Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease
Background & Aims Chronic liver disease presents a major global public health challenge. Stratification of asymptomatic, at-risk patients in primary care using non-invasive methods has the potential to address this by identifying those likely to progress. We therefore evaluated variant alleles...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018
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| Online Access: | https://eprints.nottingham.ac.uk/50349/ |
| _version_ | 1848798228645937152 |
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| author | Grove, Jane I. Thiagarajan, Prarthana Astbury, Stuart Harris, Rebecca Delahooke, Toby Guha, Indra Neil Aithal, Guruprasad P. |
| author_facet | Grove, Jane I. Thiagarajan, Prarthana Astbury, Stuart Harris, Rebecca Delahooke, Toby Guha, Indra Neil Aithal, Guruprasad P. |
| author_sort | Grove, Jane I. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background & Aims
Chronic liver disease presents a major global public health challenge. Stratification of asymptomatic, at-risk patients in primary care using non-invasive methods has the potential to address this by identifying those likely to progress. We therefore evaluated variant alleles at loci associated with non-alcoholic fatty liver disease (NAFLD) as genetic determinants of substantial liver injury in patients with disease risk factors.
Methods
Levels of serum procollagen III (PIIINP), an established fibrosis and steatohepatitis marker, were determined in 467 people who had type 2 diabetes and/or BMI>27.3 (identified from registration with general practitioners) in this observational cross-sectional study. Patients were genotyped for characterised risk alleles in PNPLA3 (rs738409), GCKR (rs1260326) and TM6SF2 (rs58542926) and associations with PIIINP assessed.
Results
The risk alleles in PNPLA3, GCKR or TM6SF2 were not found to be individually associated with the presence of a disease risk factor and were not significantly more common in patients with raised serum PIIINP. The prevalence of possession of both PNPLA3 and GCKR variant alleles combined was significantly higher in at-risk patients with clinically significant liver disease indicated by serum PIIINP above 11 ng/ml (P=0.014).
Conclusions
Genotyping therefore has limited value for predicting severe liver disease in at-risk individuals identified in a community setting. |
| first_indexed | 2025-11-14T20:16:26Z |
| format | Article |
| id | nottingham-50349 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:16:26Z |
| publishDate | 2018 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-503492019-03-15T04:30:19Z https://eprints.nottingham.ac.uk/50349/ Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease Grove, Jane I. Thiagarajan, Prarthana Astbury, Stuart Harris, Rebecca Delahooke, Toby Guha, Indra Neil Aithal, Guruprasad P. Background & Aims Chronic liver disease presents a major global public health challenge. Stratification of asymptomatic, at-risk patients in primary care using non-invasive methods has the potential to address this by identifying those likely to progress. We therefore evaluated variant alleles at loci associated with non-alcoholic fatty liver disease (NAFLD) as genetic determinants of substantial liver injury in patients with disease risk factors. Methods Levels of serum procollagen III (PIIINP), an established fibrosis and steatohepatitis marker, were determined in 467 people who had type 2 diabetes and/or BMI>27.3 (identified from registration with general practitioners) in this observational cross-sectional study. Patients were genotyped for characterised risk alleles in PNPLA3 (rs738409), GCKR (rs1260326) and TM6SF2 (rs58542926) and associations with PIIINP assessed. Results The risk alleles in PNPLA3, GCKR or TM6SF2 were not found to be individually associated with the presence of a disease risk factor and were not significantly more common in patients with raised serum PIIINP. The prevalence of possession of both PNPLA3 and GCKR variant alleles combined was significantly higher in at-risk patients with clinically significant liver disease indicated by serum PIIINP above 11 ng/ml (P=0.014). Conclusions Genotyping therefore has limited value for predicting severe liver disease in at-risk individuals identified in a community setting. Wiley 2018-03-01 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/50349/1/FINAL%20upload%20liv%20int%20geno%20manuscript%20220218.pdf Grove, Jane I., Thiagarajan, Prarthana, Astbury, Stuart, Harris, Rebecca, Delahooke, Toby, Guha, Indra Neil and Aithal, Guruprasad P. (2018) Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease. Liver International . ISSN 1478-3231 PNPLA3 GCKR TM6SF2 NAFLD http://onlinelibrary.wiley.com/doi/10.1111/liv.13733/abstract doi:10.1111/liv.13733 doi:10.1111/liv.13733 |
| spellingShingle | PNPLA3 GCKR TM6SF2 NAFLD Grove, Jane I. Thiagarajan, Prarthana Astbury, Stuart Harris, Rebecca Delahooke, Toby Guha, Indra Neil Aithal, Guruprasad P. Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease |
| title | Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease |
| title_full | Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease |
| title_fullStr | Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease |
| title_full_unstemmed | Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease |
| title_short | Analysis of genotyping for predicting liver injury marker, Procollagen III in persons at risk of non-alcoholic fatty liver disease |
| title_sort | analysis of genotyping for predicting liver injury marker, procollagen iii in persons at risk of non-alcoholic fatty liver disease |
| topic | PNPLA3 GCKR TM6SF2 NAFLD |
| url | https://eprints.nottingham.ac.uk/50349/ https://eprints.nottingham.ac.uk/50349/ https://eprints.nottingham.ac.uk/50349/ |