The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus
Dichelobacter nodosus (D. nodosus) is the causative pathogen of ovine footrot, a disease that has a significant welfare and financial impact on the global sheep industry. Previous studies into the phylogenetics of D. nodosus have focused on Australia and Scandinavia, meaning the current diversity in...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers
2018
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| Online Access: | https://eprints.nottingham.ac.uk/50510/ |
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| author | Blanchard, Adam M. Jolley, Keith A. Maiden, Martin C.J. Coffey, Tracey J. Maboni, Grazieli Staley, Ceri E. Bollard, N.J. Warry, Andrew Emes, Richard D. Davies, Peers Tötemeyer, Sabine |
| author_facet | Blanchard, Adam M. Jolley, Keith A. Maiden, Martin C.J. Coffey, Tracey J. Maboni, Grazieli Staley, Ceri E. Bollard, N.J. Warry, Andrew Emes, Richard D. Davies, Peers Tötemeyer, Sabine |
| author_sort | Blanchard, Adam M. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Dichelobacter nodosus (D. nodosus) is the causative pathogen of ovine footrot, a disease that has a significant welfare and financial impact on the global sheep industry. Previous studies into the phylogenetics of D. nodosus have focused on Australia and Scandinavia, meaning the current diversity in the United Kingdom (U.K.) population and its relationship globally, is poorly understood. Numerous epidemiological methods are available for bacterial typing; however, few account for whole genome diversity or provide the opportunity for future application of new computational techniques. Multilocus sequence typing (MLST) measures nucleotide variations within several loci with slow accumulation of variation to enable the designation of allele numbers to determine a sequence type. The usage of whole genome sequence data enables the application of MLST, but also core and whole genome MLST for higher levels of strain discrimination with a negligible increase in experimental cost. An MLST database was developed alongside a seven loci scheme using publically available whole genome data from the sequence read archive. Sequence type designation and strain discrimination was compared to previously published data to ensure reproducibility. Multiple D. nodosus isolates from U.K. farms were directly compared to populations from other countries. The U.K. isolates define new clades within the global population of D. nodosus and predominantly consist of serogroups A, B and H, however serogroups C, D, E, and I were also found. The
scheme is publically available at https://pubmlst.org/dnodosus/. |
| first_indexed | 2025-11-14T20:17:06Z |
| format | Article |
| id | nottingham-50510 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:17:06Z |
| publishDate | 2018 |
| publisher | Frontiers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-505102018-04-10T15:43:38Z https://eprints.nottingham.ac.uk/50510/ The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus Blanchard, Adam M. Jolley, Keith A. Maiden, Martin C.J. Coffey, Tracey J. Maboni, Grazieli Staley, Ceri E. Bollard, N.J. Warry, Andrew Emes, Richard D. Davies, Peers Tötemeyer, Sabine Dichelobacter nodosus (D. nodosus) is the causative pathogen of ovine footrot, a disease that has a significant welfare and financial impact on the global sheep industry. Previous studies into the phylogenetics of D. nodosus have focused on Australia and Scandinavia, meaning the current diversity in the United Kingdom (U.K.) population and its relationship globally, is poorly understood. Numerous epidemiological methods are available for bacterial typing; however, few account for whole genome diversity or provide the opportunity for future application of new computational techniques. Multilocus sequence typing (MLST) measures nucleotide variations within several loci with slow accumulation of variation to enable the designation of allele numbers to determine a sequence type. The usage of whole genome sequence data enables the application of MLST, but also core and whole genome MLST for higher levels of strain discrimination with a negligible increase in experimental cost. An MLST database was developed alongside a seven loci scheme using publically available whole genome data from the sequence read archive. Sequence type designation and strain discrimination was compared to previously published data to ensure reproducibility. Multiple D. nodosus isolates from U.K. farms were directly compared to populations from other countries. The U.K. isolates define new clades within the global population of D. nodosus and predominantly consist of serogroups A, B and H, however serogroups C, D, E, and I were also found. The scheme is publically available at https://pubmlst.org/dnodosus/. Frontiers 2018-03-23 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/50510/8/fmicb-09-00551.pdf Blanchard, Adam M., Jolley, Keith A., Maiden, Martin C.J., Coffey, Tracey J., Maboni, Grazieli, Staley, Ceri E., Bollard, N.J., Warry, Andrew, Emes, Richard D., Davies, Peers and Tötemeyer, Sabine (2018) The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus. Frontiers in Microbiology, 9 . 551/1-551/10. ISSN 1664-302X footrot Dichelobacter nodosus MLST genotyping core genome multilocus sequence typing whole genome multilocus sequence typing (wgMLST) cgMLST https://www.frontiersin.org/articles/10.3389/fmicb.2018.00551/full doi:10.3389/fmicb.2018.00551 doi:10.3389/fmicb.2018.00551 |
| spellingShingle | footrot Dichelobacter nodosus MLST genotyping core genome multilocus sequence typing whole genome multilocus sequence typing (wgMLST) cgMLST Blanchard, Adam M. Jolley, Keith A. Maiden, Martin C.J. Coffey, Tracey J. Maboni, Grazieli Staley, Ceri E. Bollard, N.J. Warry, Andrew Emes, Richard D. Davies, Peers Tötemeyer, Sabine The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus |
| title | The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus |
| title_full | The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus |
| title_fullStr | The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus |
| title_full_unstemmed | The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus |
| title_short | The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus |
| title_sort | applied development of a tiered multilocus sequence typing (mlst) scheme for dichelobacter nodosus |
| topic | footrot Dichelobacter nodosus MLST genotyping core genome multilocus sequence typing whole genome multilocus sequence typing (wgMLST) cgMLST |
| url | https://eprints.nottingham.ac.uk/50510/ https://eprints.nottingham.ac.uk/50510/ https://eprints.nottingham.ac.uk/50510/ |