Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation
The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1), for potential use as a consolidant for decayed archaeological wood, has been examined by sedimentation velocity and sedimentation equilibriumin the analytical ultracentrifug...
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| Format: | Article |
| Language: | English |
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Springer
2018
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| Online Access: | https://eprints.nottingham.ac.uk/50463/ |
| _version_ | 1848798257397891072 |
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| author | Wakefield, Jennifer M.K. Gillis, Richard B. Adams, Gary G. McQueen, Caitlin Harding, Stephen E. |
| author_facet | Wakefield, Jennifer M.K. Gillis, Richard B. Adams, Gary G. McQueen, Caitlin Harding, Stephen E. |
| author_sort | Wakefield, Jennifer M.K. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1), for potential use as a consolidant for decayed archaeological wood, has been examined by sedimentation velocity and sedimentation equilibriumin the analytical ultracentrifuge before and after depolymerisation. Sedimentation velocity before polymerisation revealed a uniform distribution of sedimentation coefficient with little concentration dependence. SEDFIT-MSTAR analysis revealed a weight average molecular weight Mw of (14.2 + 1.2) kDa, and polydispersity index of ~ 1.2. Further analysis using MULTISIG revealed a distribution of material between 2-20 kDa and consistent with the weight average Mw. Controlled depolymerisation using hydrogen peroxide and UV in an acetic acid medium reduced this to (4.9 + 0.7) kDa, with a similar polydispersity. The depolymerised material appears to be within the range that has been predicted to fully penetrate into archaeological wood. The consequences for this and the use of the analytical ultracentrifuge in wood conservation strategies is considered. |
| first_indexed | 2025-11-14T20:16:54Z |
| format | Article |
| id | nottingham-50463 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:16:54Z |
| publishDate | 2018 |
| publisher | Springer |
| recordtype | eprints |
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| spelling | nottingham-504632018-09-28T07:26:29Z https://eprints.nottingham.ac.uk/50463/ Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation Wakefield, Jennifer M.K. Gillis, Richard B. Adams, Gary G. McQueen, Caitlin Harding, Stephen E. The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1), for potential use as a consolidant for decayed archaeological wood, has been examined by sedimentation velocity and sedimentation equilibriumin the analytical ultracentrifuge before and after depolymerisation. Sedimentation velocity before polymerisation revealed a uniform distribution of sedimentation coefficient with little concentration dependence. SEDFIT-MSTAR analysis revealed a weight average molecular weight Mw of (14.2 + 1.2) kDa, and polydispersity index of ~ 1.2. Further analysis using MULTISIG revealed a distribution of material between 2-20 kDa and consistent with the weight average Mw. Controlled depolymerisation using hydrogen peroxide and UV in an acetic acid medium reduced this to (4.9 + 0.7) kDa, with a similar polydispersity. The depolymerised material appears to be within the range that has been predicted to fully penetrate into archaeological wood. The consequences for this and the use of the analytical ultracentrifuge in wood conservation strategies is considered. Springer 2018-10 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/50463/8/s00249-018-1290-6.pdf Wakefield, Jennifer M.K., Gillis, Richard B., Adams, Gary G., McQueen, Caitlin and Harding, Stephen E. (2018) Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation. European Biophysics Journal, 47 (7). pp. 769-775. ISSN 1432-1017 Analytical ultracentrifuge SEDFIT-MSTAR hydrogen peroxide-ultraviolet depolymerisation Oseberg artefacts https://link.springer.com/article/10.1007%2Fs00249-018-1290-6 doi:10.1007/s00249-018-1290-6 doi:10.1007/s00249-018-1290-6 |
| spellingShingle | Analytical ultracentrifuge SEDFIT-MSTAR hydrogen peroxide-ultraviolet depolymerisation Oseberg artefacts Wakefield, Jennifer M.K. Gillis, Richard B. Adams, Gary G. McQueen, Caitlin Harding, Stephen E. Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation |
| title | Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation |
| title_full | Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation |
| title_fullStr | Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation |
| title_full_unstemmed | Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation |
| title_short | Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation |
| title_sort | controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation |
| topic | Analytical ultracentrifuge SEDFIT-MSTAR hydrogen peroxide-ultraviolet depolymerisation Oseberg artefacts |
| url | https://eprints.nottingham.ac.uk/50463/ https://eprints.nottingham.ac.uk/50463/ https://eprints.nottingham.ac.uk/50463/ |