysis was performed using public domain software (ImageJ) on tissue microarrays from a cohort of 696 patients, and validated with a commercial platform (Visiopharm). Quantified features included elements defining tumour microarchitecture, with emphasis on the extent of tumour-stroma interface. The differential prognostic impact of tumour nest microarchitecture in the four immunohistochemical surrogates for molecular classification was analysed. Prognostic parameters included axillary lymph node status, breast cancer-specific survival, and time to distant metastasis. Associations of each feature with prognostic parameters were assessed using logistic regression and Cox proportional models adjusting for age at diagnosis, grade, and tumour size. RESULTS: An arrangement in numerous small nests was associated with axillary lymph node involvement. The association was stronger in luminal tumours (odds ratio (OR) = 1.39, p = 0.003 for a 1-SD increase in nest number, OR = 0.75, p = 0.006 for mean nest area). Nest number was also associated with survival (hazard ratio (HR) = 1.15, p = 0.027), but total nest perimeter was the parameter most significantly associated with survival in luminal tumours (HR = 1.26, p = 0.005). In the relatively small cohort of triple-negative tumours, mean circularity showed association with time to distant metastasis (HR = 1.71, p = 0.027) and survival (HR = 1.8, p = 0.02). CONCLUSIONS: We propose that tumour arrangement in few large nests indicates a decreased metastatic potential. By contrast, organisation in numerous small nests provides the tumour with increased metastatic potential to regional lymph nodes. An outstretched pattern in small nests bestows tumours with a tendency for decreased breast cancer-specific survival. Although further validation studies are required before the argument for routine quantification of microarchitectural features is established, our approach is consistent with the demand for cost-effective methods for triaging breast cancer patients that are more likely to benefit from chemotherapy. BioMed Central 2018-02-05 Article PeerReviewed Roxanis, I., Colling, R., Kartsonaki, C., Green, A.R. and Rakha, E.A. (2018) The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis. Breast Cancer Research, 20 (11). pp. 1-11. ISSN 1465-542X Breast cancer; Digital image analysis; Prognosis; Microarchitecture; Tumour nests https://breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-0934-x doi:10.1186/s13058-018-0934-x doi:10.1186/s13058-018-0934-x |
| spellingShingle | Breast cancer; Digital image analysis; Prognosis; Microarchitecture; Tumour nests Roxanis, I. Colling, R. Kartsonaki, C. Green, A.R. Rakha, E.A. The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis |
| title | The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis |
| title_full | The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis |
| title_fullStr | The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis |
| title_full_unstemmed | The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis |
| title_short | The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis |
| title_sort | significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis |
| topic | Breast cancer; Digital image analysis; Prognosis; Microarchitecture; Tumour nests |
| url | https://eprints.nottingham.ac.uk/50701/ https://eprints.nottingham.ac.uk/50701/ https://eprints.nottingham.ac.uk/50701/ |