Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences

5-methylcytosine (5mC) is the best understood DNA modification and is generally believed to be associated with repression of gene expression. Over the last decade, sequentially oxidized forms of 5mC (oxi-mCs) have been discovered within the genomes of vertebrates. Their discovery was accompanied by...

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Main Authors: Jessop, Peter, Ruzov, Alexey, Gering, Martin
Format: Article
Language:English
Published: Frontiers Media 2018
Online Access:https://eprints.nottingham.ac.uk/50405/
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author Jessop, Peter
Ruzov, Alexey
Gering, Martin
author_facet Jessop, Peter
Ruzov, Alexey
Gering, Martin
author_sort Jessop, Peter
building Nottingham Research Data Repository
collection Online Access
description 5-methylcytosine (5mC) is the best understood DNA modification and is generally believed to be associated with repression of gene expression. Over the last decade, sequentially oxidized forms of 5mC (oxi-mCs) have been discovered within the genomes of vertebrates. Their discovery was accompanied by that of the ten-eleven translocation (TET) methylcytosine dioxygenases, the enzymes that catalyse the formation of the oxi-mCs. Although a number of studies performed on different vertebrate models and embryonic stem cells demonstrated that both TET enzymes and oxi-mCs are likely to be important for several developmental processes it is currently unclear whether their developmental roles are conserved among vertebrates. Here, we summarise recent developments in this field suggesting that biological roles of TETs/oxi-mCs may significantly differ between mice and zebrafish. Thus, although the role of TET proteins in late organogenesis has been documented for both these systems; unlike in mice the enzymatic oxidation of 5mC does not seem to be involved in zygotic reprogramming or gastrulation in zebrafish. Our analysis may provide an insight into the general principles of epigenetic regulation of animal development and cellular differentiation.
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spelling nottingham-504052020-05-08T09:30:09Z https://eprints.nottingham.ac.uk/50405/ Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences Jessop, Peter Ruzov, Alexey Gering, Martin 5-methylcytosine (5mC) is the best understood DNA modification and is generally believed to be associated with repression of gene expression. Over the last decade, sequentially oxidized forms of 5mC (oxi-mCs) have been discovered within the genomes of vertebrates. Their discovery was accompanied by that of the ten-eleven translocation (TET) methylcytosine dioxygenases, the enzymes that catalyse the formation of the oxi-mCs. Although a number of studies performed on different vertebrate models and embryonic stem cells demonstrated that both TET enzymes and oxi-mCs are likely to be important for several developmental processes it is currently unclear whether their developmental roles are conserved among vertebrates. Here, we summarise recent developments in this field suggesting that biological roles of TETs/oxi-mCs may significantly differ between mice and zebrafish. Thus, although the role of TET proteins in late organogenesis has been documented for both these systems; unlike in mice the enzymatic oxidation of 5mC does not seem to be involved in zygotic reprogramming or gastrulation in zebrafish. Our analysis may provide an insight into the general principles of epigenetic regulation of animal development and cellular differentiation. Frontiers Media 2018-03-04 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/50405/22/fcell-06-00027.pdf Jessop, Peter, Ruzov, Alexey and Gering, Martin (2018) Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences. Frontiers in Cell and Developmental Biology, 6 . 27/1-27/15. ISSN 2296-634X https://www.frontiersin.org/articles/10.3389/fcell.2018.00027/full doi:10.3389/fcell.2018.00027 doi:10.3389/fcell.2018.00027
spellingShingle Jessop, Peter
Ruzov, Alexey
Gering, Martin
Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences
title Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences
title_full Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences
title_fullStr Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences
title_full_unstemmed Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences
title_short Developmental functions of the dynamic DNA methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences
title_sort developmental functions of the dynamic dna methylome and hydroxymethylome in the mouse and zebrafish: similarities and differences
url https://eprints.nottingham.ac.uk/50405/
https://eprints.nottingham.ac.uk/50405/
https://eprints.nottingham.ac.uk/50405/